LOCUS       sequence-399223-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a guide RNA targeting BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pXPR_003-sgBIRC6-1
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..41
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    158..179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(194..199,200..217,218..224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     237..259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     256..272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     256..272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     291..311
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        293..311
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        339..565
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             566..746
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    793..918
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1411..1644
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1829..1873
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2051..2299
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2051..2071
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2222..2241
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2321..2396
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2452..2569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2708..3886
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2939..3877
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3834..3854
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3899..4498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3899..3918)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4395..4415
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4514..5102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4567..4587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4985..4996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5174..5407
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5485..5606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5522..5541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5576..5595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5646..5781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5801..5820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5802..5820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5830..5847)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5830..5846)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5839..5861)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5988..6443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6075..6094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6285..6306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6469..6573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6574..6642,6643..7434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6792..6811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7605..8193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8094..8113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
       61 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      121 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
      301 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
      361 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
      421 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
      481 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
      541 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
      601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      721 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      781 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      841 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      901 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
      961 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1021 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1081 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1141 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1201 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1261 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1321 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1381 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1441 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1501 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1561 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1621 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1681 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1741 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1801 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1861 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1921 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1981 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2041 gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     2101 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     2161 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     2221 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     2281 gtggaaagga cgaaacaccg ttgcactcaa ggattcaaca gttttagagc tagaaatagc
     2341 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2401 ttaagcttgg cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     2461 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2521 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2581 cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat
     2641 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     2701 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2761 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     2821 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2881 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     2941 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     3001 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     3061 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     3121 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     3181 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     3241 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     3301 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     3361 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     3421 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     3481 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     3541 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     3601 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     3661 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     3721 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     3781 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     3841 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta cggccaccat
     3901 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3961 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4021 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4081 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     4141 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4201 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4261 cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag
     4321 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4381 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4441 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac
     4501 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4561 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4621 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4681 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4741 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4801 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4861 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4921 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4981 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5041 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5101 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5161 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5401 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5461 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5581 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     5641 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5701 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5761 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     5821 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5881 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5941 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6421 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7021 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7081 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7141 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7201 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7261 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7321 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7381 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7441 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7501 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7561 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7621 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7681 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7741 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7801 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7861 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7921 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7981 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8041 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8101 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8161 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8221 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8281 gtggataacc gtattaccgc ctttgagtga gctgataccg ctc
//
