LOCUS       sequence-399067-       11911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0546
FEATURES             Location/Qualifiers
     source          1..11911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7768..8356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7821..7841)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8239..8250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8428..8661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8739..8860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8776..8795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8830..8849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8900..9035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9055..9074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9056..9074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9084..9101)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9084..9100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9093..9115)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9242..9697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9329..9348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9539..9560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9723..9827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9828..9896,9897..10688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10046..10065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10859..11447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11348..11367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11601..11618
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11735..11756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11771..11776,11777..11794,11795..11801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11809..11825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11814..11836
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11833..11849
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11833..11849
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11868..11888
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11870..11888
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag acgcgggcgg cagaaaacac
     4141 ggccatcttt atgtgtaaat gttgtaacct cttctcacca aatcagtcgg aactcctctc
     4201 ccacgtttca gagaagcaca tggaagaagg ggttaatgtt gatgagatta ttattcccct
     4261 taggcctctg agtacacctg aaccccccaa ctcaagcaaa accggagatg agtttttggt
     4321 catgaagagg aagagaggca ggcctaaggg gtccacgaag aagtccagca cagaagagga
     4381 gctggcagaa aacatcgtga gtccgactga ggacagcccg ctggctccgg aggaagggaa
     4441 cagcctgcct ccaagcagct tggagtgtag caagtgctgt cggaagttct ccaacacgcg
     4501 ccagctgcgg aagcacatct gcattatcgt gctgaatttg ggtgaggagg aaggagaagc
     4561 aggtgctacc aagattgtgc cagtggaggc tgggccccct gaaacaggag ctacaaattc
     4621 tgagaccact tcagcagacc tggtgcctcg gagaggctac caggaatacg ccattcagca
     4681 gacaccttat gagcaaccaa tgaagtcaag caggctaggt cccactcagc tcaaaatctt
     4741 cacttgtgaa tactgcaaca aggtcttcaa gttcaagcac tcgctgcagg cccacctgag
     4801 gatccacacc aatgaaaagc catacaagtg cccccagtgc agctatgcca gtgccatcaa
     4861 ggccaacctc aatgtgcacc tgcgcaagca cactggagag aagttcgcct gcgactattg
     4921 ctcgttcacc tgcctgagca agggccacct caaggtgcac atcgagcgag tgcacaagaa
     4981 gatcaagcag cactgccgct tctgcaagaa gaagtactct gacgtcaaga acctcatcaa
     5041 gcacatccga gacgcgcatg acccacagga caagaaggtc aaagaggcct tggacgagct
     5101 ctgcctgatg acgagggagg gcaagcggca gctgctctat gactgccaca tctgtgagcg
     5161 caagttcaag aacgagctgg accgtgaccg ccatatgctg gtccacggag acaagtggcc
     5221 ttttgcctgt gagctctgtg gccatggggc caccaagtac caggcgctgg aactgcatgt
     5281 caggaagcac cccttcgtgt acgtctgtgc cgtctgccgc aagaagttcg tcagctccat
     5341 caggctgcgc acccacatca aagaggtgca cggggctgcc caggaggcct tggtcttcac
     5401 cagttccatc aaccagagct tctgcctcct ggaacctggt ggggacatcc agcaagaagc
     5461 tctgggggac cagctacagc tggtggaaga ggagtttgcc ctccagggcg tgaatgcact
     5521 caaggaagag gcctgtcctg gggacactca gctggaggag ggccggaagg agccggaggc
     5581 ccctggggaa atgcctgccc cagctgtgca cctggcctcc ccgcaggccg aaagcacagc
     5641 cctgccaccc tgtgagctgg aaaccaccgt ggtctcctcc tcagacctgc attctcaaga
     5701 ggtggtttca gatgattttt tgttgaaaaa tgatacctcc tccgcagagg ctcatgctgc
     5761 tcctgagaag cccccagaca tgcagcacag aagctcagtc cagacgcaag gtgaagtgat
     5821 cacactactg ctgtccaagg cccagagtgc tgggtcagat caggaaagcc atggcgccca
     5881 gagcccccta ggggaagggc agaacatggc tgtgctttca gctggtgacc cagatcccag
     5941 caggtgtctc aggtcaaacc cagctgaggc ctcagacctc ctccctccag tagctggtgg
     6001 tggggacacc atcacacatc agcctgactc ttgcaaagct gcccctgagc accggtcagg
     6061 catcaccgct ttcatgaagg tcctgaacag tttacagaag aagcaaatga acaccagctt
     6121 gtgtgagcgg atccggaagg tttatggaga cctggagtgt gaatactgtg gcaaactttt
     6181 ttggtaccaa gtgcattttg atatgcatgt ccgcacccac acccgggaac atctgtatta
     6241 ttgctctcag tgtcattatt cttccatcac caaaaactgc cttaaacgcc acgtaattca
     6301 gaaacacagt aacatcttgc tgaagtgtcc caccgatggc tgtgactact caactccaga
     6361 taaatataag ctacaggcac atcttaaagt tcacacagca ctggacaaaa ggagttattc
     6421 ttgtcctgtt tgtgaaaagt ctttttcaga ggatcgattg ataaagtcac atatcaagac
     6481 caaccatcct gaggtctcca tgagcaccat ttctgaggtt ctcgggagga gggttcagct
     6541 gaaagggcta attggaaaga gagccatgaa atgcccatat tgtgactttt atttcatgaa
     6601 gaatggctca gaccttcagc gtcatatttg ggctcatgaa ggtgtgaagc ccttcaagtg
     6661 ttctttgtgt gagtatgcaa ctcgtagcaa gagtaacctc aaggctcata tgaatcgtca
     6721 cagcactgag aaaacccacc tatgtgacat gtgtggcaag aaattcaaat caaaagggac
     6781 actgaaaagt cacaaactcc ttcacactgc agatgggaag cagtttaagt gcacggtgtg
     6841 tgactacaca gcggcccaga agccacagct gctgcggcac atggaacagc atgtctcctt
     6901 caagcctttc cgctgtgccc attgccatta ctcctgcaac atatctggct ctctgaagcg
     6961 gcactacaac aggaagcacc ctaatgagga gtatgccaac gtgggcaccg gggagctggc
     7021 agcggaggtg ctcatccagc aaggtggttt gaagtgtcct gtttgcagct ttgtatatgg
     7081 caccaaatgg gagttcaata ggcacttgaa gaacaaacat ggcttgaagg tggtggaaat
     7141 tgatggagac cccaagtggg agacagcaac agaagctcct gaggagccct ccacccagta
     7201 tctccacatc acagaggccg aagaagacgt tcaagggaca caggcagcgg tggccgcgct
     7261 ccaggacctg agatacacct ctgagagtgg cgaccgactg gaccccacgg ccgtgaacat
     7321 cctgcagcag atcattgagc tgggcgccga gacccatgac gccactgccc ttgcctcggt
     7381 ggttgccatg gcaccaggga cggtgactgt ggttaagcag gtcaccgagg aggagcccag
     7441 ctccaaccac acggtcatga tccaggagac ggtccagcaa gcgtccgtgg agcttgccga
     7501 gcagcaccac ctggtggtgt cctccgacga cgtggagggc attgagacgg tgactgtcta
     7561 cacgcagggc ggggaggcct cggagttcat cgtctacgtg caggaggcca tgcagcctgt
     7621 ggaggagcag gctgtggagc agccggccca ggaactctag taatgaacta gtccgtaact
     7681 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgatg ccacgtcccg
     7741 cgctcatata ttacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7801 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7861 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7921 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7981 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8041 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8101 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8161 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8221 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8281 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8341 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8401 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8461 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8521 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8581 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8641 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     8701 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8881 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8941 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9001 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9061 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9121 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9241 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9661 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     9721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9841 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9901 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9961 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10021 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10081 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10141 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10201 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10261 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10321 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10381 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10441 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10501 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10561 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10621 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10681 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10741 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11281 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11461 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11521 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11581 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11641 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    11701 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11761 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11821 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    11881 actaaaggga acaaaagctg gagctgcaag c
//
