LOCUS       sequence-399291-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0172
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             553..576
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tgtcgtgagg ctagcaccgc caccatgaag gctgcctaca ccgcctatcg atgcctcacc
       61 aaagacctag aaggctgcgc catgaacccg gagctgacaa tggaaagtct gggcactttg
      121 cacgggccgg ccggcggcgg cagtggcggg ggcggcggcg ggggcggcgg gggcggcggc
      181 gggggcccgg gccatgagca ggagctgctg gccagcccca gcccccacca cgcgggccgc
      241 ggcgccgctg gctcgctgcg gggccctccg ccgcctccaa ccgcgcacca ggagctgggc
      301 acggcggcag cggcggcagc ggcggcgtcg cgctcggcca tggtcaccag catggcctcg
      361 atcctggacg gcggcgacta ccggcccgag ctctccatcc cgctgcacca cgccatgagc
      421 atgtcctgcg actcgtctcc gcctggcatg ggcatgagca acacctacac cacgctgaca
      481 ccgctccagc cgctgccacc catctccacc gtgtctgaca agttccacca ccctcacccg
      541 caccaccatc cgcaccacca ccaccaccac caccaccagc gcctgtccgg caacgtcagc
      601 ggcagcttca ccctcatgcg cgacgagcgc gggctcccgg ccatgaacaa cctctacagt
      661 ccctacaagg agatgcccgg catgagccag agcctgtccc cgctggccgc cacgccgctg
      721 ggcaacgggc taggcggcct ccacaacgcg cagcagagtc tgcccaacta cggtccgccg
      781 ggccacgaca aaatgctcag ccccaacttc gacgcgcacc acactgccat gctgacccgc
      841 ggtgagcaac acctgtcccg cggcctgggc accccacctg cggccatgat gtcgcacctg
      901 aacggcctgc accacccggg ccacactcag tctcacgggc cggtgctggc acccagt
//
