LOCUS       sequence-399300-       10490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HRI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10490)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 10490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pLX_314-HRI-V5
FEATURES             Location/Qualifiers
     source          1..10490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    100..121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(136..141,142..159,160..166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    174..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     179..201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     198..214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     198..214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     233..253
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        235..253
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        281..507
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             508..688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    735..860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1353..1586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1771..1815
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2046..2256
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2068..2264
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2115..2250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2289..3083
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDRLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2343..2362)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2953..2972
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3131..3248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3386..4564
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3617..4555
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4512..4532
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4584..4608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6505..6529)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6531..6572
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6623..7211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6676..6696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7094..7105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7283..7516
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7594..7715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7631..7650)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7685..7704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7755..7890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7910..7929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7911..7929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7939..7956)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7939..7955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7948..7970)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8097..8552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8184..8203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8394..8415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8578..8682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8683..8751,8752..9543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8901..8920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9714..10302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10203..10222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10456..10473
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
       61 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      121 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      181 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      241 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      301 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      361 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      421 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      481 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
      541 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      601 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      661 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      721 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      781 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      841 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      901 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      961 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1021 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1081 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1141 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1201 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1261 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1321 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1381 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1441 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1501 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1561 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1621 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1681 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1741 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1801 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1861 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1921 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     1981 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2041 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
     2101 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2161 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2221 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
     2281 ccgccaccat gattgaacaa gatagattgc acgcaggttc tccggccgct tgggtggaga
     2341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     2461 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     2521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     2581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     2641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     2701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     2761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     2821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     2881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     2941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3061 gccttcttga cgagttcttc tgaggtggcg aattctagat cttgagacaa atggcagtat
     3121 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3181 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3241 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     3301 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     3361 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     3421 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     3481 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     3541 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3601 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     3661 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     3721 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     3781 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     3841 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     3901 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     3961 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     4021 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     4081 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     4141 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     4201 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     4261 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     4321 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     4381 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     4441 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     4501 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     4561 gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggcttc accatgcagg
     4621 ggggcaactc cggggtccgc aagcgcgaag aggagggcga cggggctggg gctgtggctg
     4681 cgccgccggc catcgacttt cccgccgagg gcccggaccc cgaatatgac gaatctgatg
     4741 ttccagcaga aatccaggtg ttaaaagaac ccctacaaca gccaaccttc ccttttgcag
     4801 ttgcaaacca actcttgctg gtttctttgc tggagcactt gagccacgtg catgaaccaa
     4861 acccacttcg ttcaagacag gtgtttaagc tactttgcca gacgtttatc aaaatggggc
     4921 tgctgtcttc tttcacttgt agtgacgagt ttagctcatt gagactacat cacaacagag
     4981 ctattactca cttaatgagg tctgctaaag agagagttcg tcaggatcct tgtgaggata
     5041 tttctcgtat ccagaaaatc agatcaaggg aagtagcctt ggaagcacaa acttcacgtt
     5101 acttaaatga atttgaagaa cttgccatct taggaaaagg tggatacgga agagtataca
     5161 aggtcaggaa taaattagat ggtcagtatt atgcaataaa aaaaatcctg attaagggtg
     5221 caactaaaac agtttgcatg aaggtcctac gggaagtgaa ggtgctggca ggtcttcagc
     5281 accccaatat tgtcggttac catactgcat ggatagaaca tgttcatgtg attcagccac
     5341 gagcagacag agctgccatt gagttgccat ctctggaagt gctctccgac caggaagagg
     5401 acagagagca atgtggtgtt aaaaatgatg aaagtagcag ctcatccatt atctttgctg
     5461 agcccacccc agaaaaagaa aaacgctttg gagaatctga cactgaaaat cagaataaca
     5521 agtcggtgaa gtacaccacc aatttagtca taagagaatc tggtgaactt gagtcgaccc
     5581 tggagctcca ggaaaatggc ttggctggtt tgtctgccag ttcaattgtg gaacagcagc
     5641 tgccactcag gcgtaattcc cacctagagg agagtttcac atccaccgaa gaatcttccg
     5701 aagaaaatgt caactttttg ggtcagacag aggcacagta ccacctgatg ctgcacatcc
     5761 agatgcagct gtgtgagctc tcgctgtggg attggatagt cgagagaaac aagcggggcc
     5821 gggagtatgt ggacgagtct gcctgtcctt atgttatggc caatgttgca acaaaaattt
     5881 ttcaagaatt ggtagaaggt gtgttttaca tacataacat gggaattgtg caccgagatc
     5941 tgaagccaag aaatattttt cttcatggcc ctgatcagca agtaaaaata ggagactttg
     6001 gtctggcctg cacagacatc ctacagaaga acacagactg gaccaacaga aacgggaaga
     6061 gaacaccaac acatacgtcc agagtgggta cttgtctgta cgcttcaccc gaacagttgg
     6121 aaggatctga gtatgatgcc aagtcagata tgtacagctt gggtgtggtc ctgctagagc
     6181 tctttcagcc gtttggaaca gaaatggagc gagcagaagt tctaacaggt ttaagaactg
     6241 gtcagttgcc ggaatccctc cgtaaaaggt gtccagtgca agccaagtat atccagcact
     6301 taacgagaag gaactcatcg cagagaccat ctgccattca gctgctgcag agtgaacttt
     6361 tccaaaattc tggaaatgtt aacctcaccc tacagatgaa gataatagag caagaaaaag
     6421 aaattgcaga actaaagaag cagctaaacc tcctttctca agacaaaggg gtgagggatg
     6481 acggaaagga tgggggcgtg ggatgcccaa ctttcttgta caaagtggtt ggtaagccta
     6541 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagtacc ggttaagtcg
     6601 acaatcaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6661 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6721 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6781 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6841 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6901 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6961 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7021 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7081 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7141 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7201 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7261 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7321 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7381 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7441 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7501 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     7561 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7621 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7681 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7741 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7861 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7921 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7981 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8041 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8521 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8761 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8821 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8881 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8941 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9001 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9061 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9121 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9181 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9241 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9301 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9361 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9421 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9481 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9541 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9601 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9661 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9721 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9781 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9841 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9901 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9961 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10021 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10081 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10141 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10201 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10261 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10321 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10381 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10441 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
//
