LOCUS       sequence-399330-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TMEFF2 protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TMEFF2A-c004
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             100..108
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             904..948
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             961..978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggatcc ttcccagccc tgggatgcct gcgctgctct ccctcgtgag ccttctctcc
       61 gtgctgctga tgggttgcgt agctgaaacc ggtttcccta cctccttaag tgactgccaa
      121 acgcccaccg gctggaattg ctctggttat gatgacagag aaaatgatct cttcctctgt
      181 gacaccaaca cctgtaaatt tgatggggaa tgtttaagaa ttggagacac tgtgacttgc
      241 gtctgtcagt tcaagtgcaa caatgactat gtgcctgtgt gtggctccaa tggggagagc
      301 taccagaatg agtgttacct gcgacaggct gcatgcaaac agcagagtga gatacttgtg
      361 gtgtcagaag gatcatgtgc cacagatgca ggatcaggat ctggagatgg agtccatgaa
      421 ggctctggag aaactagtca aaaggagaca tccacctgtg atatttgcca gtttggtgca
      481 gaatgtgacg aagatgccga ggatgtctgg tgtgtgtgta atattgactg ttctcaaacc
      541 aacttcaatc ccctctgcgc ttctgatggg aaatcttatg ataatgcatg ccaaatcaaa
      601 gaagcatcgt gtcagaaaca ggagaaaatt gaagtcatgt ctttgggtcg atgtcaagat
      661 aacacaacta caactactaa gtctgaagat gggcattatg caagaacaga ttatgcagag
      721 aatgctaaca aattagaaga aagtgccaga gaacaccaca taccttgtcc ggaacattac
      781 aatggcttct gcatgcatgg gaagtgtgag cattctatca atatgcagga gccatcttgc
      841 aggtgtgatg ctggttatac tggacaacac tgtgaaaaag gtaccggagg ttccggtggt
      901 tccggtctga atgatatctt tgaagctcag aagattgaat ggcatgaagg acgtaccaag
      961 caccaccatc accatcacta a
//
