LOCUS       sequence-399316-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LRP1 protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LRP1A-c004
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             115..135
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             616..660
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atggtaagcg ctattgtttt atatgtgctt ttggcggcgg cggcgcattc tgcctttgcg
       61 gccgccatgg gccaccatca tcatcatcat tcttctggtg tagatctggg taccgagaac
      121 ctgtacttcc aatccatggc tatcgacgcc cctaagactt gcagccccaa gcagtttgcc
      181 tgcagagatc aaataacctg tatctcaaag ggctggcggt gcgacggtga gagggactgc
      241 ccagacggat ctgacgaggc ccctgagatt tgtccacaga gtaaggccca gcgatgccag
      301 ccaaacgagc ataactgcct gggtactgag ctgtgtgttc ccatgtcccg cctctgcaat
      361 ggggtccagg actgcatgga cggctcagat gaggggcccc actgccgaga gctccaaggc
      421 aactgctctc gcctgggctg ccagcaccat tgtgtcccca cactcgatgg gcccacctgc
      481 tactgcaaca gcagctttca gcttcaggca gatggcaaga cctgcaaaga ttttgatgag
      541 tgctcagtgt acggcacctg cagccagcta tgcaccaaca cagacggctc cttcataagc
      601 agtaaaggtg gatacggcct gaatgatatc tttgaagcgc agaagattga atggcatgaa
      661 tga
//
