LOCUS       sequence-39923-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Foley JE, Maeder ML, Pearlberg J, Joung JK, Peterson RT, Yeh JR
  TITLE     Targeted mutagenesis in zebrafish using customized zinc-finger 
            nucleases.
  JOURNAL   Nat Protoc. 2009. 4(12):1855-67.
  PUBMED    20010934
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2009. 4(12):1855-67."
COMMENT     Sequence Label: pMLM292
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcaccat gggacctaag aaaaagagga aggtggcggc cgctgactac aaggatgacg
      121 acgataaatc tagatagtaa gcttggatcc caactagtca aaagtgaact ggaggagaag
      181 aaatctgaac ttcgtcataa attgaaatat gtgcctcatg aatatattga attaattgaa
      241 attgccagaa attccactca ggatagaatt cttgaaatga aggtaatgga attttttatg
      301 aaagtttatg gatatagagg taaacatttg ggtggatcaa ggaaaccgga cggagcaatt
      361 tatactgtcg gatctcctat tgattacggt gtgatcgtgg atactaaagc ttatagcgga
      421 ggttataatc tgccaattgg ccaagcagat gaaatggagc gatatgtcga agaaaatcaa
      481 acacgaaaca aacatctcaa ccctaatgaa tggtggaaag tctatccatc ttctgtaacg
      541 gaatttaagt ttttatttgt gagtggtcac tttaaaggaa actacaaagc tcagcttaca
      601 cgattaaatc atatcactaa ttgtaatgga gctgttctta gtgtagaaga gcttttaatt
      661 ggtggagaaa tgattaa
//
