LOCUS       sequence-399447-        7546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AjTn6022 proteins (except TnsC)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT151: pHelper(AjTn6022)_Delta-TnsC
FEATURES             Location/Qualifiers
     source          1..7546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      5450..5497
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5668..6459)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6460..6551)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6599..7337)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg aaaagaatca gtgatgaata ttccccagtt aggaaatccc
      121 aaactgtata tgggagtatt tcagtaaatt atgcttttag aggggaaaag acgatttggt
      181 gtgaatctac tcttgagcgt gattttttga ttaggcaaga attcaatagc aatgtcatag
      241 atatcgtaag ccagcctatt accatcccat atataacaga gttgggtaat gaatccactt
      301 atactcctga tttcctcgta cagttttcca gtgcaaattg tggtgatttt gaggaaattc
      361 cctgtccact gttaatagaa ataaaaccta gaaagaaact tatagaagat tgggataaat
      421 taaagccaaa atttaaggcg gctcatactt ttgctaaaga aaaaggttgg aaattcaaga
      481 ttataaatga atcaaggtta tatgatcagt attgggaaaa tattaatttt ttgaaaagat
      541 ttagacgttc tcatgtggat gtctatgatg aacaaacttt aattgagact ttagatagtc
      601 ttggtcaatc gactattaat caattaccgg cacatatttt taaggataaa actaatattc
      661 ttcgaggtat tcatcaagta tgggcattgg tcgcaaaacg aaaaatcatg tgtgatttat
      721 attgcccctt aacagcagag acggtgattt ggacaaatca agatgatgtg cttacaagga
      781 gatttttaaa tggttcagac tgatactgta agtattgacc gaagtcggat catactcaaa
      841 ccaaatgtaa ttgttaagta tgaagggcaa ccgtataaga ttgctaatgt tttgaatgcc
      901 aacgatatcg tgatttctag tttagattct gtgcgttgtt tacaagtaag tgcagattct
      961 ctacaagtat ttgaagctga aaataaccaa actaaagata taaataaagg tgattgggat
     1021 attacgcatg aagcgtggaa aatagcttta catcgttatg aaatcatcaa gccgttgatt
     1081 aagtattcaa caaccgaatt agtcgagcaa cgagcaagtg aatatggcat taaccgttcc
     1141 accttatgga aatggttaaa agattttaga gaaaataatt ctatcttagt gttagttcct
     1201 aaaaagagag gttggacgac caagaagtct aggttgtctc ctcaaattat gaatattatt
     1261 aaccaaggta ttcaagagga atatctcaat gccagaaagc ctagtattgc aaaaactata
     1321 gattttatta aagcagaatg ttctcgcctt aaactagaag caccccatga caattcaatt
     1381 cgtagaaaaa tagaagcatt aaacgattat atggtgatta aggctcgtca tggttctaaa
     1441 gcagcacaag ataagtatgc atctgctgct ggtgagtttc ctaatgctga ttatcccttg
     1501 gcatatgtac aggtagacca cactccacta gatattgaaa ttgttgatga tgaatttaga
     1561 gaggcgattg gacgaccatt tctaacatta gctattgata taaatactcg aatggtggtc
     1621 ggttattact tgtccttaga agcccccagt gcaacatctg ttgccatgtg tattgcttcc
     1681 agtattttgt caaaaaaacg gaagctaatt gaattggata tagatgctga ttggcaagtt
     1741 gaaggtctta tggattctat acatacagat aatggtcctg actttagaac taaccatatc
     1801 agtcgagctt gcttaaaata tgggattcat tgggagtttc gtccgattgg tggggcaagg
     1861 tttggtggac atatcgaacg tttgattggt gttgttaaca ttgaaatgca catcttggat
     1921 ggtacaacct tctcgaatat ccaagaacgt ggaacctata attcagcagg tcatgcctgt
     1981 atgacactaa aagaactaga atattatatt gtttattgga tcactaaagt ttatcaccaa
     2041 aagaaacact ccaaactcgg tatgtcacct ttacagaagt ggcaagatgg tgtttgggga
     2101 acaaaaacta gtgttggaac aggtttaaaa gagagagttt cggatgaaga tacattattt
     2161 attgatttcc ttcccgaatt tgaatcaaca attcaacgtg taggtgtaca gaaagacaat
     2221 ctattttatt ttgctgattg tttacgacaa tgggttaatt gtgttgatcc cgaagatgta
     2281 aataaaaaga gaaaaaagaa atttttgttt aaacgtgatc caagggatat ttctcttatt
     2341 tggttttatg aacctctgtc caatacatat tttaaagtgc ctactgctaa gcgagaaata
     2401 ccaccaatta gcttatttga atacaaacaa gtgcagaaat atcttcgtgg cgagcaacat
     2461 gataatcaaa atcaggatga gatttatcag gcaattcttc atttacgaga gcaattgaaa
     2521 caatcccgta gtctgactcg gaagcaacgg agagtaaatc aacgtaaaaa ggaaaatgca
     2581 aaagcaacac atcatttatc tgtaaaaaat gaacctgaaa aaccatctat tgtcgaaaac
     2641 agtgttcagc cgactaatga tttatggagt attccattga cggcatttga agatttaagg
     2701 ggataatagt attctacttt tcataggaaa atagccatgc accaagtaga acaacgttgg
     2761 tatattcgta cccctattca agagggtgaa gtattttcct cttggctcat acgttctgct
     2821 ttggatttag gctgctcacc attaacttta gtgaatcttt tgtgggaaaa atggagagga
     2881 ttaacgatag atattgatcg tgggttaaat gaagaaaaat tagacatatt gttaaatcat
     2941 tgttttgtgg aacagcatga agttcagaaa actatgttaa gcagttattt ccctgctttg
     3001 attccaaaaa attataacaa aaatcagcgt attccttgga tattggctct aggtattcga
     3061 aatcgttcaa atgtgtcagg tcgtcaaatt tgtacgcaat gtctaagttc tacccataca
     3121 ccaccatatt tgcgtatcaa gtggcgtatg gggtggcata gtggctgtga tattcataaa
     3181 accatattaa tagatcattg tcctcaatgc agaatcacat tacaacctat aaaaattgat
     3241 atagagcatg gatcattagc aatttgtact tcatgctatg ctgatttaag tacactcatt
     3301 caacgtccag tagaaacgga tgcattaaga tttcagcatc aagctgatca ggtcttagag
     3361 tcacaatttg ggcgatataa ccaaaaggat atttcatcaa tagagtggtt tgagattgct
     3421 cgtatatggt taagttttat tcgatcccct ttaaatatga agagtcctaa ccttttgcaa
     3481 atgtttgaaa gtcttaatat tgatttaaat atagattatc ctattacacc agtagccttt
     3541 gaatttctgt ctgttcagga gcgtgaaaaa ttattatcaa ttttaaataa aatcatggca
     3601 ttaccttgtg atttatttgt tgatcgctct ttagaatatc atgtaagtca ttcttatttt
     3661 tgggataaaa gaaaaaaact gcctacgcag cttcaactaa tgaaagataa aatgttgaag
     3721 ccaattaaga ttattttgcc gaaagataac gccatagcca tgagtaaacc caaatctaaa
     3781 aaaagtgtac aacggaagtg gttaaggcta ttgagaaagc tacagaaagg ggggaacagg
     3841 tgactttagc tcaattttcc cataaagaag gctgtagtgg ttgtgacagg gctgatatag
     3901 ataaattcaa aaaaagatat aagggcgata agtattgttc aacttgttat gcccgaattt
     3961 ttaaaaaaag agcttgtcca aaatgtggtg aatatgccag attgccaaaa aatgatgatc
     4021 aagcaatttg taatgaatgc atcaaaaaac agccttgtat tcgctgtaat caaactaaga
     4081 agccgatagg gacactcacc gaatatggtg tggtttgtaa tagctgttct gtttattttc
     4141 gcccaattga gcgttgtgag aggtgtgata caccatcaca aaaattgaca catatttcac
     4201 gatttggtga tgatttaagg gtatgtccca aatgctctac tagagattat gaaacatgtc
     4261 cctcttgtca taaatatcgc ttattagagc aagatgatac aggagctaag atttgtaaaa
     4321 aatgtaggaa taacgccaaa aagaaatgca agcaatgtga tgtcttgata ccagcaggct
     4381 gtcctgatct atgtaacaac tgttactggc ataagaatct atgggagaaa gctagacgaa
     4441 atattaaagc attccaatcc caacacttac aggctcaata tgagcaatat ttaatttggt
     4501 tagaagatga ggttggtgca aataaagcag cactgtatat caataaacat acccattttt
     4561 ttatcaaaac tgaagaatta tggcttgatg caattcctac agcagaacag ttattggctg
     4621 ttttaagaac cagtggactt agaaaatttg agttggttgc atcttggcta gatgaagccc
     4681 atcacattaa ggttgcatta gaggataaag acttttgctc tcaacaggat cagatagaaa
     4741 aactgatttc aagccttcct cacccctcta ctgcttatga tgtggttata tcttataaag
     4801 atgagcttga tataaaaatg aaagatggaa agaccagtat ccggtccata aaactggcaa
     4861 taaagcctgc ggttgctctt atgcactatg tttgtgcatc gggagctaca ttacctaatt
     4921 taaaccatat aaaagcctat cttatcgact tttctggtca ggctgctgca ctaacagggt
     4981 tcattaattt tttgaataaa aattttgata catctataga ctatttggct tttaaaaaat
     5041 caaaaaattt caatgaaaaa cgaaaaaata aagtagaaaa agaaattgtc caatgggtgg
     5101 ataagccttt ggagaataag gaagatgtat taaattgggt gaagaatggg ctaagatatt
     5161 ttcataatgt atcttatgtg gagtctctaa aagtcaaatt tgagatgatt acagaagctg
     5221 atgatggata tgaaatttta ttgcagaatc attcttattg gttgccaaaa aacacaggag
     5281 atttaaaaag atagtttttt tgtgattata ctgtgctatt tcaagctaat ttattgtaca
     5341 agttggtttg tttatctaat tcttctaaaa gaactgtcca taacaatatg gggcaggctg
     5401 ctgccaccgc tgagcaataa cctaggctgc tgccaccgct gagcaataac tagcataacc
     5461 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
     5521 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     5581 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc
     5641 ccctcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
     5701 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     5761 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     5821 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     5881 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     5941 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     6001 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     6061 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     6121 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     6181 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     6241 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     6301 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     6361 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     6421 tagttgagtc gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta
     6481 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6541 aaataaacaa atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc
     6601 gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg
     6661 aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga
     6721 gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa
     6781 tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg
     6841 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
     6901 tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
     6961 aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
     7021 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
     7081 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
     7141 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
     7201 cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
     7261 ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctactgaac cgctctagat ttcagtgcaa tttatctctt
     7381 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
     7441 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     7501 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgc
//
