LOCUS       sequence-399453-        6564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AjTn6022 proteins (except TnsB)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT150: pHelper(AjTn6022)_Delta-TnsB
FEATURES             Location/Qualifiers
     source          1..6564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      4468..4515
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4686..5477)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(5478..5569)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5617..6355)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg aaaagaatca gtgatgaata ttccccagtt aggaaatccc
      121 aaactgtata tgggagtatt tcagtaaatt atgcttttag aggggaaaag acgatttggt
      181 gtgaatctac tcttgagcgt gattttttga ttaggcaaga attcaatagc aatgtcatag
      241 atatcgtaag ccagcctatt accatcccat atataacaga gttgggtaat gaatccactt
      301 atactcctga tttcctcgta cagttttcca gtgcaaattg tggtgatttt gaggaaattc
      361 cctgtccact gttaatagaa ataaaaccta gaaagaaact tatagaagat tgggataaat
      421 taaagccaaa atttaaggcg gctcatactt ttgctaaaga aaaaggttgg aaattcaaga
      481 ttataaatga atcaaggtta tatgatcagt attgggaaaa tattaatttt ttgaaaagat
      541 ttagacgttc tcatgtggat gtctatgatg aacaaacttt aattgagact ttagatagtc
      601 ttggtcaatc gactattaat caattaccgg cacatatttt taaggataaa actaatattc
      661 ttcgaggtat tcatcaagta tgggcattgg tcgcaaaacg aaaaatcatg tgtgatttat
      721 attgcccctt aacagcagag acggtgattt ggacaaatca agatgatgtg cttacaagga
      781 gatttttaaa tggttcagac tgataatgca aaaatatgaa catcttgcag atgatgtact
      841 tgaagtcatg tctctaagtg atagtgatcg tatcaatatt ttattttctg atcgttggat
      901 tggttataaa aaagcggtca ctattttgga tactttaact gatctgttga atcgccctag
      961 gaagcttcgc ccagaatgtt tattgattgt gggtgattct aatatgggta aaacgaccat
     1021 tatccacgaa ttcacaaagc agcattacac caaaacagtg aatgacatag atatggagct
     1081 gctgtctgta aaaaaacctg ttttaaccat attagcccct gctaaagcga atgtaaaaga
     1141 attctatatc aatatattga atcatttctt tgtcccattt cgtcctactg atcctgaagc
     1201 aaagttacgg aatcaagctg tccatctaat gcggaagtat gaaacaaaaa tgttgattat
     1261 tgatgaaatc cataattgtt taacaggttc taccaaactt ctttccgaag tgatgaatac
     1321 cttaaagaat ctaagtaatg atctaagtct gaatatcgtt ggcgtcggta ctcgtgaagc
     1381 agttacgatt ctacataccg atgctcaata tgcaagccgt tttgatgtag caatgttgcc
     1441 taaatgggaa ctgaatcagg attttctaag actattatta agttatgtac gattattgcc
     1501 tttaaagaaa cgttctgatc tagcatctca aagacttgcc acccttatat ttgaaatttc
     1561 aggtgggaat tttggtgact taaacagatt gttgattgag tgtgctaaag aggcaatcgt
     1621 aaaaggtaat gaagaaatta ccgaagaaat tgttaataaa tttaaatggc ttaaaccaac
     1681 cgagggtatg aggaatatta gaaatataaa tcttaacatg gtttaggtat tctacttttc
     1741 ataggaaaat agccatgcac caagtagaac aacgttggta tattcgtacc cctattcaag
     1801 agggtgaagt attttcctct tggctcatac gttctgcttt ggatttaggc tgctcaccat
     1861 taactttagt gaatcttttg tgggaaaaat ggagaggatt aacgatagat attgatcgtg
     1921 ggttaaatga agaaaaatta gacatattgt taaatcattg ttttgtggaa cagcatgaag
     1981 ttcagaaaac tatgttaagc agttatttcc ctgctttgat tccaaaaaat tataacaaaa
     2041 atcagcgtat tccttggata ttggctctag gtattcgaaa tcgttcaaat gtgtcaggtc
     2101 gtcaaatttg tacgcaatgt ctaagttcta cccatacacc accatatttg cgtatcaagt
     2161 ggcgtatggg gtggcatagt ggctgtgata ttcataaaac catattaata gatcattgtc
     2221 ctcaatgcag aatcacatta caacctataa aaattgatat agagcatgga tcattagcaa
     2281 tttgtacttc atgctatgct gatttaagta cactcattca acgtccagta gaaacggatg
     2341 cattaagatt tcagcatcaa gctgatcagg tcttagagtc acaatttggg cgatataacc
     2401 aaaaggatat ttcatcaata gagtggtttg agattgctcg tatatggtta agttttattc
     2461 gatccccttt aaatatgaag agtcctaacc ttttgcaaat gtttgaaagt cttaatattg
     2521 atttaaatat agattatcct attacaccag tagcctttga atttctgtct gttcaggagc
     2581 gtgaaaaatt attatcaatt ttaaataaaa tcatggcatt accttgtgat ttatttgttg
     2641 atcgctcttt agaatatcat gtaagtcatt cttatttttg ggataaaaga aaaaaactgc
     2701 ctacgcagct tcaactaatg aaagataaaa tgttgaagcc aattaagatt attttgccga
     2761 aagataacgc catagccatg agtaaaccca aatctaaaaa aagtgtacaa cggaagtggt
     2821 taaggctatt gagaaagcta cagaaagggg ggaacaggtg actttagctc aattttccca
     2881 taaagaaggc tgtagtggtt gtgacagggc tgatatagat aaattcaaaa aaagatataa
     2941 gggcgataag tattgttcaa cttgttatgc ccgaattttt aaaaaaagag cttgtccaaa
     3001 atgtggtgaa tatgccagat tgccaaaaaa tgatgatcaa gcaatttgta atgaatgcat
     3061 caaaaaacag ccttgtattc gctgtaatca aactaagaag ccgataggga cactcaccga
     3121 atatggtgtg gtttgtaata gctgttctgt ttattttcgc ccaattgagc gttgtgagag
     3181 gtgtgataca ccatcacaaa aattgacaca tatttcacga tttggtgatg atttaagggt
     3241 atgtcccaaa tgctctacta gagattatga aacatgtccc tcttgtcata aatatcgctt
     3301 attagagcaa gatgatacag gagctaagat ttgtaaaaaa tgtaggaata acgccaaaaa
     3361 gaaatgcaag caatgtgatg tcttgatacc agcaggctgt cctgatctat gtaacaactg
     3421 ttactggcat aagaatctat gggagaaagc tagacgaaat attaaagcat tccaatccca
     3481 acacttacag gctcaatatg agcaatattt aatttggtta gaagatgagg ttggtgcaaa
     3541 taaagcagca ctgtatatca ataaacatac ccattttttt atcaaaactg aagaattatg
     3601 gcttgatgca attcctacag cagaacagtt attggctgtt ttaagaacca gtggacttag
     3661 aaaatttgag ttggttgcat cttggctaga tgaagcccat cacattaagg ttgcattaga
     3721 ggataaagac ttttgctctc aacaggatca gatagaaaaa ctgatttcaa gccttcctca
     3781 cccctctact gcttatgatg tggttatatc ttataaagat gagcttgata taaaaatgaa
     3841 agatggaaag accagtatcc ggtccataaa actggcaata aagcctgcgg ttgctcttat
     3901 gcactatgtt tgtgcatcgg gagctacatt acctaattta aaccatataa aagcctatct
     3961 tatcgacttt tctggtcagg ctgctgcact aacagggttc attaattttt tgaataaaaa
     4021 ttttgataca tctatagact atttggcttt taaaaaatca aaaaatttca atgaaaaacg
     4081 aaaaaataaa gtagaaaaag aaattgtcca atgggtggat aagcctttgg agaataagga
     4141 agatgtatta aattgggtga agaatgggct aagatatttt cataatgtat cttatgtgga
     4201 gtctctaaaa gtcaaatttg agatgattac agaagctgat gatggatatg aaattttatt
     4261 gcagaatcat tcttattggt tgccaaaaaa cacaggagat ttaaaaagat agtttttttg
     4321 tgattatact gtgctatttc aagctaattt attgtacaag ttggtttgtt tatctaattc
     4381 ttctaaaaga actgtccata acaatatggg gcaggctgct gccaccgctg agcaataacc
     4441 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     4501 ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg
     4561 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     4621 gaaccgacga ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt
     4681 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     4741 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     4801 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     4861 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     4921 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     4981 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     5041 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     5101 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     5161 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     5221 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     5281 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     5341 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     5401 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     5461 atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta tcagggttat
     5521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca
     5581 ctcggtcgct acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta
     5641 cccacagatt ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc
     5701 cggtggcgtt tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt
     5761 ccggtgcatc tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc
     5821 gacaggactt aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt
     5881 ccgaccctgc cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc
     5941 tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg
     6001 ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact
     6061 tgagtccaac ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga
     6121 gttcgcagag gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc
     6181 ggctacactg gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta
     6241 agcagttccc caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt
     6301 tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6361 tactgaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
     6421 ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag
     6481 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     6541 taacttacat taattgcgtt gcgc
//
