LOCUS       sequence-399301-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a guide RNA targeting UBR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pXPR_003-sgUBR4
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..62
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    179..200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(215..220,221..238,239..245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    253..269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     258..280
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     277..293
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     277..293
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     312..332
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        314..332
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        360..586
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             587..767
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    814..939
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1432..1665
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1850..1894
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2072..2320
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2072..2092
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2243..2262
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2342..2417
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2473..2590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2729..3907
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2960..3898
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3855..3875
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3920..4519
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3920..3939)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4416..4436
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4535..5123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4588..4608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5006..5017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5195..5428
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5506..5627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5543..5562)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5597..5616
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5667..5802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5822..5841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5823..5841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5851..5868)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5851..5867)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5860..5882)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6009..6464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6096..6115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6306..6327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6490..6594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6595..6663,6664..7455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6813..6832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7626..8214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8115..8134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
       61 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      121 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      181 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      241 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      301 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
      361 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
      421 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
      481 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
      541 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
      601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      781 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      841 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      901 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      961 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1021 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1081 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1141 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1201 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1261 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1321 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1381 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1441 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1501 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1561 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1621 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1681 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1741 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1801 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1861 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     1921 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     1981 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2041 gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga ttccttcata
     2101 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
     2161 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     2221 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     2281 tttcttggct ttatatatct tgtggaaagg acgaaacacc gggaaccgat tgatatagcg
     2341 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     2401 tggcaccgag tcggtgcttt tttaagcttg gcgtaactag atcttgagac aaatggcagt
     2461 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2521 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2581 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     2641 ggcccgggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     2701 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2761 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2821 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2881 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2941 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3001 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     3061 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     3121 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     3181 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     3241 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     3301 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3361 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3421 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3481 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3541 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3601 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3661 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3721 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     3781 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     3841 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     3901 gtcgtgacgt acggccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3961 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4021 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4081 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     4141 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4201 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4261 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca
     4321 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4381 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4441 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4501 ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     4561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5101 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     5161 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5221 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5281 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5341 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5401 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     5461 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     5521 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5581 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     5641 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5701 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5761 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     5821 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     5881 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5941 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6001 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6061 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6301 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6361 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6421 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8281 cctgcgttat cccctgattc tgtggataac cgtattaccg cct
//
