LOCUS       sequence-399305-       12045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expression of HRI and TagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pTRE-HRI-V5-IRES-BFP
FEATURES             Location/Qualifiers
     source          1..12045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    203..337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(240..259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     294..313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             505..1138
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1185..1310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1807..2040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2225..2269
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2554..2671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2733..3082
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2741..2759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2777..2795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2813..2831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2849..2867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2885..2903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2921..2939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2957..2975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3021..3045
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(4996..5020)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5022..5063
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(5081..5655,5656..5658,5659..5667)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5248..5265)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5475..5494
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5668..6369
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6413..7001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6466..6486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6884..6895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7004..7020)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7005..7021)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7111..7344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7369..7387)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7370..7387)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7677..7696)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7910..7929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8029..8051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8089..8107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8175..8279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8280..8348,8349..9140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8498..8517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9311..9899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9800..9819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10053..10070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10140..10160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10145..10474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10325..10460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11608..11673
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11803..11823
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
       61 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      121 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      181 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      301 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      361 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      421 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      481 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
      541 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
      601 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
      661 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
      721 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
      781 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
      841 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
      901 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
      961 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1021 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1081 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1141 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1201 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1261 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1321 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1381 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1441 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1501 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1561 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1621 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1681 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1741 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1801 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1861 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1921 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1981 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     2041 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     2101 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     2161 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     2221 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     2281 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     2341 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2401 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2461 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     2521 acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2581 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2641 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2701 agtttatcga tgaggccctt tcgtcttcac tcgagtttac tccctatcag tgatagagaa
     2761 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     2821 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     2881 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     2941 cgtatatcca gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg
     3001 gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc
     3061 acaacacttt tgtcttatac ttacgcgtaa aagcaggctt caccatgcag gggggcaact
     3121 ccggggtccg caagcgcgaa gaggagggcg acggggctgg ggctgtggct gcgccgccgg
     3181 ccatcgactt tcccgccgag ggcccggacc ccgaatatga cgaatctgat gttccagcag
     3241 aaatccaggt gttaaaagaa cccctacaac agccaacctt cccttttgca gttgcaaacc
     3301 aactcttgct ggtttctttg ctggagcact tgagccacgt gcatgaacca aacccacttc
     3361 gttcaagaca ggtgtttaag ctactttgcc agacgtttat caaaatgggg ctgctgtctt
     3421 ctttcacttg tagtgacgag tttagctcat tgagactaca tcacaacaga gctattactc
     3481 acttaatgag gtctgctaaa gagagagttc gtcaggatcc ttgtgaggat atttctcgta
     3541 tccagaaaat cagatcaagg gaagtagcct tggaagcaca aacttcacgt tacttaaatg
     3601 aatttgaaga acttgccatc ttaggaaaag gtggatacgg aagagtatac aaggtcagga
     3661 ataaattaga tggtcagtat tatgcaataa aaaaaatcct gattaagggt gcaactaaaa
     3721 cagtttgcat gaaggtccta cgggaagtga aggtgctggc aggtcttcag caccccaata
     3781 ttgtcggtta ccatactgca tggatagaac atgttcatgt gattcagcca cgagcagaca
     3841 gagctgccat tgagttgcca tctctggaag tgctctccga ccaggaagag gacagagagc
     3901 aatgtggtgt taaaaatgat gaaagtagca gctcatccat tatctttgct gagcccaccc
     3961 cagaaaaaga aaaacgcttt ggagaatctg acactgaaaa tcagaataac aagtcggtga
     4021 agtacaccac caatttagtc ataagagaat ctggtgaact tgagtcgacc ctggagctcc
     4081 aggaaaatgg cttggctggt ttgtctgcca gttcaattgt ggaacagcag ctgccactca
     4141 ggcgtaattc ccacctagag gagagtttca catccaccga agaatcttcc gaagaaaatg
     4201 tcaacttttt gggtcagaca gaggcacagt accacctgat gctgcacatc cagatgcagc
     4261 tgtgtgagct ctcgctgtgg gattggatag tcgagagaaa caagcggggc cgggagtatg
     4321 tggacgagtc tgcctgtcct tatgttatgg ccaatgttgc aacaaaaatt tttcaagaat
     4381 tggtagaagg tgtgttttac atacataaca tgggaattgt gcaccgagat ctgaagccaa
     4441 gaaatatttt tcttcatggc cctgatcagc aagtaaaaat aggagacttt ggtctggcct
     4501 gcacagacat cctacagaag aacacagact ggaccaacag aaacgggaag agaacaccaa
     4561 cacatacgtc cagagtgggt acttgtctgt acgcttcacc cgaacagttg gaaggatctg
     4621 agtatgatgc caagtcagat atgtacagct tgggtgtggt cctgctagag ctctttcagc
     4681 cgtttggaac agaaatggag cgagcagaag ttctaacagg tttaagaact ggtcagttgc
     4741 cggaatccct ccgtaaaagg tgtccagtgc aagccaagta tatccagcac ttaacgagaa
     4801 ggaactcatc gcagagacca tctgccattc agctgctgca gagtgaactt ttccaaaatt
     4861 ctggaaatgt taacctcacc ctacagatga agataataga gcaagaaaaa gaaattgcag
     4921 aactaaagaa gcagctaaac ctcctttctc aagacaaagg ggtgagggat gacggaaagg
     4981 atgggggcgt gggatgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
     5041 ctctcctcgg tctcgattct acgtagaatt ccgccccccc cccctctccc tccccccccc
     5101 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5161 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5221 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5281 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5341 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5401 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5461 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5521 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5581 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5641 aaacacgatg ataatatggc cacaaccatg agcgagctga ttaaggagaa catgcacatg
     5701 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     5761 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     5821 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     5881 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     5941 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     6001 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     6061 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     6121 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     6181 gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     6241 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     6301 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     6361 cttaattaaa ctagtcctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     6421 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6481 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6541 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6601 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6661 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6721 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6781 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6841 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6901 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6961 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     7021 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7081 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7141 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7321 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     7381 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     7441 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     7501 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     7561 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     7621 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     7681 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     7741 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     7801 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     7861 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     7921 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7981 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8041 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     8101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9961 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10021 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10081 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
    10141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10381 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10441 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    10501 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    10561 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    10621 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    10681 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    10741 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    10801 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    10861 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    10921 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    10981 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    11041 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    11101 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    11161 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    11221 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    11281 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    11341 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    11401 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    11461 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    11521 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    11581 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    11641 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    11701 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    11761 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    11821 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    11881 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    11941 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    12001 cataacatac tgttttttct tactccacac aggcatagag tgtct
//
