LOCUS       sequence-399212-        8159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DOX-inducible expression of a short-hairpin RNA targetinging human 
            BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pRSITEP-shBIRC6-2
FEATURES             Location/Qualifiers
     source          1..8159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..107
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     301..323
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     315..332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     316..332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        347..573
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             574..754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    801..926
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1419..1652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1837..1881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2160..2180
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2331..2350
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    2387..2405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2560..2677
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2756..2967
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2980..3248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3280..3900
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             3907..3960
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3967..4566
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3967..3986)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4463..4483
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4579..5167
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4632..4652)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5050..5061)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5241..5474
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5546..5667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5583..5602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5637..5656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5707..5842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5875..5891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5875..5891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5888..5910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5899..5915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5923..5929,5930..5947,5948..5953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5968..5989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6106..6123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6277..6865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7036..7827,7828..7896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7659..7678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7897..8001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8069..8087
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8125..8147)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
       61 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      121 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
      181 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      241 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      301 cccagtcacg acgttgtaaa acgacggcca gtgccaagct gacgcgtgta gtcttatgca
      361 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      421 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      481 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      541 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      721 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      781 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      841 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      901 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      961 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1021 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1081 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1141 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1201 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1261 aagcggccac tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1321 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1381 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1441 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1501 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1561 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1621 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1681 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1741 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1801 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1861 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1921 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1981 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2041 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2101 gacagatcca ttcgattagt gaacggatct cgacggtatc gatggccgcc cccttcaccg
     2161 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     2221 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     2281 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     2341 tgcttaccgt aacttgaaag ccctatcagt gatagagatt tatatatccc tatcagtgat
     2401 agagacaccg gccaggagca acacaacatt ccgttaatat tcatagcgga atgttgtgtt
     2461 gctcctggtt ttttcggact gtagaactct gaacctctcg gtggtnnccg tatcattaga
     2521 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2581 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2641 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2701 agatccactt tggccgccga ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca
     2761 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     2821 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     2881 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     2941 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     3001 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     3061 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     3121 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     3181 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     3241 tacagatcca agctgtgacc ggcgcctact ctagacacca tgtccagact ggacaagagc
     3301 aaagtcatca actctgcctt ggagctcctg aatgaagttg gcattgaggg cttgaccacc
     3361 aggaagctgg cccagaagct gggtgtggag cagcctaccc tgtactggca tgtgaagaac
     3421 aagagggctc tgcttgatgc cctggccatt gagatgttgg acaggcacca cacccacttc
     3481 tgccctctgg aaggggagtc ctggcaggac ttcctgagga acaatgccaa gagcttcaga
     3541 tgtgccttgc tctcccaccg ggacggtgcc aaagttcact tgggcaccag gcctacagag
     3601 aagcagtatg agaccctgga gaaccagctg gcattcctgt gccaacaagg cttctccctg
     3661 gaaaatgcct tgtatgccct ctctgctgtg ggccacttca ccttgggctg tgtgctggag
     3721 gaccaggagc accaagttgc caaggaggag agggagaccc ccaccactga ctccatgcca
     3781 ccactgctgc ggcaagctat tgagttgttt gaccaccaag gggctgagcc tgcattcctg
     3841 tttggcctgg aactgatcat ctgtggcctg gaaaagcagc tgaaatgtga gtctggctct
     3901 ggatccgagg gcagaggaag ccttctaaca tgcggtgacg tggaggagaa tcccggccct
     3961 tccgggatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4021 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4081 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     4141 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     4201 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     4261 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     4321 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     4381 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     4441 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     4501 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     4561 gcctgaatct aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4621 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4681 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4741 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4801 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4861 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4921 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4981 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5041 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5101 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctctct ttgggccgcc
     5161 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5221 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     5281 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5461 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5521 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     5581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5641 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     5701 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5761 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5821 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     5881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6061 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8041 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     8101 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacag
//
