LOCUS       sequence-399200-        9561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expressing membrane-localized Voltron2 and membrane-localized 
            CheRiff..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Park P, Wong-Campos JD, Itkis DG, Lee BH, Qi Y, Davis HC, Antin B, 
            Pasarkar A, Grimm JB, Plutkis SE, Holland KL, Paninski L, Lavis LD, 
            Cohen AE
  TITLE     Dendritic excitations govern back-propagation via a spike-rate 
            accelerometer.
  JOURNAL   Nat Commun. 2025 Feb 4;16(1):1333. doi: 10.1038/s41467-025-55819-9.
  PUBMED    39905023
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-4"; volume: "16(1):1333. doi"; pages: " 
            10.1038/s41467-025-55819-9"
COMMENT     Sequence Label: 
            PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
FEATURES             Location/Qualifiers
     source          1..9561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        223..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..802
                     /label=chicken beta-actin promoter
     intron          803..1819
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1743..1765
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1827..1846
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1908..1917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1980..2018
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             3699..3755
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3816..3854
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             3873..4817
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(4917..4919,4920..4922,4923..5633)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4962..4983)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5223..5242)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5570..5591
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5688..6276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5741..5761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6159..6170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6308..6784
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6507..6526)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   6824..6964
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6824..6953
                     /label=AAV2 ITR
     rep_origin      7039..7494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7126..7145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7336..7357
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7511..7530)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7630..7652
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7690..7708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7776..7880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7881..7949,7950..8741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8099..8118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8912..9500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9401..9420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccagtt tggttaatta actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
      541 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
      601 tattttttaa ttattttgtg cagcgatggg ggcggggggg ggggggcgcg cgccaggcgg
      661 ggcggggsgg ggsgrggggs ggggsggggs grggcggrga ggtgcggcgg cagccaatca
      721 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
      781 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
      841 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
      901 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
      961 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     1021 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     1081 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     1141 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     1201 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     1261 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     1321 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     1381 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     1441 cggcggtccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     1501 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     1561 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     1621 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     1681 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     1741 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     1801 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1861 tggcaaagaa ttgatttgat accgcgggcc cgggatctcg aggatccgcc accatgggcc
     1921 gaccccaaat actcctcttg ctggctttgt tgacccttgg actggctaac ggaacagaag
     1981 gtcccaactt ctacgttcct ttcagcaata agacaggcgt agtcagatcc ggtagtgctg
     2041 acgtggaaac cgagaccggc atgattgcac agtggattgt ctttgctatt atggctgctg
     2101 ctgctattgc ttttggagtg gctgtgcact ttcggccttc agagctgaag agcgcatact
     2161 atatcaacat tgccatctgc actatcgccg ctaccgctta ctatgcaatg gccgtgaact
     2221 accaggacct gacaatgaat ggtgaaaggc aggtggtcta cgcaagatat attaactggg
     2281 tgctgaccac accactgctc ctgctcgatc tcatcgtcat gaccaagatg ggcggagtga
     2341 tgatttcttg ggtcatcggc gcagacattt tcatgatcgt gtttggtatt ctgggcgact
     2401 tcgaggatga acacaagttc aaatgggtgt actttatcgc tggatgtgtg atgcaggcag
     2461 tcctgacata cgggatgtat aacgccactt ggaaagacga tctgaagaaa agccccgagt
     2521 accatagctc ctatgtcagt ctgctcgtct tcctgtcaat cctctgggtg ttttatcctg
     2581 tcgtgtgggc tttcgggtct ggtagtggcg tgctgtccgt cgacaatgag gccattctca
     2641 tgggaatcct ggatgtgctc gctaagccac tgtttggaat ggggtgcctc attgcccatg
     2701 agactatctt caagatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     2761 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     2821 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     2881 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     2941 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     3001 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     3061 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     3121 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     3181 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     3241 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     3301 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     3361 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     3421 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     3481 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     3541 cggacctgat cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggcgagc
     3601 caaccactaa gagcaggatc accagcgagg gcgagtacat ccccctggac cagatcgaca
     3661 tcaacgtgtt ctgctacgag aacgaggtgg gctccggagc cacgaacttc tctctgttaa
     3721 agcaagcagg agacgtggaa gaaaaccccg gtccccgacc ccaaatactc ctcttgctgg
     3781 ctttgttgac ccttggactg gctaacggaa cagaaggtcc caacttctac gttcctttca
     3841 gcaataagac aggcgtagtc agatccggta gtggcggagc tcctgctcca gacgctcaca
     3901 gcgccccacc tggaaacgat tctgccggag gcagtgagta ccatgcccca gctggatatc
     3961 aagtgaatcc accctaccac cccgtgcatg ggtatgagga acagtgcagc tccatctaca
     4021 tctactatgg ggccctgtgg gagcaggaaa cagctagggg cttccagtgg tttgccgtgt
     4081 tcctgtctgc cctgtttctg gctttctacg gctggcacgc ctataaggcc agcgtgggat
     4141 gggaggaagt gtacgtgtgc tccgtggagc tgatcaaagt gattctggag atctatttcg
     4201 agttcaccag tcctgctatg ctgttcctgt acggagggaa cattacccca tggctgagat
     4261 atgccgaatg gctgctgaca tgtcccgtga tcctgattca tctgtctaac atcaccggcc
     4321 tgagtgaggc atacaataag cggacaatgg ctctgctggt gtccgacctg ggaactattt
     4381 gcatgggagt gacagccgct ctggccactg ggtgggtgaa gtggctgttt tactgtatcg
     4441 gcctggtgta tggaacccag acattctaca acgctggaat catctacgtg gagtcttact
     4501 atatcatgcc tgccggcggc tgtaagaaac tggtgctggc catgactgcc gtgtactatt
     4561 ctagttggct gatgtttccc ggcctgttca tctttgggcc tgaaggcatg cacaccctga
     4621 gcgtggctgg gtccactatt ggccatacca tcgccgacct gctgtccaag aatatttggg
     4681 gactgctggg gcacttcctg cggatcaaaa ttcacgagca tatcattatg tacggcgata
     4741 tcaggagacc agtgagctcc cagtttctgg gacgcaaggt ggacgtgctg gccttcgtga
     4801 cagaggaaga taaagtggcg gccgccaaga gcgtcgagaa atccagaatt acttctgaag
     4861 gggagtatat ccctctggat caaatagaca tcaatgttgt cgacactagt tctagaatgg
     4921 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4981 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5041 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5101 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5161 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5221 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5281 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5341 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5401 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5461 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5521 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5581 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtctagat
     5641 tttgctatga aaatgaagtt taagaattcg atatcaagct tatcgataat caacctctgg
     5701 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5761 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5821 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5881 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5941 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6001 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6061 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     6121 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6181 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6241 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     6301 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     6361 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     6421 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     6481 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     6541 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     6601 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     6661 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     6721 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     6781 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     6841 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     6901 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     6961 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7021 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7081 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     7141 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7201 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     7261 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7321 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     7381 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     7441 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     7501 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7561 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7621 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7681 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7741 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8101 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8401 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8761 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8821 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8881 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8941 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9001 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9061 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9121 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9181 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9241 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9301 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9361 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9421 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9481 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9541 gctggccttt tgctcacatg t
//
