LOCUS       sequence-39909-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Cowley SC, Av-Gay Y
  TITLE     Monitoring promoter activity and protein localization in 
            Mycobacterium spp. using green fluorescent protein.
  JOURNAL   Gene. 2001 Feb 21;264(2):225-31.
  PUBMED    11250077
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-02-21"; volume: "264(2)"; pages: "225-31"
COMMENT     Sequence Label: pSC300
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..58
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(148..176)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgcgtgggag gcacccgcgc gaaaagaaac atcggacgat catttcatcg aaggaaggaa
       61 tgccgtggga tcccccgggc tgcaggaatt cgatatcaag ctgtaccggt agaaaaaatg
      121 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      181 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      241 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      301 actactttga cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
      361 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      421 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      481 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      541 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      601 aaagttaact tcaaaattag acacaacatt gaagatg
//
