LOCUS       sequence-39924-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Shin DH, Oganesyan N, Jancarik J, Yokota H, Kim R, Kim SH
  TITLE     Crystal structure of a nicotinate phosphoribosyltransferase from 
            Thermoplasma acidophilum.
  JOURNAL   J Biol Chem. 2005 May 6. 280(18):18326-35.
  PUBMED    15753098
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 May 6. 280(18):18326-35."
COMMENT     Sequence Label: pLIC.B4.Ta1145
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(176..196)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(227..250)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      358..367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatttatcca tgtatccaat ggcccggaaa cggtggcctc catggcgacc ctcagatcat
       61 tgcatttatc gcctatagcc gatattgtcc tctcaaaata gacatccgaa gccagcccct
      121 tttttatatc ttcatcactt gctgtgttga acacattcat gccgccacca ccgccgccct
      181 ggaagtaaag gttttcggtc gttgggatat cgtaatcggt accagtctgc gcgtctttca
      241 gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat
      301 accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca
      361 tggtggcggc aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg
      421 cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc agatagtttt
      481 cgaggaactc ttttgccagc tctttgttcg gactggcggc gttaatacct gcgctcagca
      541 cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat
      601 tcactttgct ggtgtcgagg ttgga
//
