LOCUS       sequence-399454-        4851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of ZooTsy (except YRec) proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT154: pHelper(ZooTsy)_Delta-YRec
FEATURES             Location/Qualifiers
     source          1..4851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      2755..2802
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2973..3764)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3765..3856)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3904..4642)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg agcgatacgc ccaatatcag acttggttgg cgcctccgcg
      121 tgcggatggc ggaaaaccga ataccgaccg ccacggaact caagcgacgg ctcgacgcga
      181 tcggctacga catcaccagc gcccaactct cccgaattgt ggacgagcga ccggcccagg
      241 tgaaaaccgc gctactggag gctttgctta aagtcctggg gggaacccta gacgatctca
      301 tgcccgcgga gggccgtgtt gagcagccga gttctgcgcc tcaaggtggt gaggaagttg
      361 cgccaagccc agcgccggcg gccaccccca cccgtaagcg caaaccggcg cgccccgcct
      421 cggaagcagt cgatccggac gatgccggtg gccccagggt acgccccttc tcggtcccac
      481 cgaagaagtg aaatagcgtt cctacatggc gacctctgct catacctgtt gcgatgaatg
      541 cagccgtccg atggccaagg cgcgtctcgt ccggaacggg aaggccttgt gcgacacctg
      601 cagccagcgg attccctacg ccccttgtgg caagtgcgga aagcagacgc gcatgctcaa
      661 tgaccaaggg gaaccgctct gcaaggtctg ccgtgccctc aaccgcacct gcgtgcgttg
      721 cggcaagcca gtgcgcaagg ctgggcgctt ggtcgatggc ggtgctgtct gcggcccctg
      781 cgccaagtat taccgtgaac cgcaggcctg cgatcaatgt ggggcaatgg agaaggttct
      841 taccggcgta cagacagaaa ctgggcggcg ccgtctctgc ggcaggtgtt tccggcgcca
      901 taaaggctac aagagctgca gcatctgccg gaaagacagg ctgcctgtcg gcgtgaccgc
      961 tgacggcaag cctatatgca aaacctgttt gagtgcggcc ggaaaacctt tcgtgtgccc
     1021 aacctgtggc caggaaggaa tacgccatag tagaaggcgt tgccagaagt gctacgttcg
     1081 cgatacggcg aaaagccgtg tcgaggaata tgctcccctt tttagtcatg catggacccg
     1141 ggaagcctgg ctgggatttg gtgcagagac cctggaggca atgaatccag aaaagctagt
     1201 gcatcgaatc aacagctact acctcttctt cgtccggctt gatctcgcct tcagcaaacc
     1261 tacagaaatc acgccggtcg cgctgttgaa aagcgtcggc ggcttggatg ggctccgtcg
     1321 atacaccgtg ccctatggct atctcctgaa gcaccgaatc atccccgaga caacgcgcag
     1381 tctcctgcag gacgaggctg aatccatcag gcaggaagcc atgatcgcca agctggatgg
     1441 caagtggtat gccccagtca tggcacgcta ccggaatcac ctgctgactg tccgcgcccg
     1501 atacgacagc cggggctgga aaggggaaga tacccggatg aaggcgcgct cgattacgct
     1561 caacctccgg gcggctcggc gtttatgcga aacgctggat gccataggtg tccagatgat
     1621 tcagcagacg acgccagaga tgctggacaa gttcttcatg gactatcctg gactggccgt
     1681 ctcaatccgt gccttcgtcc gatacctgaa ccggaaggaa aaactctttc agcgcctgcg
     1741 cctgaagaca gttgcccgga gtgtgccgga agggattttc ctgccccgcg aaaaatccac
     1801 tgcgctcatt caagcgtgga tgacgccgac tgatgagacg cttcggcagt ccctcatcgg
     1861 cctgttcatg ctgctctatg cccagacggt caagaactgt gttcggcttc gtctggatgc
     1921 actcatccaa cgggctgacg ggcgttatcg catagcattt ggccgcgcgg aaatccacct
     1981 ggatgctcgg atcagcgcga tactcgatcg ctatttgaac gtgcggcaaa cactggttgc
     2041 tctggaccat gccgacgaaa atccgtatct ctttccggga cggcagttag gcagtcacct
     2101 gagtgccgaa tcggtatccg gttggttgaa aaaggcgggc gtcagctccg atcagttgtt
     2161 tgcaacatca atctataacg cctatcaaaa tggcctgcga cttcccaagg tcttggtgcg
     2221 agcgtttggc atcaccgata cgaccgcgat caagtatctc aacatgctgg atccccgcct
     2281 ggtcatggag atcgaacgca aaccactggg cggcggggac gaaccctgat ggctggcgaa
     2341 cttaccgttc ggaaaaatcc atacgccagc ggtcagccgt ggtggctcta tatgctcgaa
     2401 tgcaagggcg gaggcatcta caccggcatc gcgctggatg tggatgcgcg gtttgagcaa
     2461 cacgtcgcag gcaagggcgc caaatacacg cgcatgaacc ggcccatccg ggtcgtcgcg
     2521 cgcatccgct acgacacgca ccgggaagcc gctcaggctg aagttgcgct gaaacgcctc
     2581 aagcccctgg aaaagctgcg gtgggcctat gccttggctg ggttagtcgg catggacaag
     2641 tcggcgtaac gcgtggggcg tctccggatc gttgaggggc aaaagcatcg cggcccccga
     2701 ggctgctgcc accgctgagc aataacctag gctgctgcca ccgctgagca ataactagca
     2761 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt
     2821 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     2881 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt
     2941 gcggcccctc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     3001 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     3061 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     3121 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     3181 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     3241 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     3301 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     3361 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     3421 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     3481 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     3541 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     3601 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     3661 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     3721 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatactctt cctttttcaa
     3781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3841 tagaaaaata aacaaatagc tagctcactc ggtcgctacg ctccgggcgt gagactgcgg
     3901 cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca ggggactaac
     3961 atgtgaggca aaacagcagg gccgcgccgg tggcgttttt ccataggctc cgccctcctg
     4021 ccagagttca cataaacaga cgcttttccg gtgcatctgt gggagccgtg aggctcaacc
     4081 atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggcg
     4141 ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgttccgc
     4201 ctttctccct tacgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc
     4261 ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg
     4321 ctgcgcctta tccggtaact gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc
     4381 actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg
     4441 ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg
     4501 ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa
     4561 accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg
     4621 atctcaagaa gatcctttga tcttttctac tgaaccgctc tagatttcag tgcaatttat
     4681 ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta
     4741 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     4801 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg c
//
