LOCUS       sequence-399446-        4143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of ZooTsy (except TnsF) proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT156: pHelper(ZooTsy)_Delta-TnsF
FEATURES             Location/Qualifiers
     source          1..4143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      2047..2094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2265..3056)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3057..3148)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3196..3934)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg gcgaatccga agatagtgct ggcgacatcc gatgtcgctt
      121 cgcgcgctct cgcgccagca gatgccgaac tgttcgggac catcattgat gcatggactc
      181 gtcgccgcat cggctcgctg cgccataccc acaaagccgt agccagggac gtctcggtgc
      241 tgcgggagtt tgctgcccac tcaggtctgc cgccttggct ctggaatgag gatgcatttg
      301 actgctggtg cgatcatctc ggattggagc gccggttggc caatagcacc cagcggcatt
      361 accaatgctc gatccgatcc ttcctcgaat atctcacgac gaacgtcaag ttcaggaacg
      421 agatccggcg cttgtatggc gtcgagccac gacagatttg cacggaagag aactgcatcc
      481 cccataccgt cgaaaaggag tcctccaaag agcgccgagc cttcacacat gaggaaatcg
      541 agttgttttt tgacgggctg cggcgcgctt ctgtcgaggc cggccgcttc aggaccaagg
      601 attacaaccc tcttcgtcgg gaccgtactt tctttttcac cctctactcc ggagggctca
      661 ggatttccga aggcctcaac ctaaacgtaa attcgtttgc gccgaatcca gctttacctg
      721 aacttggccc ctatggattc atcaaggtct ggggcaaggg aagccgggga agtgggaagc
      781 gctttcggac cgtaccggtc acccatcctg acttccctga gctcatggac tggtacatca
      841 caagagtccg ccctgaattc cttcatcgag cggaccccaa tgaggaagcc ttgttcctct
      901 cggagcgggg aactcgtctc aagtcgtcca gcgccgacgc acgcttcaaa atcgcaactc
      961 gctattgcgg cctggaaggc gaaggactga cgccacactg tctgcgccac tcgagcgtta
     1021 cccacgaatc cttgcgcttc agcatcgaga tggttcgtcg gaagcatggc cacgcttctg
     1081 cggcaacgac acaaggctat atgcacgtcc ccgacgttat ggtcgacgac gaaatcagca
     1141 gcgtcgtctc ccgcgagctc gatgacgcgc tcaacacaca ggagaagaaa gaatgagcga
     1201 tacgcccaat atcagacttg gttggcgcct ccgcgtgcgg atggcggaaa accgaatacc
     1261 gaccgccacg gaactcaagc gacggctcga cgcgatcggc tacgacatca ccagcgccca
     1321 actctcccga attgtggacg agcgaccggc ccaggtgaaa accgcgctac tggaggcttt
     1381 gcttaaagtc ctggggggaa ccctagacga tctcatgccc gcggagggcc gtgttgagca
     1441 gccgagttct gcgcctcaag gtggtgagga agttgcgcca agcccagcgc cggcggccac
     1501 ccccacccgt aagcgcaaac cggcgcgccc cgcctcggaa gcagtcgatc cggacgatgc
     1561 cggtggcccc agggtacgcc ccttctcggt cccaccgaag aagtgaaata gcgttcctac
     1621 atggctggcg aacttaccgt tcggaaaaat ccatacgcca gcggtcagcc gtggtggctc
     1681 tatatgctcg aatgcaaggg cggaggcatc tacaccggca tcgcgctgga tgtggatgcg
     1741 cggtttgagc aacacgtcgc aggcaagggc gccaaataca cgcgcatgaa ccggcccatc
     1801 cgggtcgtcg cgcgcatccg ctacgacacg caccgggaag ccgctcaggc tgaagttgcg
     1861 ctgaaacgcc tcaagcccct ggaaaagctg cggtgggcct atgccttggc tgggttagtc
     1921 ggcatggaca agtcggcgta acgcgtgggg cgtctccgga tcgttgaggg gcaaaagcat
     1981 cgcggccccc gaggctgctg ccaccgctga gcaataacct aggctgctgc caccgctgag
     2041 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     2101 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     2161 cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcatcg
     2221 tggccggatc ttgcggcccc tcggcttgaa cgaattgtta gacattattt gccgactacc
     2281 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
     2341 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
     2401 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
     2461 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
     2521 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
     2581 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
     2641 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
     2701 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     2761 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     2821 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     2881 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     2941 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     3001 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc
     3061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3121 tttgaatgta tttagaaaaa taaacaaata gctagctcac tcggtcgcta cgctccgggc
     3181 gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag
     3241 caggggacta acatgtgagg caaaacagca gggccgcgcc ggtggcgttt ttccataggc
     3301 tccgccctcc tgccagagtt cacataaaca gacgcttttc cggtgcatct gtgggagccg
     3361 tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca
     3421 ccgtttccgg cgggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga
     3481 tacctgttcc gcctttctcc cttacgggaa gtgtggcgct ttctcatagc tcacacactg
     3541 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt
     3601 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca
     3661 cggtaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     3721 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     3781 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     3841 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     3901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc tctagatttc
     3961 agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta
     4021 attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     4081 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     4141 cgc
//
