LOCUS       sequence-399227-        9778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and 
            Tet-On 3G transactivator (Tet3G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pLX209-neo-HA-Ub-T2A-Tet3G
FEATURES             Location/Qualifiers
     source          1..9778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(139..144,145..162,163..169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    177..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     182..204
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     201..217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     201..217
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     236..256
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        238..256
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        284..510
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             511..691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    738..863
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1356..1589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1774..1818
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1993..2493
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2412..2430
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2508..3302
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDRLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2562..2581)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3172..3191
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3350..3467
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3606..4784
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3837..4775
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4732..4752
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4792..4801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4804..4830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4861..5088
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             4861..5088
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             5098..5151
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5152..5898
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5914..6502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5967..5987)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6385..6396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6574..6807
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6885..7006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6922..6941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6976..6995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7046..7181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7201..7220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7202..7220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7230..7247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7230..7246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7239..7261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7388..7843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7475..7494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7685..7706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7869..7973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7974..8042,8043..8834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8192..8211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9005..9593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9494..9513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9747..9764
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
       61 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
      241 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
      301 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
      361 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
      421 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
      481 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
      541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      661 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      721 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      781 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      841 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      901 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      961 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1021 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1081 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1141 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1201 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1261 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1321 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1381 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1441 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1501 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1561 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1621 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1681 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1741 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1801 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1861 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1921 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     1981 ccgaggggac ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     2041 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     2101 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     2161 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     2221 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     2281 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     2341 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     2401 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     2461 ctcgttgacc gaatcaccga cctctctccc cagggggtac cgccaccatg attgaacaag
     2521 atagattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     2581 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     2641 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     2701 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     2761 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     2821 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     2881 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     2941 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3001 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3061 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3121 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3181 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     3241 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     3301 gaggtggcga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     3361 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3421 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     3481 gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa
     3541 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     3601 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     3661 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     3721 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3781 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3841 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3901 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     3961 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     4021 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     4081 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     4141 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     4201 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     4261 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     4321 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     4381 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     4441 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     4501 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     4561 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     4621 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     4681 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     4741 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagggatc cgccaccatg
     4801 ggctacccct atgatgtgcc tgactacgca gatctcaatg gtggtggtgg tgggtcgacc
     4861 atgcagatct tcgtgaagac cctgactggt aagaccatca ctctcgaagt ggagccgagt
     4921 gacaccattg agaatgtcaa ggcaaagatc caagacaagg aaggcatccc tcctgaccag
     4981 cagaggttga tctttgctgg gaaacagctg gaagatggac gcaccctgtc tgactacaac
     5041 atccagaaag agtccaccct gcacctggtc ctccgtctca gaggtggtgg atccggtgag
     5101 ggcagaggaa gccttctaac atgcggtgac gtggaggaga atcccggccc tatgtctaga
     5161 ctggacaaga gcaaagtcat aaactctgct ctggaattac tcaatggagt cggtatcgaa
     5221 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     5281 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     5341 catacccact cctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     5401 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     5461 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     5521 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     5581 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     5641 gattctatgc ccccacttct gaaacaagca attgagctgt tcgaccggca gggagccgaa
     5701 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     5761 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     5821 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     5881 gacatgctcc ccgggtaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     5941 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6001 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6061 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6121 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6181 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6241 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6301 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6361 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6421 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6481 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6541 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6601 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6781 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     6841 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6901 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6961 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     7021 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     7201 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7261 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7321 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7381 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7681 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7801 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7861 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7981 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8041 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8101 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8161 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8221 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8281 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8341 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8401 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8461 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8521 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8581 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8641 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8701 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8761 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8821 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8881 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9061 taccagcggt ggtttgtttg ccggatcaag agccaccaac tctttttccg aaggtaactg
     9121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcg
//
