LOCUS       sequence-399075-       11803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the ZFAT transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0543
FEATURES             Location/Qualifiers
     source          1..11803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7660..8248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7713..7733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8131..8142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8320..8553
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8631..8752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8668..8687)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8722..8741
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8792..8927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8947..8966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8948..8966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8976..8993)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8976..8992)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8985..9007)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9134..9589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9221..9240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9431..9452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9615..9719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9720..9788,9789..10580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9938..9957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10751..11339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11240..11259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11493..11510
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11627..11648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11663..11668,11669..11686,11687..11693)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11701..11717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11706..11728
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11725..11741
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11725..11741
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11760..11780
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11762..11780
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtgt aaatgttgta acctcttctc
     4141 accaaatcag tcggaactcc tctcccacgt ttcagagaag cacatggaag aaggggttaa
     4201 tgttgatgag attattattc cccttaggcc tctgagtaca cctgaacccc ccaactcaag
     4261 caaaaccgga gatgagtttt tggtcatgaa gaggaagaga ggcaggccta aggggtccac
     4321 gaagaagtcc agcacagaag aggagctggc agaaaacatc gtgagtccga ctgaggacag
     4381 cccgctggct ccggaggaag ggaacagcct gcctccaagc agcttggagt gtagcaagtg
     4441 ctgtcggaag ttctccaaca cgcgccagct gcggaagcac atctgcatta tcgtgctgaa
     4501 tttgggtgag gaggaaggag aagcaggtaa cgagtctgac cttgaactag aaaagaagtg
     4561 taaggaagat gatcgggaaa aagcctcgaa aagaccacgg tcacagaaaa cagagaaagt
     4621 ccagaagatc tcaggaaagg aggccagaca gctttctggg gcgaagaaac ccatcataag
     4681 tgtggtttta actgcacacg aagcaattcc aggtgctacc aagattgtgc cagtggaggc
     4741 tgggccccct gaaacaggag ctacaaattc tgagaccact tcagcagacc tggtgcctcg
     4801 gagaggctac caggaatacg ccattcagca gacaccttat gagcaaccaa tgaagtcaag
     4861 caggctaggt cccactcagc tcaaaatctt cacttgtgaa tactgcaaca aggtcttcaa
     4921 gttcaagcac tcgctgcagg cccacctgag gatccacacc aatgaaaagc catacaagtg
     4981 cccccagtgc agctatgcca gtgccatcaa ggccaacctc aatgtgcacc tgcgcaagca
     5041 cactggagag aagttcgcct gcgactattg ctcgttcacc tgcctgagca agggccacct
     5101 caaggtgcac atcgagcgag tgcacaagaa gatcaagcag cactgccgct tctgcaagaa
     5161 gaagtactct gacgtcaaga acctcatcaa gcacatccga gacgcgcatg acccacagga
     5221 caagaaggtc aaagaggcct tggacgagct ctgcctgatg acgagggagg gcaagcggca
     5281 gctgctctat gactgccaca tctgtgagcg caagttcaag aacgagctgg accgtgaccg
     5341 ccatatgctg gtccacggag acaagtggcc ttttgcctgt gagctctgtg gccatggggc
     5401 caccaagtac caggcgctgg aactgcatgt caggaagcac cccttcgtgt acgtctgtgc
     5461 cgtctgccgc aagaagttcg tcagctccat caggctgcgc acccacatca aagaggtgca
     5521 cggggctgcc caggaggcct tggtcttcac cagttccatc aaccagagct tctgcctcct
     5581 ggaacctggt ggggacatcc agcaagaagc tctgggggac cagctacagc tggtggaaga
     5641 ggagtttgcc ctccagggcg tgaatgcact caaggaagag gcctgtcctg gggacactca
     5701 gctggaggag ggccggaagg agccggaggc ccctggggaa atgcctgccc cagctgtgca
     5761 cctggcctcc ccgcaggccg aaagcacagc cctgccaccc tgtgagctgg aaaccaccgt
     5821 ggtctcctcc tcagacctgc attctcaaga ggtggtttca gatgattttt tgttgaaaaa
     5881 tgatacctcc tccgcagagg ctcatgctgc tcctgagaag cccccagaca tgcagcacag
     5941 aagctcagtc cagacgcaag gtgaagtgat cacactactg ctgtccaagg cccagagtgc
     6001 tgggtcagat caggaaagcc atggcgccca gagcccccta ggggaagggc agaacatggc
     6061 tgtgctttca gctggtgacc cagatcccag caggtgtctc aggtcaaacc cagctgaggc
     6121 ctcagacctc ctccctccag tagctggtgg tggggacacc atcacacatc agcctgactc
     6181 ttgcaaagct gcccctgagc accggtcagg catcaccgct ttcatgaagg tcctgaacag
     6241 tttacagaag aagcaaatga acaccagctt gtgtgagcgg atccggaagg tttatggaga
     6301 cctggagtgt gaatactgtg gcaaactttt ttggtaccaa gtgcattttg atatgcatgt
     6361 ccgcacccac acccgggaac atctgtatta ttgctctcag tgtcattatt cttccatcac
     6421 caaaaactgc cttaaacgcc acgtaattca gaaacacagt aacatcttgc tgaagtgtcc
     6481 caccgatggc tgtgactact caactccaga taaatataag ctacaggcac atcttaaagt
     6541 tcacacagca ctggacaaaa ggagttattc ttgtcctgtt tgtgaaaagt ctttttcaga
     6601 ggatcgattg ataaagtcac atatcaagac caaccatcct gaggtctcca tgagcaccat
     6661 ttctgaggtt ctcgggagga gggttcagct gaaagggcta attggaaaga gagccatgaa
     6721 atgcccatat tgtgactttt atttcatgaa gaatggctca gaccttcagc gtcatatttg
     6781 ggctcatgaa ggtgtgaagc ccttcaagtg ttctttgtgt gagtatgcaa ctcgtagcaa
     6841 gagtaacctc aaggctcata tgaatcgtca cagcactgag aaaacccacc tatgtgacat
     6901 gtgtggcaag aaattcaaat caaaagggac actgaaaagt cacaaactcc ttcacactgc
     6961 agatgggaag cagtttaagt gcacggtgtg tgactacaca gcggcccaga agccacagct
     7021 gctgcggcac atggaacagc atgtctcctt caagcctttc cgctgtgccc attgccatta
     7081 ctcctgcaac atatctggct ctctgaagcg gcactacaac aggaagcacc ctaatgagga
     7141 gtatgccaac gtgggcaccg gggagctggc agcggaggtg ctcatccagc aaggtggttt
     7201 gaagtgtcct gtttgcagct ttgtatatgg caccaaatgg gagttcaata ggcacttgaa
     7261 gaacaaacat ggcttgaagg tggtggaaat tgatggagac cccaagtggg aggtcaccga
     7321 ggaggagccc agctccaacc acacggtcat gatccaggag acggtccagc aagcgtccgt
     7381 ggagcttgcc gagcagcacc acctggtggt gtcctccgac gacgtggagg gcattgagac
     7441 ggtgactgtc tacacgcagg gcggggaggc ctcggagttc atcgtctacg tgcaggaggc
     7501 catgcagcct gtggaggagc aggctgtgga gcagccggcc caggaactct agtaatgaac
     7561 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     7621 taatgagtaa atttactcag ctcgacgcgt taagtcgaca atcaacctct ggattacaaa
     7681 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7741 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7801 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7861 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7921 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7981 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8041 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8101 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8161 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8221 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     8281 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8341 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8401 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8461 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8521 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     8581 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8761 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8821 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8881 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8941 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9001 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9061 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9121 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9421 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9481 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9541 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9601 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9661 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9721 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9781 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9841 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9901 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9961 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10021 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10081 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10141 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10201 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10261 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10321 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10381 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10441 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10501 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10561 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10621 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10861 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11401 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11461 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11521 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11581 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11641 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11701 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11761 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
