LOCUS       sequence-399435-        7755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AjTn6022 proteins (except TnsA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT149: pHelper(AjTn6022)_Delta-TnsA
FEATURES             Location/Qualifiers
     source          1..7755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      5659..5706
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5877..6668)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6669..6760)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6808..7546)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg gttcagactg atactgtaag tattgaccga agtcggatca
      121 tactcaaacc aaatgtaatt gttaagtatg aagggcaacc gtataagatt gctaatgttt
      181 tgaatgccaa cgatatcgtg atttctagtt tagattctgt gcgttgttta caagtaagtg
      241 cagattctct acaagtattt gaagctgaaa ataaccaaac taaagatata aataaaggtg
      301 attgggatat tacgcatgaa gcgtggaaaa tagctttaca tcgttatgaa atcatcaagc
      361 cgttgattaa gtattcaaca accgaattag tcgagcaacg agcaagtgaa tatggcatta
      421 accgttccac cttatggaaa tggttaaaag attttagaga aaataattct atcttagtgt
      481 tagttcctaa aaagagaggt tggacgacca agaagtctag gttgtctcct caaattatga
      541 atattattaa ccaaggtatt caagaggaat atctcaatgc cagaaagcct agtattgcaa
      601 aaactataga ttttattaaa gcagaatgtt ctcgccttaa actagaagca ccccatgaca
      661 attcaattcg tagaaaaata gaagcattaa acgattatat ggtgattaag gctcgtcatg
      721 gttctaaagc agcacaagat aagtatgcat ctgctgctgg tgagtttcct aatgctgatt
      781 atcccttggc atatgtacag gtagaccaca ctccactaga tattgaaatt gttgatgatg
      841 aatttagaga ggcgattgga cgaccatttc taacattagc tattgatata aatactcgaa
      901 tggtggtcgg ttattacttg tccttagaag cccccagtgc aacatctgtt gccatgtgta
      961 ttgcttccag tattttgtca aaaaaacgga agctaattga attggatata gatgctgatt
     1021 ggcaagttga aggtcttatg gattctatac atacagataa tggtcctgac tttagaacta
     1081 accatatcag tcgagcttgc ttaaaatatg ggattcattg ggagtttcgt ccgattggtg
     1141 gggcaaggtt tggtggacat atcgaacgtt tgattggtgt tgttaacatt gaaatgcaca
     1201 tcttggatgg tacaaccttc tcgaatatcc aagaacgtgg aacctataat tcagcaggtc
     1261 atgcctgtat gacactaaaa gaactagaat attatattgt ttattggatc actaaagttt
     1321 atcaccaaaa gaaacactcc aaactcggta tgtcaccttt acagaagtgg caagatggtg
     1381 tttggggaac aaaaactagt gttggaacag gtttaaaaga gagagtttcg gatgaagata
     1441 cattatttat tgatttcctt cccgaatttg aatcaacaat tcaacgtgta ggtgtacaga
     1501 aagacaatct attttatttt gctgattgtt tacgacaatg ggttaattgt gttgatcccg
     1561 aagatgtaaa taaaaagaga aaaaagaaat ttttgtttaa acgtgatcca agggatattt
     1621 ctcttatttg gttttatgaa cctctgtcca atacatattt taaagtgcct actgctaagc
     1681 gagaaatacc accaattagc ttatttgaat acaaacaagt gcagaaatat cttcgtggcg
     1741 agcaacatga taatcaaaat caggatgaga tttatcaggc aattcttcat ttacgagagc
     1801 aattgaaaca atcccgtagt ctgactcgga agcaacggag agtaaatcaa cgtaaaaagg
     1861 aaaatgcaaa agcaacacat catttatctg taaaaaatga acctgaaaaa ccatctattg
     1921 tcgaaaacag tgttcagccg actaatgatt tatggagtat tccattgacg gcatttgaag
     1981 atttaagggg ataataatgc aaaaatatga acatcttgca gatgatgtac ttgaagtcat
     2041 gtctctaagt gatagtgatc gtatcaatat tttattttct gatcgttgga ttggttataa
     2101 aaaagcggtc actattttgg atactttaac tgatctgttg aatcgcccta ggaagcttcg
     2161 cccagaatgt ttattgattg tgggtgattc taatatgggt aaaacgacca ttatccacga
     2221 attcacaaag cagcattaca ccaaaacagt gaatgacata gatatggagc tgctgtctgt
     2281 aaaaaaacct gttttaacca tattagcccc tgctaaagcg aatgtaaaag aattctatat
     2341 caatatattg aatcatttct ttgtcccatt tcgtcctact gatcctgaag caaagttacg
     2401 gaatcaagct gtccatctaa tgcggaagta tgaaacaaaa atgttgatta ttgatgaaat
     2461 ccataattgt ttaacaggtt ctaccaaact tctttccgaa gtgatgaata ccttaaagaa
     2521 tctaagtaat gatctaagtc tgaatatcgt tggcgtcggt actcgtgaag cagttacgat
     2581 tctacatacc gatgctcaat atgcaagccg ttttgatgta gcaatgttgc ctaaatggga
     2641 actgaatcag gattttctaa gactattatt aagttatgta cgattattgc ctttaaagaa
     2701 acgttctgat ctagcatctc aaagacttgc cacccttata tttgaaattt caggtgggaa
     2761 ttttggtgac ttaaacagat tgttgattga gtgtgctaaa gaggcaatcg taaaaggtaa
     2821 tgaagaaatt accgaagaaa ttgttaataa atttaaatgg cttaaaccaa ccgagggtat
     2881 gaggaatatt agaaatataa atcttaacat ggtttaggta ttctactttt cataggaaaa
     2941 tagccatgca ccaagtagaa caacgttggt atattcgtac ccctattcaa gagggtgaag
     3001 tattttcctc ttggctcata cgttctgctt tggatttagg ctgctcacca ttaactttag
     3061 tgaatctttt gtgggaaaaa tggagaggat taacgataga tattgatcgt gggttaaatg
     3121 aagaaaaatt agacatattg ttaaatcatt gttttgtgga acagcatgaa gttcagaaaa
     3181 ctatgttaag cagttatttc cctgctttga ttccaaaaaa ttataacaaa aatcagcgta
     3241 ttccttggat attggctcta ggtattcgaa atcgttcaaa tgtgtcaggt cgtcaaattt
     3301 gtacgcaatg tctaagttct acccatacac caccatattt gcgtatcaag tggcgtatgg
     3361 ggtggcatag tggctgtgat attcataaaa ccatattaat agatcattgt cctcaatgca
     3421 gaatcacatt acaacctata aaaattgata tagagcatgg atcattagca atttgtactt
     3481 catgctatgc tgatttaagt acactcattc aacgtccagt agaaacggat gcattaagat
     3541 ttcagcatca agctgatcag gtcttagagt cacaatttgg gcgatataac caaaaggata
     3601 tttcatcaat agagtggttt gagattgctc gtatatggtt aagttttatt cgatcccctt
     3661 taaatatgaa gagtcctaac cttttgcaaa tgtttgaaag tcttaatatt gatttaaata
     3721 tagattatcc tattacacca gtagcctttg aatttctgtc tgttcaggag cgtgaaaaat
     3781 tattatcaat tttaaataaa atcatggcat taccttgtga tttatttgtt gatcgctctt
     3841 tagaatatca tgtaagtcat tcttattttt gggataaaag aaaaaaactg cctacgcagc
     3901 ttcaactaat gaaagataaa atgttgaagc caattaagat tattttgccg aaagataacg
     3961 ccatagccat gagtaaaccc aaatctaaaa aaagtgtaca acggaagtgg ttaaggctat
     4021 tgagaaagct acagaaaggg gggaacaggt gactttagct caattttccc ataaagaagg
     4081 ctgtagtggt tgtgacaggg ctgatataga taaattcaaa aaaagatata agggcgataa
     4141 gtattgttca acttgttatg cccgaatttt taaaaaaaga gcttgtccaa aatgtggtga
     4201 atatgccaga ttgccaaaaa atgatgatca agcaatttgt aatgaatgca tcaaaaaaca
     4261 gccttgtatt cgctgtaatc aaactaagaa gccgataggg acactcaccg aatatggtgt
     4321 ggtttgtaat agctgttctg tttattttcg cccaattgag cgttgtgaga ggtgtgatac
     4381 accatcacaa aaattgacac atatttcacg atttggtgat gatttaaggg tatgtcccaa
     4441 atgctctact agagattatg aaacatgtcc ctcttgtcat aaatatcgct tattagagca
     4501 agatgataca ggagctaaga tttgtaaaaa atgtaggaat aacgccaaaa agaaatgcaa
     4561 gcaatgtgat gtcttgatac cagcaggctg tcctgatcta tgtaacaact gttactggca
     4621 taagaatcta tgggagaaag ctagacgaaa tattaaagca ttccaatccc aacacttaca
     4681 ggctcaatat gagcaatatt taatttggtt agaagatgag gttggtgcaa ataaagcagc
     4741 actgtatatc aataaacata cccatttttt tatcaaaact gaagaattat ggcttgatgc
     4801 aattcctaca gcagaacagt tattggctgt tttaagaacc agtggactta gaaaatttga
     4861 gttggttgca tcttggctag atgaagccca tcacattaag gttgcattag aggataaaga
     4921 cttttgctct caacaggatc agatagaaaa actgatttca agccttcctc acccctctac
     4981 tgcttatgat gtggttatat cttataaaga tgagcttgat ataaaaatga aagatggaaa
     5041 gaccagtatc cggtccataa aactggcaat aaagcctgcg gttgctctta tgcactatgt
     5101 ttgtgcatcg ggagctacat tacctaattt aaaccatata aaagcctatc ttatcgactt
     5161 ttctggtcag gctgctgcac taacagggtt cattaatttt ttgaataaaa attttgatac
     5221 atctatagac tatttggctt ttaaaaaatc aaaaaatttc aatgaaaaac gaaaaaataa
     5281 agtagaaaaa gaaattgtcc aatgggtgga taagcctttg gagaataagg aagatgtatt
     5341 aaattgggtg aagaatgggc taagatattt tcataatgta tcttatgtgg agtctctaaa
     5401 agtcaaattt gagatgatta cagaagctga tgatggatat gaaattttat tgcagaatca
     5461 ttcttattgg ttgccaaaaa acacaggaga tttaaaaaga tagttttttt gtgattatac
     5521 tgtgctattt caagctaatt tattgtacaa gttggtttgt ttatctaatt cttctaaaag
     5581 aactgtccat aacaatatgg ggcaggctgc tgccaccgct gagcaataac ctaggctgct
     5641 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     5701 tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     5761 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
     5821 accgggtcat cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat
     5881 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     5941 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     6001 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     6061 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     6121 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     6181 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     6241 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     6301 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     6361 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     6421 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     6481 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     6541 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     6601 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     6661 tccctcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6721 agcggataca tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc
     6781 tacgctccgg gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat
     6841 tccgtggata agcaggggac taacatgtga ggcaaaacag cagggccgcg ccggtggcgt
     6901 ttttccatag gctccgccct cctgccagag ttcacataaa cagacgcttt tccggtgcat
     6961 ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact
     7021 taaagatccc caccgtttcc ggcgggtcgc tccctcttgc gctctcctgt tccgaccctg
     7081 ccgtttaccg gatacctgtt ccgcctttct cccttacggg aagtgtggcg ctttctcata
     7141 gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca
     7201 agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa
     7261 cccggaaaag cacggtaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga
     7321 ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact
     7381 ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc
     7441 ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca
     7501 aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc
     7561 gctctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     7621 atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg
     7681 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     7741 ttaattgcgt tgcgc
//
