LOCUS       sequence-399202-        8746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-dependent version of PP_070. Co-expressing membrane-localized 
            Voltron2 and membrane-localized CheRiff..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Park P, Wong-Campos JD, Itkis DG, Lee BH, Qi Y, Davis HC, Antin B, 
            Pasarkar A, Grimm JB, Plutkis SE, Holland KL, Paninski L, Lavis LD, 
            Cohen AE
  TITLE     Dendritic excitations govern back-propagation via a spike-rate 
            accelerometer.
  JOURNAL   Nat Commun. 2025 Feb 4;16(1):1333. doi: 10.1038/s41467-025-55819-9.
  PUBMED    39905023
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-4"; volume: "16(1):1333. doi"; pages: " 
            10.1038/s41467-025-55819-9"
COMMENT     Sequence Label: 
            PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
FEATURES             Location/Qualifiers
     source          1..8746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   38..167
                     /label=AAV2 ITR
     promoter        481..758
                     /label=chicken beta-actin promoter
     primer_bind     1037..1059
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1121..1140
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1191..1224)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1275..1308)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(1347..2057,2058..2060,2061..2063))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1389..1410)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1738..1757
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1997..2018
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2163..3107)
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             complement(3126..3164)
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             complement(3225..3281)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4962..5000)
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     regulatory      5063..5072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5079..5112
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5163..5196
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5221..5809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5274..5294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5692..5703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5812..5828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5813..5829)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5858..5979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5869..5888)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5923..5942
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6141..6270
                     /label=AAV2 ITR
     rep_origin      complement(6507..7095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6587..6606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7266..8057,8058..8126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7889..7908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8127..8231)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8257..8712)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8394..8415)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8606..8625
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agggcgatcg gtgcgggcct cttcgctatt acgccagctg cgcgctcgct cgctcactga
       61 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
      121 gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt agttaatgat
      181 taacccgcca tgctacttat ctacgtagcc atgctctagg aagatctgta ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      541 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      601 ggcgcgcgcc aggsgggggg ggggggggsg aggggsgggg sggggsgagg cggagaggtg
      661 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      721 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
      781 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      841 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      901 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
      961 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg
     1021 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1081 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1141 ctgtctcatc attttggcaa agaattggat cctctagagt cgactccgga ataacttcgt
     1201 ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg ctattagcaa
     1261 tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg cgcgccttaa
     1321 acttcatttt catagcaaaa tctagacttg tacagctcgt ccatgccgag agtgatcccg
     1381 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     1441 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     1501 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     1561 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     1621 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     1681 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     1741 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     1801 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg
     1861 ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     1921 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     1981 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     2041 agctcctcgc ccttgctcac cattctagaa ctagtgtcga caacattgat gtctatttga
     2101 tccagaggga tatactcccc ttcagaagta attctggatt tctcgacgct cttggcggcc
     2161 gccactttat cttcctctgt cacgaaggcc agcacgtcca ccttgcgtcc cagaaactgg
     2221 gagctcactg gtctcctgat atcgccgtac ataatgatat gctcgtgaat tttgatccgc
     2281 aggaagtgcc ccagcagtcc ccaaatattc ttggacagca ggtcggcgat ggtatggcca
     2341 atagtggacc cagccacgct cagggtgtgc atgccttcag gcccaaagat gaacaggccg
     2401 ggaaacatca gccaactaga atagtacacg gcagtcatgg ccagcaccag tttcttacag
     2461 ccgccggcag gcatgatata gtaagactcc acgtagatga ttccagcgtt gtagaatgtc
     2521 tgggttccat acaccaggcc gatacagtaa aacagccact tcacccaccc agtggccaga
     2581 gcggctgtca ctcccatgca aatagttccc aggtcggaca ccagcagagc cattgtccgc
     2641 ttattgtatg cctcactcag gccggtgatg ttagacagat gaatcaggat cacgggacat
     2701 gtcagcagcc attcggcata tctcagccat ggggtaatgt tccctccgta caggaacagc
     2761 atagcaggac tggtgaactc gaaatagatc tccagaatca ctttgatcag ctccacggag
     2821 cacacgtaca cttcctccca tcccacgctg gccttatagg cgtgccagcc gtagaaagcc
     2881 agaaacaggg cagacaggaa cacggcaaac cactggaagc ccctagctgt ttcctgctcc
     2941 cacagggccc catagtagat gtagatggag ctgcactgtt cctcataccc atgcacgggg
     3001 tggtagggtg gattcacttg atatccagct ggggcatggt actcactgcc tccggcagaa
     3061 tcgtttccag gtggggcgct gtgagcgtct ggagcaggag ctccgccact accggatctg
     3121 actacgcctg tcttattgct gaaaggaacg tagaagttgg gaccttctgt tccgttagcc
     3181 agtccaaggg tcaacaaagc cagcaagagg agtatttggg gtcggggacc ggggttttct
     3241 tccacgtctc ctgcttgctt taacagagag aagttcgtgg ctccggagcc cacctcgttc
     3301 tcgtagcaga acacgttgat gtcgatctgg tccaggggga tgtactcgcc ctcgctggtg
     3361 atcctgctct tagtggttgg ctcgccggaa atctcgagcg tcgacagcca gcgcgcgatc
     3421 tcgctgccga tcaggtccgg gttgtcttct tgcagcagat tcagacccgg gccgatgtcc
     3481 acagccttgc agttaggcag gcttttggcc aggcgagcgg cttcggccgg tgggatcaga
     3541 acgcctgggg tgccccagaa cagcagcttc gggacagggg actggtgcag ccagtccatg
     3601 tattcttcga ccagcgcgac gatgttcgct ggctcaccgg cgattggcag ctcgtttggg
     3661 aagcgccaca gtggctcgcg gtcaacagga ttcaggaacg gctcgcggta atggtccatc
     3721 tcgacttcag tcagcgggcg gacgacaccc atcggcagcg taccctcgat aaaaacgttc
     3781 tgatcgatga tcagcttgcg gccgacgtcg gtggtgcgga aggcctggaa ggtctcgcgg
     3841 gcaaattctg gccattcgtc ccaggtcggg atagggcgga tgaactccat aaatgcaata
     3901 cctttgacgc gctctggatt gcgcttggcc cagtggaaac ccagagcgga gccccagtcg
     3961 tgaatgacca ggacgacctc ttccagaccc agggcttcga tgaaggcatc catgaagcgg
     4021 acgtggtcgt cgaagaaata acccaggtct ggtttgtcgg atttgcccat accgatcagg
     4081 tctggagcaa tgcagcgatg ggtcggtgca acatgcggga tgatgttgcg ccacacgtag
     4141 gaggaggtcg ggttaccgtg caggaacagc acaggggtgc catcgcgcgg accaacatcg
     4201 acgtagtgca tgcgctcgcc caggacttcc acataatggg ggtcgaatgg aaagccagta
     4261 ccgatcttga agatagtctc atgggcaatg aggcacccca ttccaaacag tggcttagcg
     4321 agcacatcca ggattcccat gagaatggcc tcattgtcga cggacagcac gccactacca
     4381 gacccgaaag cccacacgac aggataaaac acccagagga ttgacaggaa gacgagcaga
     4441 ctgacatagg agctatggta ctcggggctt ttcttcagat cgtctttcca agtggcgtta
     4501 tacatcccgt atgtcaggac tgcctgcatc acacatccag cgataaagta cacccatttg
     4561 aacttgtgtt catcctcgaa gtcgcccaga ataccaaaca cgatcatgaa aatgtctgcg
     4621 ccgatgaccc aagaaatcat cactccgccc atcttggtca tgacgatgag atcgagcagg
     4681 agcagtggtg tggtcagcac ccagttaata tatcttgcgt agaccacctg cctttcacca
     4741 ttcattgtca ggtcctggta gttcacggcc attgcatagt aagcggtagc ggcgatagtg
     4801 cagatggcaa tgttgatata gtatgcgctc ttcagctctg aaggccgaaa gtgcacagcc
     4861 actccaaaag caatagcagc agcagccata atagcaaaga caatccactg tgcaatcatg
     4921 ccggtctcgg tttccacgtc agcactaccg gatctgacta cgcctgtctt attgctgaaa
     4981 ggaacgtaga agttgggacc ttctgttccg ttagccagtc caagggtcaa caaagccagc
     5041 aagaggagta tttggggtcg gcccatggtg gcgctagcat aacttcgtat aaagtatcct
     5101 atacgaagtt atttgcctta acccagaaat tatcactgtt attctttaga atggtgcaaa
     5161 gaataacttc gtataatgta tgctatacga agttatgaat tcgatatcaa gcttatcgat
     5221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     5821 cgacctcgac ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     5881 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     5941 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6001 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     6061 aaatgtggta aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg
     6121 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     6181 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     6241 ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg
     6301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8221 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     8281 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8341 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8401 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8461 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8521 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8581 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8641 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     8701 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttggga
//
