LOCUS       sequence-399090-        9134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; 
            doesn't bind NHERF1/2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E,
            Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou 
            K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch 
            A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman 
            JC, Zanivan S, Blyth K, Bryant DM
  TITLE     Spatial regulation of the glycocalyx component podocalyxin is a 
            switch for prometastatic function.
  JOURNAL   Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub
            2023 Feb 3.
  PUBMED    36735782
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-3"; volume: "9(5):eabq1858. doi"; pages: " 
            10.1126/sciadv.abq1858. Epub 2023 Feb 3"
COMMENT     Sequence Label: pQCXIZ GFP PODXL delta PBM
FEATURES             Location/Qualifiers
     source          1..9134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2328..2330,2331..2333,2334..3044)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2328..2330,2331..2333,2334..3044)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2373..2394)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2634..2653)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2981..3002
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4407..4418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4770..5232
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4826..4843)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5053..5072
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5246..5620
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5840..6265
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6368..6390)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6526..6546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6531..6860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6711..6846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6882..6901
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6982..6999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7153..7741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7233..7252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7912..8703,8704..8772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8535..8554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8773..8877)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8945..8963
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9063..9134,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtgccac catgcgctgc gcgctggcgc
     2281 tctcggcgct gctgctactg ttgtcaacgc cgccgctgct gccgtcgatg gtgagcaagg
     2341 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2401 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2461 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2521 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2581 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2641 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2701 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2761 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2821 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2881 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2941 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3001 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtcgccg tcgccgtcgc
     3061 cgtcgccctc ccagaatgca acccagacta ctacggactc atctaacaaa acagcaccga
     3121 ctccagcatc cagtgtcacc atcatggcta cagatacagc ccagcagagc acagtcccca
     3181 cttccaaggc caacgaaatc ttggcctcgg tcaaggcgac cacccttggt gtatccagtg
     3241 actcaccggg gactacaacc ctggctcagc aagtctcagg cccagtcaac actaccgtgg
     3301 ctagaggagg cggctcaggc aaccctacta ccaccatcga gagccccaag agcacaaaaa
     3361 gtgcagacac cactacagtt gcaacctcca cagccacagc taaacctaac accacaagca
     3421 gccagaatgg agcagaagat acaacaaact ctggggggaa aagcagccac agtgtgacca
     3481 cagacctcac atccactaag gcagaacatc tgacgacccc tcaccctaca agtccactta
     3541 gcccccgaca acccacttcg acgcatcctg tggccacccc aacaagctcg ggacatgacc
     3601 atcttatgaa aatttcaagc agttcaagca ctgtggctat ccctggctac accttcacaa
     3661 gcccggggat gaccaccacc ctactagaga cagtgtttca ccatgtcagc caggctggtc
     3721 ttgaactcct gacctcgggt gatctgccca ccttggcctc ccaaagtgct gggattacag
     3781 cgtcatcggt tatctcgcaa agaactcaac agacctccag tcagatgcca gccagctcta
     3841 cggccccttc ctcccaggag acagtgcagc ccacgagccc ggcaacggca ttgagaacac
     3901 ctaccctgcc agagaccatg agctccagcc ccacagcagc atcaactacc caccgatacc
     3961 ccaaaacacc ttctcccact gtggctcatg agagtaactg ggcaaagtgt gaggatcttg
     4021 agacacagac acagagtgag aagcagctcg tcctgaacct cacaggaaac accctctgtg
     4081 cagggggcgc ttcggatgag aaattgatct cactgatatg ccgagcagtc aaagccacct
     4141 tcaacccggc ccaagataag tgcggcatac ggctggcatc tgttccagga agtcagaccg
     4201 tggtcgtcaa agaaatcact attcacacta agctccctgc caaggatgtg tacgagcggc
     4261 tgaaggacaa atgggatgaa ctaaaggagg caggggtcag tgacatgaag ctaggggacc
     4321 aggggccacc ggaggaggcc gaggaccgct tcagcatgcc cctcatcatc accatcgtct
     4381 gcatggcatc attcctgctc ctcgtggcgg ccctctatgg ctgctgccac cagcgcctct
     4441 cccagaggaa ggaccagcag cggctaacag aggagctgca gacagtggag aatggttacc
     4501 atgacaaccc aacactggaa gtgatggaga cctcttctga gatgcaggag aagaaggtgg
     4561 tcagcctcaa cggggagctg ggggacagct ggatcgtccc tctggacaac ctgaccaagg
     4621 acgacctgga tgaggaggaa tagggayccg gaattccscc ccycncccyc ccccccccct
     4681 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     4741 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     4801 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     4861 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     4921 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4981 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     5041 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     5101 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     5161 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     5221 acacgatgat aagcttgcca caaccatggc caagttgacc agtgccgttc cggtgctcac
     5281 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     5341 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     5401 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     5461 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     5521 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     5581 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgacccgg acgggacgct
     5641 cctgcgcctg atacagaacg aattgcttgc aggcatctca tgagtgtgtc ttcccgtttt
     5701 ccgcctgagg tcactgcgtg gatggagcat cagtggtcca ggctctagtt ttgactcaac
     5761 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
     5821 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     5881 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     5941 ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc
     6001 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     6061 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     6121 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     6181 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     6241 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     6301 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg
     6361 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6421 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     6481 ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt
     6541 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6601 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6661 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6781 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6841 gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag agcagattgt
     6901 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6961 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7141 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7561 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8221 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8521 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8881 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     8941 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc cattgcatac
     9001 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
     9061 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     9121 cccatatatg gagt
//
