LOCUS       sequence-399425-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  basic plant vector with a plant-selectable kanamycin resistance 
            cassette flanked by both loxP and FRT sites for sustainable plant 
            engineering with subsequent marker removal possibility.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  TITLE     Plant vector with removable kanamycin resistance cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCambia-loxKan
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(53..73)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             complement(595..822)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    758..814
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(823..839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(823..839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(836..858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    847..863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(871..877,878..895,896..901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    916..937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1076..1123
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(1129..1162)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1234..1413
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1447..2241
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1501..1520)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2111..2130
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2281..2532
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(2556..2589)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2592..2639
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    2719..2743
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2770..2794
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3275..4069
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4645..4664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4898..4915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4930..5070
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4996..5015)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5156..5178
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5414..5608
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5674..6747)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7176..7805)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9106..9130
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(9152..9404)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9436..9453)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9460..10170)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(9500..9520)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     10115..10143
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      781 ctctagagga tccccgggta ccgagctcga attcgtaatc atgtcatagc tgtttcctgt
      841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1021 aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga cgtcagaagt
     1081 tcctattccg aagttcctat tctctagaaa gtataggaac ttcaattcat aacttcgtat
     1141 agcatacatt atacgaacgg tatgaattaa gggagtcacg ttatgacccc cgccgatgac
     1201 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca
     1261 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
     1321 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact
     1381 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgat
     1441 cgaggcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     1501 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     1561 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     1621 gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     1681 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     1741 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     1801 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     1861 catcgcatcg agcgaggacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     1921 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg
     1981 cccgacggcg aggatctcgt cgtgacccag ggcgatgcct gcttgccgaa tatcatggtg
     2041 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     2101 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     2161 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     2221 cttcttgacg agttcttctg agcgggactc tggggttcgg actctagcta gagtcaagca
     2281 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     2341 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     2401 atgacgttat ttatgagatg ggttttttga ttagagtccc gcaattatac atttaatacg
     2461 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     2521 tgttactaga tcgaccggca tgcaagctga taacctaccg ttcgtatagc atacattata
     2581 cgaagttatc tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg
     2641 ctgtccggaa cgctgagctc gaattctagt ggaggtcaaa ggagtgtcag ttaattaagt
     2701 ttaaacggcc gcaggaacgt ttacaccaca atatatcctg ccaagatcca tctcgaccgc
     2761 cgcaggaacg tttacaccac aatatatcct gccaagatct tctagtggat cccccagctt
     2821 gcatgcctgc agtagggata acagggtaat ccagccagcc aacagctccc cgaccggcag
     2881 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     2941 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     3001 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     3061 gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta
     3121 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     3181 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     3241 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     3301 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     3361 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     3421 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     3481 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     3541 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     3601 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     3661 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     3721 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     3781 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     3841 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     3901 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     3961 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     4021 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg
     4081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4261 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4921 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4981 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     5041 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     5101 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5161 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     5221 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     5281 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     5341 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     5401 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     5461 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     5521 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     5581 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     5641 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     5701 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     5761 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     5821 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     5881 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     5941 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     6001 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     6061 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     6121 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     6181 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     6241 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     6301 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     6361 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     6421 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     6481 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     6541 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     6601 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     6661 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     6721 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     6781 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     6841 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     6901 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     6961 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     7021 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
     7081 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
     7141 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
     7201 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
     7261 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
     7321 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
     7381 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
     7441 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
     7501 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
     7561 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
     7621 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
     7681 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
     7741 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
     7801 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
     7861 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
     7921 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
     7981 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
     8041 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
     8101 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
     8161 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
     8221 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
     8281 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
     8341 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
     8401 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
     8461 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
     8521 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
     8581 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
     8641 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
     8701 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
     8761 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
     8821 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
     8881 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
     8941 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
     9001 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
     9061 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
     9121 tatcctgtca aacactgata gtttaattcc cgatctagta acatagatga caccgcgcgc
     9181 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     9241 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     9301 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     9361 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggaa attcgagctg
     9421 gtcaccaatt cacacgtggt ggtggtggtg gtggctagct ttgtatagtt catccatgcc
     9481 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
     9541 gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta aaaggacagg
     9601 gccatcgcca attggagtat tttgttgata atgatcagcg agttgcacgc cgccgtcttc
     9661 gatgttgtgg cgggtcttga agttggcttt gatgccgttc ttttgcttgt cggccatgat
     9721 gtatacgttg tgggagttgt agttgtattc caacttgtgg ccgaggatgt ttccgtcctc
     9781 cttgaaatcg attcccttaa gctcgatcct gttgacgagg gtgtctccct caaacttgac
     9841 ttcagcacgt gtcttgtagt tcccgtcgtc cttgaagaag atggtcctct cctgcacgta
     9901 tccctcaggc atggcgctct tgaagaagtc gtgccgcttc atatgatctg ggtatcttga
     9961 aaagcattga acaccataag agaaagtagt gacaagtgtt ggccacggaa caggtagttt
    10021 tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc
    10081 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
    10141 aactccagtg aaaagttctt ctcctttact agtcagatct accatg
//
