LOCUS       sequence-399050-       16033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0757
FEATURES             Location/Qualifiers
     source          1..16033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11890..12478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11943..11963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12361..12372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12550..12783
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12861..12982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12898..12917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12952..12971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13022..13157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13177..13196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13178..13196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13206..13223)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13206..13222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13215..13237)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13364..13819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13451..13470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13661..13682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13845..13949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13950..14018,14019..14810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14168..14187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14981..15569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15470..15489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15723..15740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15857..15878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15893..15898,15899..15916,15917..15923)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15931..15947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15936..15958
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15955..15971
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15955..15971
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15990..16010
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15992..16010
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccg ccgctcctgg cgcccctgct
     4141 ctgcctggcg ctgctgcccg cgctcgccgc acgaggcccg cgatgctccc agcccggtga
     4201 gacctgcctg aatggcggga agtgtgaagc ggccaatggc acggaggcct gcgtctgtgg
     4261 cggggccttc gtgggcccgc gatgccagga ccccaacccg tgcctcagca ccccctgcaa
     4321 gaacgccggg acatgccacg tggtggaccg cagaggcgtg gcagactatg cctgcagctg
     4381 tgccctgggc ttctctgggc ccctctgcct gacacccctg gacaatgcct gcctcaccaa
     4441 cccctgccgc aacgggggca cctgcgacct gctcacgctg acggagtaca agtgccgctg
     4501 cccgcccggc tggtcaggga aatcgtgcca gcaggctgac ccgtgcgcct ccaacccctg
     4561 cgccaacggt ggccagtgcc tgcccttcga ggcctcctac atctgccact gcccacccag
     4621 cttccatggc cccacctgcc ggcaggatgt caacgagtgt ggccagaagc ccgggctttg
     4681 ccgccacgga ggcacctgcc acaacgaggt cggctcctac cgctgcgtct gccgcgccac
     4741 ccacactggc cccaactgcg agcggcccta cgtgccctgc agcccctcgc cctgccagaa
     4801 cgggggcacc tgccgcccca cgggcgacgt cacccacgag tgtgcctgcc tgccaggctt
     4861 caccggccag aactgtgagg aaaatatcga cgattgtcca ggaaacaact gcaagaacgg
     4921 gggtgcctgt gtggacggcg tgaacaccta caactgccgc tgcccgccag agtggacagg
     4981 tcagtactgt accgaggatg tggacgagtg ccagctgatg ccaaatgcct gccagaacgg
     5041 cgggacctgc cacaacaccc acggtggcta caactgcgtg tgtgtcaacg gctggactgg
     5101 tgaggactgc agcgagaaca ttgatgactg tgccagcgcc gcctgcttcc acggcgccac
     5161 ctgccatgac cgtgtggcct ccttctactg cgagtgtccc catggccgca caggtctgct
     5221 gtgccacctc aacgacgcat gcatcagcaa cccctgtaac gagggctcca actgcgacac
     5281 caaccctgtc aatggcaagg ccatctgcac ctgcccctcg gggtacacgg gcccggcctg
     5341 cagccaggac gtggatgagt gctcgctggg tgccaacccc tgcgagcatg cgggcaagtg
     5401 catcaacacg ctgggctcct tcgagtgcca gtgtctgcag ggctacacgg gcccccgatg
     5461 cgagatcgac gtcaacgagt gcgtctcgaa cccgtgccag aacgacgcca cctgcctgga
     5521 ccagattggg gagttccagt gcatctgcat gcccggctac gagggtgtgc actgcgaggt
     5581 caacacagac gagtgtgcca gcagcccctg cctgcacaat ggccgctgcc tggacaagat
     5641 caatgagttc cagtgcgagt gccccacggg cttcactggg catctgtgcc agtacgatgt
     5701 ggacgagtgt gccagcaccc cctgcaagaa tggtgccaag tgcctggacg gacccaacac
     5761 ttacacctgt gtgtgcacgg aagggtacac ggggacgcac tgcgaggtgg acatcgatga
     5821 gtgcgacccc gacccctgcc actacggctc ctgcaaggac ggcgtcgcca ccttcacctg
     5881 cctctgccgc ccaggctaca cgggccacca ctgcgagacc aacatcaacg agtgctccag
     5941 ccagccctgc cgccacgggg gcacctgcca ggaccgcgac aacgcctacc tctgcttctg
     6001 cctgaagggg accacaggac ccaactgcga gatcaacctg gatgactgtg ccagcagccc
     6061 ctgcgactcg ggcacctgtc tggacaagat cgatggctac gagtgtgcct gtgagccggg
     6121 ctacacaggg agcatgtgta acatcaacat cgatgagtgt gcgggcaacc cctgccacaa
     6181 cgggggcacc tgcgaggacg gcatcaatgg cttcacctgc cgctgccccg agggctacca
     6241 cgaccccacc tgcctgtctg aggtcaatga gtgcaacagc aacccctgcg tccacggggc
     6301 ctgccgggac agcctcaacg ggtacaagtg cgactgtgac cctgggtgga gtgggaccaa
     6361 ctgtgacatc aacaacaatg agtgtgaatc caacccttgt gtcaacggcg gcacctgcaa
     6421 agacatgacc agtggctacg tgtgcacctg ccgggagggc ttcagcggtc ccaactgcca
     6481 gaccaacatc aacgagtgtg cgtccaaccc atgtctgaac cagggcacgt gtattgacga
     6541 cgttgccggg tacaagtgca actgcctgct gccctacaca ggtgccacgt gtgaggtggt
     6601 gctggccccg tgtgccccca gcccctgcag aaacggcggg gagtgcaggc aatccgagga
     6661 ctatgagagc ttctcctgtg tctgccccac gggctggcaa gggcagacct gtgaggtcga
     6721 catcaacgag tgcgttctga gcccgtgccg gcacggcgca tcctgccaga acacccacgg
     6781 cggctaccgc tgccactgcc aggccggcta cagtgggcgc aactgcgaga ccgacatcga
     6841 cgactgccgg cccaacccgt gtcacaacgg gggctcctgc acagacggca tcaacacggc
     6901 cttctgcgac tgcctgcccg gcttccgggg cactttctgt gaggaggaca tcaacgagtg
     6961 tgccagtgac ccctgccgca acggggccaa ctgcacggac tgcgtggaca gctacacgtg
     7021 cacctgcccc gcaggcttca gcgggatcca ctgtgagaac aacacgcctg actgcacaga
     7081 gagctcctgc ttcaacggtg gcacctgcgt ggacggcatc aactcgttca cctgcctgtg
     7141 tccacccggc ttcacgggca gctactgcca gcacgatgtc aatgagtgcg actcacagcc
     7201 ctgcctgcat ggcggcacct gtcaggacgg ctgcggctcc tacaggtgca cctgccccca
     7261 gggctacact ggccccaact gccagaacct tgtgcactgg tgtgactcct cgccctgcaa
     7321 gaacggcggc aaatgctggc agacccacac ccagtaccgc tgcgagtgcc ccagcggctg
     7381 gaccggcctt tactgcgacg tgcccagcgt gtcctgtgag gtggctgcgc agcgacaagg
     7441 tgttgacgtt gcccgcctgt gccagcatgg agggctctgt gtggacgcgg gcaacacgca
     7501 ccactgccgc tgccaggcgg gctacacagg cagctactgt gaggacctgg tggacgagtg
     7561 ctcacccagc ccctgccaga acggggccac ctgcacggac tacctgggcg gctactcctg
     7621 caagtgcgtg gccggctacc acggggtgaa ctgctctgag gagatcgacg agtgcctctc
     7681 ccacccctgc cagaacgggg gcacctgcct cgacctcccc aacacctaca agtgctcctg
     7741 cccacggggc actcagggtg tgcactgtga gatcaacgtg gacgactgca atccccccgt
     7801 tgaccccgtg tcccggagcc ccaagtgctt taacaacggc acctgcgtgg accaggtggg
     7861 cggctacagc tgcacctgcc cgccgggctt cgtgggtgag cgctgtgagg gggatgtcaa
     7921 cgagtgcctg tccaatccct gcgacgcccg tggcacccag aactgcgtgc agcgcgtcaa
     7981 tgacttccac tgcgagtgcc gtgctggtca caccgggcgc cgctgcgagt ccgtcatcaa
     8041 tggctgcaaa ggcaagccct gcaagaatgg gggcacctgc gccgtggcct ccaacaccgc
     8101 ccgcgggttc atctgcaagt gccctgcggg cttcgagggc gccacgtgtg agaatgacgc
     8161 tcgtacctgc ggcagcctgc gctgcctcaa cggcggcaca tgcatctccg gcccgcgcag
     8221 ccccacctgc ctgtgcctgg gccccttcac gggccccgaa tgccagttcc cggccagcag
     8281 cccctgcctg ggcggcaacc cctgctacaa ccaggggacc tgtgagccca catccgagag
     8341 ccccttctac cgttgcctgt gccccgccaa attcaacggg ctcttgtgcc acatcctgga
     8401 ctacagcttc gggggtgggg ccgggcgcga catccccccg ccgctgatcg aggaggcgtg
     8461 cgagctgccc gagtgccagg aggacgcggg caacaaggtc tgcagcctgc agtgcaacaa
     8521 ccacgcgtgc ggctgggacg gcggtgactg ctccctcaac ttcaatgacc cctggaagaa
     8581 ctgcacgcag tctctgcagt gctggaagta cttcagtgac ggccactgtg acagccagtg
     8641 caactcagcc ggctgcctct tcgacggctt tgactgccag cgtgcggaag gccagtgcaa
     8701 ccccctgtac gaccagtact gcaaggacca cttcagcgac gggcactgcg accagggctg
     8761 caacagcgcg gagtgcgagt gggacgggct ggactgtgcg gagcatgtac ccgagaggct
     8821 ggcggccggc acgctggtgg tggtggtgct gatgccgccg gagcagctgc gcaacagctc
     8881 cttccacttc ctgcgggagc tcagccgcgt gctgcacacc aacgtggtct tcaagcgtga
     8941 cgcacacggc cagcagatga tcttccccta ctacggccgc gaggaggagc tgcgcaagca
     9001 ccccatcaag cgtgccgccg agggctgggc cgcacctgac gccctgctgg gccaggtgaa
     9061 ggcctcgctg ctccctggtg gcagcgaggg tgggcggcgg cggagggagc tggaccccat
     9121 ggacgtccgc ggctccatcg tctacctgga gattgacaac cggcagtgtg tgcaggcctc
     9181 ctcgcagtgc ttccagagtg ccaccgacgt ggccgcattc ctgggagcgc tcgcctcgct
     9241 gggcagcctc aacatcccct acaagatcga ggccgtgcag agtgagaccg tggagccgcc
     9301 cccgccggcg cagctgcact tcatgtacgt ggcggcggcc gcctttgtgc ttctgttctt
     9361 cgtgggctgc ggggtgctgc tgtcccgcaa gcgccggcgg cagcatggcc agctctggtt
     9421 ccctgagggc ttcaaagtgt ctgaggccag caagaagaag cggcgggagc ccctcggcga
     9481 ggactccgtg ggcctcaagc ccctgaagaa cgcttcagac ggtgccctca tggacgacaa
     9541 ccagaatgag tggggggacg aggacctgga gaccaagaag ttccggttcg aggagcccgt
     9601 ggttctgcct gacctggacg accagacaga ccaccggcag tggactcagc agcacctgga
     9661 tgccgctgac ctgcgcatgt ctgccatggc ccccacaccg ccccagggtg aggttgacgc
     9721 cgactgcatg gacgtcaatg tccgcgggcc tgatggcttc accccgctca tgatcgcctc
     9781 ctgcagcggg ggcggcctgg agacgggcaa cagcgaggaa gaggaggacg cgccggccgt
     9841 catctccgac ttcatctacc agggcgccag cctgcacaac cagacagacc gcacgggcga
     9901 gaccgccttg cacctggccg cccgctactc acgctctgat gccgccaagc gcctgctgga
     9961 ggccagcgca gatgccaaca tccaggacaa catgggccgc accccgctgc atgcggctgt
    10021 gtctgccgac gcacaaggtg tcttccagat cctgatccgg aaccgagcca cagacctgga
    10081 tgcccgcatg catgatggca cgacgccact gatcctggct gcccgcctgg ccgtggaggg
    10141 catgctggag gacctcatca actcacacgc cgacgtcaac gccgtagatg acctgggcaa
    10201 gtccgccctg cactgggccg ccgccgtgaa caatgtggat gccgcagttg tgctcctgaa
    10261 gaacggggct aacaaagata tgcagaacaa cagggaggag acacccctgt ttctggccgc
    10321 ccgggagggc agctacgaga ccgccaaggt gctgctggac cactttgcca accgggacat
    10381 cacggatcat atggaccgcc tgccgcgcga catcgcacag gagcgcatgc atcacgacat
    10441 cgtgaggctg ctggacgagt acaacctggt gcgcagcccg cagctgcacg gagccccgct
    10501 ggggggcacg cccaccctgt cgcccccgct ctgctcgccc aacggctacc tgggcagcct
    10561 caagcccggc gtgcagggca agaaggtccg caagcccagc agcaaaggcc tggcctgtgg
    10621 aagcaaggag gccaaggacc tcaaggcacg gaggaagaag tcccaggacg gcaagggctg
    10681 cctgctggac agctccggca tgctctcgcc cgtggactcc ctggagtcac cccatggcta
    10741 cctgtcagac gtggcctcgc cgccactgct gccctccccg ttccagcagt ctccgtccgt
    10801 gcccctcaac cacctgcctg ggatgcccga cacccacctg ggcatcgggc acctgaacgt
    10861 ggcggccaag cccgagatgg cggcgctggg tgggggcggc cggctggcct ttgagactgg
    10921 cccacctcgt ctctcccacc tgcctgtggc ctctggcacc agcaccgtcc tgggctccag
    10981 cagcggaggg gccctgaatt tcactgtggg cgggtccacc agtttgaatg gtcaatgcga
    11041 gtggctgtcc cggctgcaga gcggcatggt gccgaaccaa tacaaccctc tgcgggggag
    11101 tgtggcacca ggccccctga gcacacaggc cccctccctg cagcatggca tggtaggccc
    11161 gctgcacagt agccttgctg ccagcgccct gtcccagatg atgagctacc agggcctgcc
    11221 cagcacccgg ctggccaccc agcctcacct ggtgcagacc cagcaggtgc agccacaaaa
    11281 cttacagatg cagcagcaga acctgcagcc agcaaacatc cagcagcagc aaagcctgca
    11341 gccgccacca ccaccaccac agccgcacct tggcgtgagc tcagcagcca gcggccacct
    11401 gggccggagc ttcctgagtg gagagccgag ccaggcagac gtgcagccac tgggccccag
    11461 cagcctggcg gtgcacacta ttctgcccca ggagagcccc gccctgccca cgtcgctgcc
    11521 atcctcgctg gtcccacccg tgaccgcagc ccagttcctg acgcccccct cgcagcacag
    11581 ctactcctcg cctgtggaca acacccccag ccaccagcta caggtgcctg agcacccctt
    11641 cctcaccccg tcccctgagt cccctgacca gtggtccagc tcgtccccgc attccaacgt
    11701 ctccgactgg tccgagggcg tctccagccc tcccaccagc atgcagtccc agatcgcccg
    11761 cattccggag gccttcaagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
    11821 ttggctttat atatcttgtg gaaaggacga gctccacgtg cccaccagag acccacgcgt
    11881 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    11941 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    12001 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    12061 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    12121 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    12181 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    12241 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    12301 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    12361 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    12421 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
    12481 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    12541 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    12601 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    12661 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    12721 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    12781 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    12841 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    12901 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    12961 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    13021 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    13081 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    13141 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    13201 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    13261 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    13321 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    13381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    13441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    13501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    13561 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    13621 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    13681 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    13741 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    13801 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
    13861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
    13921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
    13981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
    14041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
    14101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
    14161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
    14221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
    14281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    14341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    14401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    14461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    14521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    14581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    14641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    14701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    14761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    14821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    14881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    14941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    15001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    15061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    15121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    15181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    15241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    15301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    15361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    15421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    15481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    15541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    15601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    15661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    15721 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    15781 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    15841 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    15901 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    15961 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    16021 tggagctgca agc
//
