LOCUS       sequence-399099-       10860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; 
            doesn't bind NHERF1/2) fused to EZRIN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Roman-Fernandez A, Mansour MA, Kugeratski FG, Anand J, Sandilands E,
            Galbraith L, Rakovic K, Freckmann EC, Cumming EM, Park J, Nikolatou 
            K, Lilla S, Shaw R, Strachan D, Mason S, Patel R, McGarry L, Katoch 
            A, Campbell KJ, Nixon C, Miller CJ, Leung HY, Le Quesne J, Norman 
            JC, Zanivan S, Blyth K, Bryant DM
  TITLE     Spatial regulation of the glycocalyx component podocalyxin is a 
            switch for prometastatic function.
  JOURNAL   Sci Adv. 2023 Feb 3;9(5):eabq1858. doi: 10.1126/sciadv.abq1858. Epub
            2023 Feb 3.
  PUBMED    36735782
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-3"; volume: "9(5):eabq1858. doi"; pages: " 
            10.1126/sciadv.abq1858. Epub 2023 Feb 3"
COMMENT     Sequence Label: pQCXIZ GFP PODXL delta PBM + Ezrin
FEATURES             Location/Qualifiers
     source          1..10860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2322..2324,2325..2327,2328..3038)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2322..2324,2325..2327,2328..3038)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2367..2388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2628..2647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2975..2996
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4401..4412)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6496..6958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6552..6569)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6779..6798
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6972..7346
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             7566..7991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(8094..8116)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8252..8272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8257..8586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8437..8572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8608..8627
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8708..8725)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8879..9467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8959..8978)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9638..10429,10430..10498))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10261..10280
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10499..10603)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10671..10689
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10789..10860,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgcg ccaccatgcg ctgcgcgctg gcgctctcgg
     2281 cgctgctgct actgttgtca acgccgccgc tgctgccgtc gatggtgagc aagggcgagg
     2341 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2401 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2461 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2521 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2581 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2641 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2701 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2761 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2821 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2881 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2941 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3001 ccgccgggat cactctcggc atggacgagc tgtacaagtc gccgtcgccg tcgccgtcgc
     3061 cctcccagaa tgcaacccag actactacgg actcatctaa caaaacagca ccgactccag
     3121 catccagtgt caccatcatg gctacagata cagcccagca gagcacagtc cccacttcca
     3181 aggccaacga aatcttggcc tcggtcaagg cgaccaccct tggtgtatcc agtgactcac
     3241 cggggactac aaccctggct cagcaagtct caggcccagt caacactacc gtggctagag
     3301 gaggcggctc aggcaaccct actaccacca tcgagagccc caagagcaca aaaagtgcag
     3361 acaccactac agttgcaacc tccacagcca cagctaaacc taacaccaca agcagccaga
     3421 atggagcaga agatacaaca aactctgggg ggaaaagcag ccacagtgtg accacagacc
     3481 tcacatccac taaggcagaa catctgacga cccctcaccc tacaagtcca cttagccccc
     3541 gacaacccac ttcgacgcat cctgtggcca ccccaacaag ctcgggacat gaccatctta
     3601 tgaaaatttc aagcagttca agcactgtgg ctatccctgg ctacaccttc acaagcccgg
     3661 ggatgaccac caccctacta gagacagtgt ttcaccatgt cagccaggct ggtcttgaac
     3721 tcctgacctc gggtgatctg cccaccttgg cctcccaaag tgctgggatt acagcgtcat
     3781 cggttatctc gcaaagaact caacagacct ccagtcagat gccagccagc tctacggccc
     3841 cttcctccca ggagacagtg cagcccacga gcccggcaac ggcattgaga acacctaccc
     3901 tgccagagac catgagctcc agccccacag cagcatcaac tacccaccga taccccaaaa
     3961 caccttctcc cactgtggct catgagagta actgggcaaa gtgtgaggat cttgagacac
     4021 agacacagag tgagaagcag ctcgtcctga acctcacagg aaacaccctc tgtgcagggg
     4081 gcgcttcgga tgagaaattg atctcactga tatgccgagc agtcaaagcc accttcaacc
     4141 cggcccaaga taagtgcggc atacggctgg catctgttcc aggaagtcag accgtggtcg
     4201 tcaaagaaat cactattcac actaagctcc ctgccaagga tgtgtacgag cggctgaagg
     4261 acaaatggga tgaactaaag gaggcagggg tcagtgacat gaagctaggg gaccaggggc
     4321 caccggagga ggccgaggac cgcttcagca tgcccctcat catcaccatc gtctgcatgg
     4381 catcattcct gctcctcgtg gcggccctct atggctgctg ccaccagcgc ctctcccaga
     4441 ggaaggacca gcagcggcta acagaggagc tgcagacagt ggagaatggt taccatgaca
     4501 acccaacact ggaagtgatg gagacctctt ctgagatgca ggagaagaag gtggtcagcc
     4561 tcaacgggga gctgggggac agctggatcg tccctctgga caacctgacc aaggacgacc
     4621 tggatgagga ggaaatgccg aaaccaatca atgtccgagt taccaccatg gatgcagagc
     4681 tggagtttgc aatccagcca aatacaactg gaaaacagct ttttgatcag gtggtaaaga
     4741 ctatcggcct ccgggaagtg tggtactttg gcctccacta tgtggataat aaaggatttc
     4801 ctacctggct gaagctggat aagaaggtgt ctgcccagga ggtcaggaag gagaatcccc
     4861 tccagttcaa gttccgggcc aagttctacc ctgaagatgt ggctgaggag ctcatccagg
     4921 acatcaccca gaaacttttc ttcctccaag tgaaggaagg aatccttagc gatgagatct
     4981 actgcccccc tgagactgcc gtgctcttgg ggtcctacgc tgtgcaggcc aagtttgggg
     5041 actacaacaa agaagtgcac aagtctgggt acctcagctc tgagcggctg atccctcaaa
     5101 gagtgatgga ccagcacaaa cttaccaggg accagtggga ggaccggatc caggtgtggc
     5161 atgcggaaca ccgtgggatg ctcaaagata atgctatgtt ggaatacctg aagattgctc
     5221 aggacctgga aatgtatgga atcaactatt tcgagataaa aaacaagaaa ggaacagacc
     5281 tttggcttgg agttgatgcc cttggactga atatttatga gaaagatgat aagttaaccc
     5341 caaagattgg ctttccttgg agtgaaatca ggaacatctc tttcaatgac aaaaagtttg
     5401 tcattaaacc catcgacaag aaggcacctg actttgtgtt ttatgcccca cgtctgagaa
     5461 tcaacaagcg gatcctgcag ctctgcatgg gcaaccatga gttgtatatg cgccgcagga
     5521 agcctgacac catcgaggtg cagcagatga aggcccaggc ccgggaggag aagcatcaga
     5581 agcagctgga gcggcaacag ctggaaacag agaagaaaag gagagaaacc gtggagagag
     5641 agaaagagca gatgatgcgc gagaaggagg agttgatgct gcggctgcag gactatgagg
     5701 agaagacaaa gaaggcagag agagagctct cggagcagat tcagagggcc ctgcagctgg
     5761 aggaggagag gaagcgggca caggaggagg ccgagcgcct agaggctgac cgtatggctg
     5821 cactgcgggc taaggaggag ctggagagac aggcggtgga tcagataaag agccaggagc
     5881 agctggctgc ggagcttgca gaatacactg ccaagattgc cctcctggaa gaggcgcgga
     5941 ggcgcaagga ggatgaagtt gaagagtggc agcacagggc caaagaagcc caggatgacc
     6001 tggtgaagac caaggaggag ctgcacctgg tgatgacagc acccccgccc ccaccacccc
     6061 ccgtgtacga gccggtgagc taccatgtcc aggagagctt gcaggatgag ggcgcagagc
     6121 ccacgggcta cagcgcggag ctgtctagtg agggcatccg ggatgaccgc aatgaggaga
     6181 agcgcatcac tgaggcagag aagaacgagc gtgtgcagcg gcagctgctg acgctgagca
     6241 gcgagctgtc ccaggcccga gatgagaata agaggaccca caatgacatc atccacaacg
     6301 agaacatgag gcaaggccgg gacaagtaca agacgctgcg gcagatccgg cagggcaaca
     6361 ccaagcagcg catcgacgag ttcgaggccc tgtaagaatt ccccctaacg ttactggccg
     6421 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6481 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     6541 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     6601 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     6661 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     6721 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     6781 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     6841 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     6901 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     6961 ttgccacaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7021 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7081 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7141 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7201 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7261 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7321 acttcgtggc cgaggagcag gactgacacg acccggacgg gacgctcctg cgcctgatac
     7381 agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc cgttttccgc ctgaggtcac
     7441 tgcgtggatg gagcatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
     7501 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
     7561 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     7621 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     7681 ggaacagggt cgaccctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     7741 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     7801 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     7861 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     7921 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     7981 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
     8041 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     8101 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     8161 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     8221 cgtagcgata gcggagtgta gatccggctg tggaatgtgt gtcagttagg gtgtggaaag
     8281 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8341 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8401 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8461 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8521 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8581 tgcaaaaagc ttactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8641 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     8701 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8761 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8821 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8881 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8941 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9001 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9061 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9121 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9181 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9241 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9301 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9361 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9421 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9481 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9541 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9601 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9661 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9721 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9781 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9841 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9901 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     9961 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10021 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10081 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10141 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10201 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
    10261 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10321 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10381 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10441 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10501 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10561 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10621 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10681 tcacgaggcc ctttcgtctt caagaattag cttggccatt gcatacgttg tatccatatc
    10741 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
    10801 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
//
