LOCUS       sequence-399460-        7406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AjTn6022 proteins (except TniQ)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT152: pHelper(AjTn6022)_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..7406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      5310..5357
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5528..6319)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(6320..6411)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6459..7197)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg aaaagaatca gtgatgaata ttccccagtt aggaaatccc
      121 aaactgtata tgggagtatt tcagtaaatt atgcttttag aggggaaaag acgatttggt
      181 gtgaatctac tcttgagcgt gattttttga ttaggcaaga attcaatagc aatgtcatag
      241 atatcgtaag ccagcctatt accatcccat atataacaga gttgggtaat gaatccactt
      301 atactcctga tttcctcgta cagttttcca gtgcaaattg tggtgatttt gaggaaattc
      361 cctgtccact gttaatagaa ataaaaccta gaaagaaact tatagaagat tgggataaat
      421 taaagccaaa atttaaggcg gctcatactt ttgctaaaga aaaaggttgg aaattcaaga
      481 ttataaatga atcaaggtta tatgatcagt attgggaaaa tattaatttt ttgaaaagat
      541 ttagacgttc tcatgtggat gtctatgatg aacaaacttt aattgagact ttagatagtc
      601 ttggtcaatc gactattaat caattaccgg cacatatttt taaggataaa actaatattc
      661 ttcgaggtat tcatcaagta tgggcattgg tcgcaaaacg aaaaatcatg tgtgatttat
      721 attgcccctt aacagcagag acggtgattt ggacaaatca agatgatgtg cttacaagga
      781 gatttttaaa tggttcagac tgatactgta agtattgacc gaagtcggat catactcaaa
      841 ccaaatgtaa ttgttaagta tgaagggcaa ccgtataaga ttgctaatgt tttgaatgcc
      901 aacgatatcg tgatttctag tttagattct gtgcgttgtt tacaagtaag tgcagattct
      961 ctacaagtat ttgaagctga aaataaccaa actaaagata taaataaagg tgattgggat
     1021 attacgcatg aagcgtggaa aatagcttta catcgttatg aaatcatcaa gccgttgatt
     1081 aagtattcaa caaccgaatt agtcgagcaa cgagcaagtg aatatggcat taaccgttcc
     1141 accttatgga aatggttaaa agattttaga gaaaataatt ctatcttagt gttagttcct
     1201 aaaaagagag gttggacgac caagaagtct aggttgtctc ctcaaattat gaatattatt
     1261 aaccaaggta ttcaagagga atatctcaat gccagaaagc ctagtattgc aaaaactata
     1321 gattttatta aagcagaatg ttctcgcctt aaactagaag caccccatga caattcaatt
     1381 cgtagaaaaa tagaagcatt aaacgattat atggtgatta aggctcgtca tggttctaaa
     1441 gcagcacaag ataagtatgc atctgctgct ggtgagtttc ctaatgctga ttatcccttg
     1501 gcatatgtac aggtagacca cactccacta gatattgaaa ttgttgatga tgaatttaga
     1561 gaggcgattg gacgaccatt tctaacatta gctattgata taaatactcg aatggtggtc
     1621 ggttattact tgtccttaga agcccccagt gcaacatctg ttgccatgtg tattgcttcc
     1681 agtattttgt caaaaaaacg gaagctaatt gaattggata tagatgctga ttggcaagtt
     1741 gaaggtctta tggattctat acatacagat aatggtcctg actttagaac taaccatatc
     1801 agtcgagctt gcttaaaata tgggattcat tgggagtttc gtccgattgg tggggcaagg
     1861 tttggtggac atatcgaacg tttgattggt gttgttaaca ttgaaatgca catcttggat
     1921 ggtacaacct tctcgaatat ccaagaacgt ggaacctata attcagcagg tcatgcctgt
     1981 atgacactaa aagaactaga atattatatt gtttattgga tcactaaagt ttatcaccaa
     2041 aagaaacact ccaaactcgg tatgtcacct ttacagaagt ggcaagatgg tgtttgggga
     2101 acaaaaacta gtgttggaac aggtttaaaa gagagagttt cggatgaaga tacattattt
     2161 attgatttcc ttcccgaatt tgaatcaaca attcaacgtg taggtgtaca gaaagacaat
     2221 ctattttatt ttgctgattg tttacgacaa tgggttaatt gtgttgatcc cgaagatgta
     2281 aataaaaaga gaaaaaagaa atttttgttt aaacgtgatc caagggatat ttctcttatt
     2341 tggttttatg aacctctgtc caatacatat tttaaagtgc ctactgctaa gcgagaaata
     2401 ccaccaatta gcttatttga atacaaacaa gtgcagaaat atcttcgtgg cgagcaacat
     2461 gataatcaaa atcaggatga gatttatcag gcaattcttc atttacgaga gcaattgaaa
     2521 caatcccgta gtctgactcg gaagcaacgg agagtaaatc aacgtaaaaa ggaaaatgca
     2581 aaagcaacac atcatttatc tgtaaaaaat gaacctgaaa aaccatctat tgtcgaaaac
     2641 agtgttcagc cgactaatga tttatggagt attccattga cggcatttga agatttaagg
     2701 ggataataat gcaaaaatat gaacatcttg cagatgatgt acttgaagtc atgtctctaa
     2761 gtgatagtga tcgtatcaat attttatttt ctgatcgttg gattggttat aaaaaagcgg
     2821 tcactatttt ggatacttta actgatctgt tgaatcgccc taggaagctt cgcccagaat
     2881 gtttattgat tgtgggtgat tctaatatgg gtaaaacgac cattatccac gaattcacaa
     2941 agcagcatta caccaaaaca gtgaatgaca tagatatgga gctgctgtct gtaaaaaaac
     3001 ctgttttaac catattagcc cctgctaaag cgaatgtaaa agaattctat atcaatatat
     3061 tgaatcattt ctttgtccca tttcgtccta ctgatcctga agcaaagtta cggaatcaag
     3121 ctgtccatct aatgcggaag tatgaaacaa aaatgttgat tattgatgaa atccataatt
     3181 gtttaacagg ttctaccaaa cttctttccg aagtgatgaa taccttaaag aatctaagta
     3241 atgatctaag tctgaatatc gttggcgtcg gtactcgtga agcagttacg attctacata
     3301 ccgatgctca atatgcaagc cgttttgatg tagcaatgtt gcctaaatgg gaactgaatc
     3361 aggattttct aagactatta ttaagttatg tacgattatt gcctttaaag aaacgttctg
     3421 atctagcatc tcaaagactt gccaccctta tatttgaaat ttcaggtggg aattttggtg
     3481 acttaaacag attgttgatt gagtgtgcta aagaggcaat cgtaaaaggt aatgaagaaa
     3541 ttaccgaaga aattgttaat aaatttaaat ggcttaaacc aaccgagggt atgaggaata
     3601 ttagaaatat aaatcttaac atggtttagg tattctactt ttcataggaa aatagccgtg
     3661 gttaaggcta ttgagaaagc tacagaaagg ggggaacagg tgactttagc tcaattttcc
     3721 cataaagaag gctgtagtgg ttgtgacagg gctgatatag ataaattcaa aaaaagatat
     3781 aagggcgata agtattgttc aacttgttat gcccgaattt ttaaaaaaag agcttgtcca
     3841 aaatgtggtg aatatgccag attgccaaaa aatgatgatc aagcaatttg taatgaatgc
     3901 atcaaaaaac agccttgtat tcgctgtaat caaactaaga agccgatagg gacactcacc
     3961 gaatatggtg tggtttgtaa tagctgttct gtttattttc gcccaattga gcgttgtgag
     4021 aggtgtgata caccatcaca aaaattgaca catatttcac gatttggtga tgatttaagg
     4081 gtatgtccca aatgctctac tagagattat gaaacatgtc cctcttgtca taaatatcgc
     4141 ttattagagc aagatgatac aggagctaag atttgtaaaa aatgtaggaa taacgccaaa
     4201 aagaaatgca agcaatgtga tgtcttgata ccagcaggct gtcctgatct atgtaacaac
     4261 tgttactggc ataagaatct atgggagaaa gctagacgaa atattaaagc attccaatcc
     4321 caacacttac aggctcaata tgagcaatat ttaatttggt tagaagatga ggttggtgca
     4381 aataaagcag cactgtatat caataaacat acccattttt ttatcaaaac tgaagaatta
     4441 tggcttgatg caattcctac agcagaacag ttattggctg ttttaagaac cagtggactt
     4501 agaaaatttg agttggttgc atcttggcta gatgaagccc atcacattaa ggttgcatta
     4561 gaggataaag acttttgctc tcaacaggat cagatagaaa aactgatttc aagccttcct
     4621 cacccctcta ctgcttatga tgtggttata tcttataaag atgagcttga tataaaaatg
     4681 aaagatggaa agaccagtat ccggtccata aaactggcaa taaagcctgc ggttgctctt
     4741 atgcactatg tttgtgcatc gggagctaca ttacctaatt taaaccatat aaaagcctat
     4801 cttatcgact tttctggtca ggctgctgca ctaacagggt tcattaattt tttgaataaa
     4861 aattttgata catctataga ctatttggct tttaaaaaat caaaaaattt caatgaaaaa
     4921 cgaaaaaata aagtagaaaa agaaattgtc caatgggtgg ataagccttt ggagaataag
     4981 gaagatgtat taaattgggt gaagaatggg ctaagatatt ttcataatgt atcttatgtg
     5041 gagtctctaa aagtcaaatt tgagatgatt acagaagctg atgatggata tgaaatttta
     5101 ttgcagaatc attcttattg gttgccaaaa aacacaggag atttaaaaag atagtttttt
     5161 tgtgattata ctgtgctatt tcaagctaat ttattgtaca agttggtttg tttatctaat
     5221 tcttctaaaa gaactgtcca taacaatatg gggcaggctg ctgccaccgc tgagcaataa
     5281 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     5341 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     5401 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
     5461 ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc ccctcggctt gaacgaattg
     5521 ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     5581 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     5641 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     5701 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     5761 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     5821 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     5881 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     5941 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     6001 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     6061 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     6121 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     6181 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     6241 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     6301 cgatcaccgc ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct
     6421 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
     6481 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
     6541 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
     6601 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
     6661 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
     6721 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
     6781 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     6841 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
     6901 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
     6961 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
     7021 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
     7081 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
     7141 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7201 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
     7261 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
     7321 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     7381 gctaacttac attaattgcg ttgcgc
//
