LOCUS       sequence-39943-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Pfleger BF, Fawzi NJ, Keasling JD
  TITLE     Optimization of DsRed production in Escherichia coli: effect of 
            ribosome binding site sequestration on translation efficiency.
  JOURNAL   Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
  PUBMED    16247774
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2005 Dec 5. 92(5):553-8."
COMMENT     Sequence Label: pBAD*RFPEC2
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..755
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(208..226)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(258..278)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     476..495
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     670..693
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacgtcga cagctagcgt attttggatg
       61 ataaggaggc gcaaaaaatg gcgagcagtg aggacgtcat caaggagttc atgcgcttca
      121 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      181 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      241 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc
      301 accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc
      361 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      421 acggctcctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg
      481 taatgcagaa gaagactatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
      541 gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg
      601 tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg
      661 tggactccaa gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg
      721 agcgcgccga gggccgccac cacctgttcc tgtagatct
//
