LOCUS       sequence-399078-        2435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sulfydryl oxidase (Erv1p) after phosphate depletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Gent-yibDp-Erv1p
FEATURES             Location/Qualifiers
     source          1..2435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1755..1782
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1788..1878
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttgatatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga gattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgt gcgtaattgt
     1021 gctgatctct tatatagctg ctctcattat ctctctaccc tgaagtgact ctctcacctg
     1081 taaaaataat atctcacagg cttaatagtt tcttaataca aagcctgtaa aacgtcagga
     1141 taacttctgt gtaggaggat aatctatgaa ggcaattgat aagatgaccg acaatcctcc
     1201 tcaggaaggc ttgtccgggc gcaaaatcat ctatgatgag gacgggaagc cgtgccgttc
     1261 ctgcaatact ttgctggact tccaatacgt cacgggtaaa atctcgaatg gattgaagaa
     1321 tttgtcttca aatggaaaac tggcagggac tggggctctt acgggggagg catcggaact
     1381 tatgcctggt tcccgcactt atcgcaaagt agatccacct gatgttgagc agcttggccg
     1441 ttcttcgtgg accttgttgc actctgtcgc cgcgtcgtat ccagctcagc ctacggacca
     1501 acagaagggc gagatgaaac agttcctgaa catcttctcg cacatttacc cttgcaactg
     1561 gtgcgccaag gacttcgaga agtacattcg cgagaatgcg ccgcaagtag aatcccgcga
     1621 agaattggga cgttggatgt gtgaagcaca taataaggtg aataagaagt tacgcaaacc
     1681 aaagttcgac tgtaatttct gggaaaaacg ctggaaagac gggtgggacg agtaatgagg
     1741 atccgagtca cactggctca ccttcgggtg ggcctttctg cgtttattga tcggcacgta
     1801 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     1861 tcgagatttt caggagctgg aaggaagtaa aaatgttgcg tagctctaac gatgtgacgc
     1921 aacaaggttc gcgtccaaag acaaaattgg gaggcagtag catggggatc attcgcactt
     1981 gtcgcctggg gccagaccag gtgaagtcaa tgcgtgcggc tctggactta ttcgggcgcg
     2041 aatttggaga tgtagccact tactcacagc accaaccgga cagtgattac ttggggaatt
     2101 tacttcgcag taaaactttt atcgctttgg ccgctttcga ccaggaggct gtagtaggtg
     2161 cgttggcagc ctatgttctt cctaaattcg agcaaccgcg tagcgaaatt tacatctatg
     2221 atcttgcagt ctccggcgaa catcgccgtc aggggatcgc cacagcttta atcaaccttt
     2281 tgaagcatga ggctaatgca cttggagcgt acgtgattta tgtgcaggct gactacggtg
     2341 atgatcctgc agtcgctctg tacaccaaac tgggtatccg cgaggaggtc atgcactttg
     2401 atattgaccc gtctacggct acctaataga agctt
//
