LOCUS       sequence-399452-        5966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of ZooTsy proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT148: pHelper(ZooTsy)
FEATURES             Location/Qualifiers
     source          1..5966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      3870..3917
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4088..4879)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(4880..4971)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5019..5757)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg gcgaatccga agatagtgct ggcgacatcc gatgtcgctt
      121 cgcgcgctct cgcgccagca gatgccgaac tgttcgggac catcattgat gcatggactc
      181 gtcgccgcat cggctcgctg cgccataccc acaaagccgt agccagggac gtctcggtgc
      241 tgcgggagtt tgctgcccac tcaggtctgc cgccttggct ctggaatgag gatgcatttg
      301 actgctggtg cgatcatctc ggattggagc gccggttggc caatagcacc cagcggcatt
      361 accaatgctc gatccgatcc ttcctcgaat atctcacgac gaacgtcaag ttcaggaacg
      421 agatccggcg cttgtatggc gtcgagccac gacagatttg cacggaagag aactgcatcc
      481 cccataccgt cgaaaaggag tcctccaaag agcgccgagc cttcacacat gaggaaatcg
      541 agttgttttt tgacgggctg cggcgcgctt ctgtcgaggc cggccgcttc aggaccaagg
      601 attacaaccc tcttcgtcgg gaccgtactt tctttttcac cctctactcc ggagggctca
      661 ggatttccga aggcctcaac ctaaacgtaa attcgtttgc gccgaatcca gctttacctg
      721 aacttggccc ctatggattc atcaaggtct ggggcaaggg aagccgggga agtgggaagc
      781 gctttcggac cgtaccggtc acccatcctg acttccctga gctcatggac tggtacatca
      841 caagagtccg ccctgaattc cttcatcgag cggaccccaa tgaggaagcc ttgttcctct
      901 cggagcgggg aactcgtctc aagtcgtcca gcgccgacgc acgcttcaaa atcgcaactc
      961 gctattgcgg cctggaaggc gaaggactga cgccacactg tctgcgccac tcgagcgtta
     1021 cccacgaatc cttgcgcttc agcatcgaga tggttcgtcg gaagcatggc cacgcttctg
     1081 cggcaacgac acaaggctat atgcacgtcc ccgacgttat ggtcgacgac gaaatcagca
     1141 gcgtcgtctc ccgcgagctc gatgacgcgc tcaacacaca ggagaagaaa gaatgagcga
     1201 tacgcccaat atcagacttg gttggcgcct ccgcgtgcgg atggcggaaa accgaatacc
     1261 gaccgccacg gaactcaagc gacggctcga cgcgatcggc tacgacatca ccagcgccca
     1321 actctcccga attgtggacg agcgaccggc ccaggtgaaa accgcgctac tggaggcttt
     1381 gcttaaagtc ctggggggaa ccctagacga tctcatgccc gcggagggcc gtgttgagca
     1441 gccgagttct gcgcctcaag gtggtgagga agttgcgcca agcccagcgc cggcggccac
     1501 ccccacccgt aagcgcaaac cggcgcgccc cgcctcggaa gcagtcgatc cggacgatgc
     1561 cggtggcccc agggtacgcc ccttctcggt cccaccgaag aagtgaaata gcgttcctac
     1621 atggcgacct ctgctcatac ctgttgcgat gaatgcagcc gtccgatggc caaggcgcgt
     1681 ctcgtccgga acgggaaggc cttgtgcgac acctgcagcc agcggattcc ctacgcccct
     1741 tgtggcaagt gcggaaagca gacgcgcatg ctcaatgacc aaggggaacc gctctgcaag
     1801 gtctgccgtg ccctcaaccg cacctgcgtg cgttgcggca agccagtgcg caaggctggg
     1861 cgcttggtcg atggcggtgc tgtctgcggc ccctgcgcca agtattaccg tgaaccgcag
     1921 gcctgcgatc aatgtggggc aatggagaag gttcttaccg gcgtacagac agaaactggg
     1981 cggcgccgtc tctgcggcag gtgtttccgg cgccataaag gctacaagag ctgcagcatc
     2041 tgccggaaag acaggctgcc tgtcggcgtg accgctgacg gcaagcctat atgcaaaacc
     2101 tgtttgagtg cggccggaaa acctttcgtg tgcccaacct gtggccagga aggaatacgc
     2161 catagtagaa ggcgttgcca gaagtgctac gttcgcgata cggcgaaaag ccgtgtcgag
     2221 gaatatgctc ccctttttag tcatgcatgg acccgggaag cctggctggg atttggtgca
     2281 gagaccctgg aggcaatgaa tccagaaaag ctagtgcatc gaatcaacag ctactacctc
     2341 ttcttcgtcc ggcttgatct cgccttcagc aaacctacag aaatcacgcc ggtcgcgctg
     2401 ttgaaaagcg tcggcggctt ggatgggctc cgtcgataca ccgtgcccta tggctatctc
     2461 ctgaagcacc gaatcatccc cgagacaacg cgcagtctcc tgcaggacga ggctgaatcc
     2521 atcaggcagg aagccatgat cgccaagctg gatggcaagt ggtatgcccc agtcatggca
     2581 cgctaccgga atcacctgct gactgtccgc gcccgatacg acagccgggg ctggaaaggg
     2641 gaagataccc ggatgaaggc gcgctcgatt acgctcaacc tccgggcggc tcggcgttta
     2701 tgcgaaacgc tggatgccat aggtgtccag atgattcagc agacgacgcc agagatgctg
     2761 gacaagttct tcatggacta tcctggactg gccgtctcaa tccgtgcctt cgtccgatac
     2821 ctgaaccgga aggaaaaact ctttcagcgc ctgcgcctga agacagttgc ccggagtgtg
     2881 ccggaaggga ttttcctgcc ccgcgaaaaa tccactgcgc tcattcaagc gtggatgacg
     2941 ccgactgatg agacgcttcg gcagtccctc atcggcctgt tcatgctgct ctatgcccag
     3001 acggtcaaga actgtgttcg gcttcgtctg gatgcactca tccaacgggc tgacgggcgt
     3061 tatcgcatag catttggccg cgcggaaatc cacctggatg ctcggatcag cgcgatactc
     3121 gatcgctatt tgaacgtgcg gcaaacactg gttgctctgg accatgccga cgaaaatccg
     3181 tatctctttc cgggacggca gttaggcagt cacctgagtg ccgaatcggt atccggttgg
     3241 ttgaaaaagg cgggcgtcag ctccgatcag ttgtttgcaa catcaatcta taacgcctat
     3301 caaaatggcc tgcgacttcc caaggtcttg gtgcgagcgt ttggcatcac cgatacgacc
     3361 gcgatcaagt atctcaacat gctggatccc cgcctggtca tggagatcga acgcaaacca
     3421 ctgggcggcg gggacgaacc ctgatggctg gcgaacttac cgttcggaaa aatccatacg
     3481 ccagcggtca gccgtggtgg ctctatatgc tcgaatgcaa gggcggaggc atctacaccg
     3541 gcatcgcgct ggatgtggat gcgcggtttg agcaacacgt cgcaggcaag ggcgccaaat
     3601 acacgcgcat gaaccggccc atccgggtcg tcgcgcgcat ccgctacgac acgcaccggg
     3661 aagccgctca ggctgaagtt gcgctgaaac gcctcaagcc cctggaaaag ctgcggtggg
     3721 cctatgcctt ggctgggtta gtcggcatgg acaagtcggc gtaacgcgtg gggcgtctcc
     3781 ggatcgttga ggggcaaaag catcgcggcc cccgaggctg ctgccaccgc tgagcaataa
     3841 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     3901 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     3961 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
     4021 ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg
     4081 ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     4141 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     4201 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     4261 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     4321 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     4381 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     4441 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     4501 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     4561 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     4621 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     4681 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     4741 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     4801 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     4861 cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct
     4981 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
     5041 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
     5101 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
     5161 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
     5221 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
     5281 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
     5341 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     5401 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
     5461 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
     5521 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
     5581 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
     5641 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
     5701 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5761 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
     5821 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
     5881 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     5941 gctaacttac attaattgcg ttgcgc
//
