LOCUS       sequence-399449-        5556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of ZooTsy (except HTH) proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT155: pHelper(ZooTsy)_Delta-HTH
FEATURES             Location/Qualifiers
     source          1..5556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      3460..3507
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3678..4469)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4470..4561)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4609..5347)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg gcgaatccga agatagtgct ggcgacatcc gatgtcgctt
      121 cgcgcgctct cgcgccagca gatgccgaac tgttcgggac catcattgat gcatggactc
      181 gtcgccgcat cggctcgctg cgccataccc acaaagccgt agccagggac gtctcggtgc
      241 tgcgggagtt tgctgcccac tcaggtctgc cgccttggct ctggaatgag gatgcatttg
      301 actgctggtg cgatcatctc ggattggagc gccggttggc caatagcacc cagcggcatt
      361 accaatgctc gatccgatcc ttcctcgaat atctcacgac gaacgtcaag ttcaggaacg
      421 agatccggcg cttgtatggc gtcgagccac gacagatttg cacggaagag aactgcatcc
      481 cccataccgt cgaaaaggag tcctccaaag agcgccgagc cttcacacat gaggaaatcg
      541 agttgttttt tgacgggctg cggcgcgctt ctgtcgaggc cggccgcttc aggaccaagg
      601 attacaaccc tcttcgtcgg gaccgtactt tctttttcac cctctactcc ggagggctca
      661 ggatttccga aggcctcaac ctaaacgtaa attcgtttgc gccgaatcca gctttacctg
      721 aacttggccc ctatggattc atcaaggtct ggggcaaggg aagccgggga agtgggaagc
      781 gctttcggac cgtaccggtc acccatcctg acttccctga gctcatggac tggtacatca
      841 caagagtccg ccctgaattc cttcatcgag cggaccccaa tgaggaagcc ttgttcctct
      901 cggagcgggg aactcgtctc aagtcgtcca gcgccgacgc acgcttcaaa atcgcaactc
      961 gctattgcgg cctggaaggc gaaggactga cgccacactg tctgcgccac tcgagcgtta
     1021 cccacgaatc cttgcgcttc agcatcgaga tggttcgtcg gaagcatggc cacgcttctg
     1081 cggcaacgac acaaggctat atgcacgtcc ccgacgttat ggtcgacgac gaaatcagca
     1141 gcgtcgtctc ccgcgagctc gatgacgcgc tcaacacaca ggagaagaaa gaatgaaata
     1201 gcgttcctac atggcgacct ctgctcatac ctgttgcgat gaatgcagcc gtccgatggc
     1261 caaggcgcgt ctcgtccgga acgggaaggc cttgtgcgac acctgcagcc agcggattcc
     1321 ctacgcccct tgtggcaagt gcggaaagca gacgcgcatg ctcaatgacc aaggggaacc
     1381 gctctgcaag gtctgccgtg ccctcaaccg cacctgcgtg cgttgcggca agccagtgcg
     1441 caaggctggg cgcttggtcg atggcggtgc tgtctgcggc ccctgcgcca agtattaccg
     1501 tgaaccgcag gcctgcgatc aatgtggggc aatggagaag gttcttaccg gcgtacagac
     1561 agaaactggg cggcgccgtc tctgcggcag gtgtttccgg cgccataaag gctacaagag
     1621 ctgcagcatc tgccggaaag acaggctgcc tgtcggcgtg accgctgacg gcaagcctat
     1681 atgcaaaacc tgtttgagtg cggccggaaa acctttcgtg tgcccaacct gtggccagga
     1741 aggaatacgc catagtagaa ggcgttgcca gaagtgctac gttcgcgata cggcgaaaag
     1801 ccgtgtcgag gaatatgctc ccctttttag tcatgcatgg acccgggaag cctggctggg
     1861 atttggtgca gagaccctgg aggcaatgaa tccagaaaag ctagtgcatc gaatcaacag
     1921 ctactacctc ttcttcgtcc ggcttgatct cgccttcagc aaacctacag aaatcacgcc
     1981 ggtcgcgctg ttgaaaagcg tcggcggctt ggatgggctc cgtcgataca ccgtgcccta
     2041 tggctatctc ctgaagcacc gaatcatccc cgagacaacg cgcagtctcc tgcaggacga
     2101 ggctgaatcc atcaggcagg aagccatgat cgccaagctg gatggcaagt ggtatgcccc
     2161 agtcatggca cgctaccgga atcacctgct gactgtccgc gcccgatacg acagccgggg
     2221 ctggaaaggg gaagataccc ggatgaaggc gcgctcgatt acgctcaacc tccgggcggc
     2281 tcggcgttta tgcgaaacgc tggatgccat aggtgtccag atgattcagc agacgacgcc
     2341 agagatgctg gacaagttct tcatggacta tcctggactg gccgtctcaa tccgtgcctt
     2401 cgtccgatac ctgaaccgga aggaaaaact ctttcagcgc ctgcgcctga agacagttgc
     2461 ccggagtgtg ccggaaggga ttttcctgcc ccgcgaaaaa tccactgcgc tcattcaagc
     2521 gtggatgacg ccgactgatg agacgcttcg gcagtccctc atcggcctgt tcatgctgct
     2581 ctatgcccag acggtcaaga actgtgttcg gcttcgtctg gatgcactca tccaacgggc
     2641 tgacgggcgt tatcgcatag catttggccg cgcggaaatc cacctggatg ctcggatcag
     2701 cgcgatactc gatcgctatt tgaacgtgcg gcaaacactg gttgctctgg accatgccga
     2761 cgaaaatccg tatctctttc cgggacggca gttaggcagt cacctgagtg ccgaatcggt
     2821 atccggttgg ttgaaaaagg cgggcgtcag ctccgatcag ttgtttgcaa catcaatcta
     2881 taacgcctat caaaatggcc tgcgacttcc caaggtcttg gtgcgagcgt ttggcatcac
     2941 cgatacgacc gcgatcaagt atctcaacat gctggatccc cgcctggtca tggagatcga
     3001 acgcaaacca ctgggcggcg gggacgaacc ctgatggctg gcgaacttac cgttcggaaa
     3061 aatccatacg ccagcggtca gccgtggtgg ctctatatgc tcgaatgcaa gggcggaggc
     3121 atctacaccg gcatcgcgct ggatgtggat gcgcggtttg agcaacacgt cgcaggcaag
     3181 ggcgccaaat acacgcgcat gaaccggccc atccgggtcg tcgcgcgcat ccgctacgac
     3241 acgcaccggg aagccgctca ggctgaagtt gcgctgaaac gcctcaagcc cctggaaaag
     3301 ctgcggtggg cctatgcctt ggctgggtta gtcggcatgg acaagtcggc gtaacgcgtg
     3361 gggcgtctcc ggatcgttga ggggcaaaag catcgcggcc cccgaggctg ctgccaccgc
     3421 tgagcaataa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     3481 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
     3541 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     3601 cgaccctgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt
     3661 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     3721 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     3781 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     3841 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     3901 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     3961 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     4021 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     4081 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     4141 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     4201 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     4261 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     4321 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     4381 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     4441 gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt
     4501 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4561 atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca
     4621 catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca
     4681 gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa
     4741 acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta
     4801 cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg
     4861 cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg
     4921 gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg
     4981 ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg
     5041 taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat
     5101 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     5161 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     5221 ttaccacggt taagcagttc cccaactgnc ttaaccttcg atcaaaccac ctccccaggt
     5281 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     5341 tttgatcttt tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc
     5401 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc
     5461 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     5521 taatgagtga gctaacttac attaattgcg ttgcgc
//
