LOCUS       sequence-39942-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Pfleger BF, Fawzi NJ, Keasling JD
  TITLE     Optimization of DsRed production in Escherichia coli: effect of 
            ribosome binding site sequestration on translation efficiency.
  JOURNAL   Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
  PUBMED    16247774
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2005 Dec 5. 92(5):553-8."
COMMENT     Sequence Label: pBAD-RFP(EC)
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..750
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(206..224)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(256..276)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     474..493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     668..691
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tttttatcgc aactctctac tgtttctcca tacgtcgaca gctagcgtat tttggatgat
       61 aaggaggcgc aaaaaatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag
      121 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      181 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      241 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac
      301 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      361 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      421 ggctccttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgta
      481 atgcagaaga agactatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
      541 gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca ctacctggtg
      601 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg
      661 gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag
      721 cgcgccgagg gccgccacca cctgttcctg
//
