LOCUS       sequence-399303-        8159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a seed-matched control for pRSITEP-puro-shBIRC6-2(3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pRSITEP-shBIRC6-2(3)-C911
FEATURES             Location/Qualifiers
     source          1..8159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..124
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(162..184)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     284..303
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     497..519
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     511..528
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     512..528
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        543..769
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             770..950
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    997..1122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1615..1848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2033..2077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2356..2376
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2527..2546
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    2583..2601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2756..2873
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2952..3163
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3176..3444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3476..4096
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             4103..4156
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4163..4762
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4163..4182)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4659..4679
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4775..5363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4828..4848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5246..5257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5437..5670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5742..5863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5779..5798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5833..5852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5903..6038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6071..6087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6071..6087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6084..6106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6095..6111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6119..6125,6126..6143,6144..6149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6164..6185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6302..6319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6473..7061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7232..8023,8024..8092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7855..7874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8093..8159,1..38))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
       61 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      121 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
      181 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
      241 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
      301 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
      361 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      421 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      481 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgacg
      541 cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      601 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      661 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      721 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      961 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1021 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1081 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1141 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1201 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1261 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1321 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1381 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1441 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga
     1501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1561 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1621 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1681 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1801 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1921 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1981 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2101 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2161 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2221 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2281 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatg
     2341 gccgccccct tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac
     2401 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     2461 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     2521 aaaatggact atcatatgct taccgtaact tgaaagccct atcagtgata gagatttata
     2581 tatccctatc agtgatagag acaccgggcc tcgtttgaac ggaacctagt taatattcat
     2641 agctaggttc cgttcaaacg aggctttttt cggactgtag aactctgaac ctctcggtgg
     2701 tcgccgtatc attagaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
     2761 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2821 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2881 attacaggga cagcagagat ccactttggc cgccgactag tggatctgcg atcgctccgg
     2941 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     3001 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     3061 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     3121 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     3181 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     3241 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     3301 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     3361 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     3421 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctactctag acaccatgtc
     3481 cagactggac aagagcaaag tcatcaactc tgccttggag ctcctgaatg aagttggcat
     3541 tgagggcttg accaccagga agctggccca gaagctgggt gtggagcagc ctaccctgta
     3601 ctggcatgtg aagaacaaga gggctctgct tgatgccctg gccattgaga tgttggacag
     3661 gcaccacacc cacttctgcc ctctggaagg ggagtcctgg caggacttcc tgaggaacaa
     3721 tgccaagagc ttcagatgtg ccttgctctc ccaccgggac ggtgccaaag ttcacttggg
     3781 caccaggcct acagagaagc agtatgagac cctggagaac cagctggcat tcctgtgcca
     3841 acaaggcttc tccctggaaa atgccttgta tgccctctct gctgtgggcc acttcacctt
     3901 gggctgtgtg ctggaggacc aggagcacca agttgccaag gaggagaggg agacccccac
     3961 cactgactcc atgccaccac tgctgcggca agctattgag ttgtttgacc accaaggggc
     4021 tgagcctgca ttcctgtttg gcctggaact gatcatctgt ggcctggaaa agcagctgaa
     4081 atgtgagtct ggctctggat ccgagggcag aggaagcctt ctaacatgcg gtgacgtgga
     4141 ggagaatccc ggcccttccg ggatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4201 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4261 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4321 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4381 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4441 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4501 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4561 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4621 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4681 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4741 gacccgcaag cccggtgcct gaatctaggt cgacaatcaa cctctggatt acaaaatttg
     4801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5161 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5281 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     5341 ctctctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     5401 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5461 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5641 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5701 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     5761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     5881 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6001 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat
     6061 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7081 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7141 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7201 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7261 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7321 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7381 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7441 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7501 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7561 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7621 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7681 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7741 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7801 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7861 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7921 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7981 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8041 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8101 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatattt
//
