LOCUS       sequence-399046-       13264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0449
FEATURES             Location/Qualifiers
     source          1..13264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9121..9709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9174..9194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9592..9603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9781..10014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10092..10213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10129..10148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10183..10202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10253..10388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10408..10427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10409..10427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10437..10453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10446..10468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10595..11050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10682..10701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10892..10913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11076..11180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11181..11249,11250..12041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11399..11418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12212..12800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12701..12720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12954..12971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13088..13109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13124..13129,13130..13147,13148..13154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13162..13178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13167..13189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13186..13202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13186..13202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13221..13241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13223..13241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccg gcgcgtaccg ccccagcccg
     4141 ggtgcccaca ctggccgtcc cggccatctc gctgcccgac gatgtccgca ggcggctcaa
     4201 agatttggaa agagacagct taacagaaaa ggaatgtgtg aaggagaaat tgaatctctt
     4261 gcacgaattt ctgcaaacag aaataaagaa tcagttatgt gacttggaaa ccaaattacg
     4321 taaagaagaa ttatccgagg agggctacct ggctaaagtc aaatcccttt taaataaaga
     4381 tttgtccttg gagaacggtg ctcatgctta caaccgggaa gtgaatggac gtctagaaaa
     4441 cgggaaccaa gcaagaagtg aagcccgtag agtgggaatg gcagatgcca acagcccccc
     4501 caaacccctt tccaaacctc gcacgcccag gaggagcaag tccgatggag aggctaagcg
     4561 ttcaagagac cctcctgcct cagcctccca agtaactggg attagagctg aaccttcacc
     4621 tagccccagg attacaagga aaagcaccag gcaaaccacc atcacatctc attttgcaaa
     4681 gggccctgcc aaacggaaac ctcaggaaga gtctgaaaga gccaaatcgg atgagtccat
     4741 caaggaagaa gacaaagacc aggatgagaa gagacgtaga gttacatcca gagaacgagt
     4801 tgctagaccg cttcctgcag aagaacctga aagagcaaaa tcaggaacgc gcactgaaaa
     4861 ggaagaagaa agagatgaaa aagaagaaaa gagactccga agtcaaacca aagaaccaac
     4921 acccaaacag aaactgaagg aggagccgga cagagaagcc agggcaggcg tgcaggctga
     4981 cgaggacgaa gatggagacg agaaagatga gaagaagcac agaagtcaac ccaaagatct
     5041 agctgccaaa cggaggcccg aagaaaaaga acctgaaaaa gtaaatccac agatttctga
     5101 tgaaaaagac gaggatgaaa aggaggagaa gagacgcaaa acgaccccca aagaaccaac
     5161 ggagaaaaaa atggctcgcg ccaaaacagt catgaactcc aagacccacc ctcccaagtg
     5221 cattcagtgc gggcagtacc tggacgaccc tgacctcaaa tatgggcagc acccaccaga
     5281 cgcggtggat gagccacaga tgctgacaaa tgagaagctg tccatctttg atgccaacga
     5341 gtctggcttt gagagttatg aggcgcttcc ccagcacaaa ctgacctgct tcagtgtgta
     5401 ctgtaagcac ggtcacctgt gtcccatcga caccggcctc atcgagaaga atatcgaact
     5461 cttcttttct ggttcagcaa aaccaatcta tgatgatgac ccatctcttg aaggtggtgt
     5521 taatggcaaa aatcttggcc ccataaatga atggtggatc actggctttg atggaggtga
     5581 aaaggccctc atcggcttca gcacctcatt tgccgaatac attctgatgg atcccagtcc
     5641 cgagtatgcg cccatatttg ggctgatgca ggagaagatc tacatcagca agattgtggt
     5701 ggagttcctg cagagcaatt ccgactcgac ctatgaggac ctgatcaaca agatcgagac
     5761 cacggttcct ccttctggcc tcaacttgaa ccgcttcaca gaggactccc tcctgcgaca
     5821 cgcgcagttt gtggtggagc aggtggagag ttatgacgag gccggggaca gtgatgagca
     5881 gcccatcttc ctgacaccct gcatgcggga cctgatcaag ctggctgggg tcacgctggg
     5941 acagaggcga gcccaggcga ggcggcagac catcaggcat tctaccaggg agaaggacag
     6001 gggacccacg aaagccacca ccaccaagct ggtctaccag atcttcgata ctttcttcgc
     6061 agagcaaatt gaaaaggatg acagagaaga caaggagaac gcctttaagc gccggcgatg
     6121 tggcgtctgt gaggtgtgtc agcagcctga gtgtgggaaa tgtaaagcct gcaaggacat
     6181 ggttaaattt ggtggcagtg gacggagcaa gcaggcttgc caagagcgga ggtgtcccaa
     6241 tatggccatg aaggaggcag atgacgatga ggaagtcgat gataacatcc cagagatgcc
     6301 gtcacccaaa aaaatgcacc aggggaagaa gaagaaacag aacaagaatc gcatctcttg
     6361 ggtcggagaa gccgtcaaga ctgatgggaa gaagagttac tataagaagg tgtgcattga
     6421 tgcggaaacc ctggaagtgg gggactgtgt ctctgttatt ccagatgatt cctcaaaacc
     6481 gctgtatcta gcaagggtca cggcgctgtg ggaggacagc agcaacgggc agatgtttca
     6541 cgcccactgg ttctgcgctg ggacagacac agtcctcggg gccacgtcgg accctctgga
     6601 gctgttcttg gtggatgaat gtgaggacat gcagctttca tatatccaca gcaaagtgaa
     6661 agtcatctac aaagccccct ccgaaaactg ggccatggag ggaggcatgg atcccgagtc
     6721 cctgctggag ggggacgacg ggaagaccta cttctaccag ctgtggtatg atcaagacta
     6781 cgcgagattc gagtcccctc caaaaaccca gccaacagag gacaacaagt tcaaattctg
     6841 tgtgagctgt gcccgtctgg ctgagatgag gcaaaaagaa atccccaggg tcctggagca
     6901 gctcgaggac ctggatagcc gggtcctcta ctactcagcc accaagaacg gcatcctgta
     6961 ccgagttggt gatggtgtgt acctgccccc tgaggccttc acgttcaaca tcaagctgtc
     7021 cagtcccgtg aaacgcccac ggaaggagcc cgtggatgag gacctgtacc cagagcacta
     7081 ccggaaatac tccgactaca tcaaaggcag caacctggat gcccctgagc cctaccgaat
     7141 tggccggatc aaagagatct tctgtcccaa gaagagcaac ggcaggccca atgagactga
     7201 catcaaaatc cgggtcaaca agttctacag gcctgagaac acccacaagt ccactccagc
     7261 gagctaccac gcagacatca acctgctcta ctggagcgac gaggaggccg tggtggactt
     7321 caaggctgtg cagggccgct gcaccgtgga gtatggggag gacctgcccg agtgcgtcca
     7381 ggtgtactcc atgggcggcc ccaaccgctt ctacttcctc gaggcctata atgcaaagag
     7441 caaaagcttt gaagatcctc ccaaccatgc ccgtagccct ggaaacaaag ggaagggcaa
     7501 gggaaaaggg aagggcaagc ccaagtccca agcctgtgag ccgagcgagc cagagataga
     7561 gatcaagctg cccaagctgc ggaccctgga tgtgttttct ggctgcgggg ggttgtcgga
     7621 gggattccac caagcaggca tctctgacac gctgtgggcc atcgagatgt gggaccctgc
     7681 ggcccaggcg ttccggctga acaaccccgg ctccacagtg ttcacagagg actgcaacat
     7741 cctgctgaag ctggtcatgg ctggggagac caccaactcc cgcggccagc ggctgcccca
     7801 gaagggagac gtggagatgc tgtgcggcgg gccgccctgc cagggcttca gcggcatgaa
     7861 ccgcttcaat tcgcgcacct actccaagtt caaaaactct ctggtggttt ccttcctcag
     7921 ctactgcgac tactaccggc cccggttctt cctcctggag aatgtcagga actttgtctc
     7981 cttcaagcgc tccatggtcc tgaagctcac cctccgctgc ctggtccgca tgggctatca
     8041 gtgcaccttc ggcgtgctgc aggccggtca gtacggcgtg gcccagacta ggaggcgggc
     8101 catcatcctg gccgcggccc ctggagagaa gctccctctg ttcccggagc cactgcacgt
     8161 gtttgctccc cgggcctgcc agctgagcgt ggtggtggat gacaagaagt ttgtgagcaa
     8221 cataaccagg ttgagctcgg gtcctttccg gaccatcacg gtgcgagaca cgatgtccga
     8281 cctgccggag gtgcggaatg gagcctcggc actggagatc tcctacaacg gggagcctca
     8341 gtcctggttc cagaggcagc tccggggcgc acagtaccag cccatcctca gggaccacat
     8401 ctgtaaggac atgagtgcat tggtggctgc ccgcatgcgg cacatcccct tggccccagg
     8461 gtcagactgg cgcgatctgc ccaacatcga ggtgcggctc tcagacggca ccatggccag
     8521 gaagctgcgg tatacccacc atgacaggaa gaacggccgc agcagctctg gggccctccg
     8581 tggggtctgc tcctgcgtgg aagccggcaa agcctgcgac cccgcagcca ggcagttcaa
     8641 caccctcatc ccctggtgcc tgccccacac cgggaaccgg cacaaccact gggctggcct
     8701 ctatggaagg ctcgagtggg acggcttctt cagcacaacc gtcaccaacc ccgagcccat
     8761 gggcaagcag ggccgcgtgc tccacccaga gcagcaccgt gtggtgagcg tgcgggagtg
     8821 tgcccgctcc cagggcttcc ctgacaccta ccggctcttc ggcaacatcc tggacaagca
     8881 ccggcaggtg ggcaatgccg tgccaccgcc cctggccaaa gccattggct tggagatcaa
     8941 gctttgtatg ttggccaaag cccgagagag tgcctcagct aaaataaagg aggaggaagc
     9001 tgctaaggac tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     9061 tatatcttgt ggaaaggacg aacctatgca tggtgtgctt catttacgcg ttaagtcgac
     9121 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9181 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9241 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9301 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9361 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9421 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9481 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     9541 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9601 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9661 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     9721 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9781 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    10021 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    10081 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10141 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10201 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    10261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10381 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    10441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10561 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    10621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    10681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    10741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    10801 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10921 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    11041 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    11101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    11161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    11221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    11281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    11341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    11401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    11461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    11521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    11581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    11641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    11701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    11761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    11821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    11881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    11941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    12001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    12061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    12121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    12181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    12241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    12301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    12361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    12421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    12481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    12541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    12601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    12661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    12721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    12781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    12841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    12901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    12961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    13021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    13081 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    13141 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    13201 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    13261 aagc
//
