LOCUS       sequence-399522-       11812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent protein tagged beta-spectrin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Pacheco J, Cassidy AC, Zewe JP, Wills RC, Hammond GRV
  TITLE     PI(4,5)P2 diffuses freely in the plasma membrane even within 
            high-density effector protein complexes.
  JOURNAL   J Cell Biol. 2023 Feb 6;222(2):e202204099. doi: 
            10.1083/jcb.202204099. Epub 2022 Nov 23.
  PUBMED    36416724
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-02-6"; volume: "222(2):e202204099. doi"; pages: 
            " 10.1083/jcb.202204099. Epub 2022 Nov 23"
COMMENT     Sequence Label: EGFP-C3-beta-spectrin
FEATURES             Location/Qualifiers
     source          1..11812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             150..944
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             150..944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(204..223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     814..833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1132..1151)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1176..1223
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1552..2140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2041..2060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2315..2618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2619..2822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2773..2793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(2843..2845,2846..2848,2849..3559)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2843..2845,2846..2848,2849..3559)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2888..2909)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3149..3168)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3496..3517
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             6097..6105
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             10703..10729
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    10854..10975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10891..10910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10945..10964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10982..11437)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11119..11140)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11331..11350
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11464..11568
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(11570..11812,1..115)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11570..11761
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11593..11613)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(11778..11812,1..101)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
       61 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
      121 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
      181 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
      241 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
      301 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
      361 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
      421 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
      481 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
      541 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
      601 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
      661 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
      721 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
      781 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
      841 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
      901 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
      961 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     1021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     1081 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     1141 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     1201 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     1261 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     1321 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     1381 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     1441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2221 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     2281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     2341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2821 ctggtttagt gaaccgtcag atatggtgag caagggcgag gagctgttca ccggggtggt
     2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3541 catggacgag ctgtacaagc cgctagcgct accggactca gatctcgagc tcaagcttgc
     3601 cgccatgacg accacagtag ccacagacta tgacaacatt gagatccagc agcagtacag
     3661 tgatgtcaac aaccgctggg atgtcgacga ctgggacaat gagaacagct ctgcgcggct
     3721 ttttgagcgg tcccgcatca aggctctggc agatgagcgt gaagccgtgc agaagaagac
     3781 cttcaccaag tgggtcaatt cccaccttgc ccgtgtgtcc tgccggatca cagacctgta
     3841 cactgacctt cgagatggac ggatgctcat caagctgctg gaggtcctct ctggagagag
     3901 gctgcctaaa cccaccaagg gacgaatgcg catccactgc ttagagaatg tggacaaggc
     3961 ccttcagttc ctgaaggagc agagagtcca tcttgagaac atggggtccc atgacatcgt
     4021 ggatggaaac caccggctga cccttggcct catctggacc atcatcctgc gcttccagat
     4081 ccaggatatc agtgtggaaa ctgaagacaa caaagagaag aaatctgcca aggatgcatt
     4141 gctgttgtgg tgccagatga agacagctgg gtaccccaat gtcaacattc acaatttcac
     4201 cactagctgg agggacggca tggccttcaa tgcactgata cacaaacacc ggcctgacct
     4261 gatagatttt gacaaactaa agaaatctaa cgcacactac aacctgcaga atgcatttaa
     4321 tctggcagaa cagcacctcg gcctcactaa actgttggac cccgaagaca tcagcgtgga
     4381 ccatcctgat gagaagtcca taatcactta tgtggtgact tattaccact acttctctaa
     4441 gatgaaggcc ttagctgttg aaggaaaacg aattggaaag gtgcttgaca atgctattga
     4501 aacagaaaaa atgattgaaa agtatgaatc acttgcctct gaccttctgg aatggattga
     4561 acaaaccatc atcattctga acaatcgcaa atttgccaat tcactggtcg gggttcaaca
     4621 gcagcttcag gcattcaaca cttaccgcac tgtggagaaa ccacccaaat ttactgagaa
     4681 ggggaacttg gaagtgctgc tcttcaccat tcagagcaag atgagggcca acaaccagaa
     4741 ggtctacatg ccccgggagg ggaagctcat ctctgacatc aacaaggcct gggaaagact
     4801 ggaaaaagcg gaacacgaaa gagaactggc tttgcggaat gagctcataa gacaggagaa
     4861 actggaacag ctcgcccgca gatttgatcg caaggcagct atgagggaga cttggctgag
     4921 cgaaaaccag cgtctggtgt ctcaggacaa ctttgggttt gaccttcctg cagttgaggc
     4981 cgccacaaaa aagcacgagg ccattgagac agacattgcc gcatacgagg agcgtgtgca
     5041 ggctgtggta gccgtggcca gggagctcga ggccgagaat taccacgaca tcaagcgcat
     5101 cacagcgagg aaggacaatg tcatccggct ctgggaatac ctactggaac tgctcagggc
     5161 ccggagacag cggctcgaga tgaacctggg gctgcagaag atattccagg aaatgctcta
     5221 cattatggac tggatggatg aaatgaaggt gctagtattg tctcaagact atggcaaaca
     5281 cttacttggt gtggaagacc tgttacagaa gcacaccctg gttgaagcag acattggcat
     5341 ccaggcagag cgggtgagag gtgtcaatgc ctccgcccag aagttcgcaa cagacgggga
     5401 aggttacaag ccctgtgacc cccaggtgat ccgagaccgc gtggcccaca tggagttctg
     5461 ttatcaagag ctttgccagc tggcggctga gcgcagggcc cgtctggaag agtcccgccg
     5521 cctctggaag ttcttctggg agatggcaga agaggaaggc tggatacggg agaaggagaa
     5581 gatcctgtcc tcggacgatt acgggaaaga cctgaccagc gtcatgcgcc tgctcagcaa
     5641 gcaccgggcg ttcgaggacg agatgagcgg ccgcagtggc cactttgagc aggccatcaa
     5701 ggaaggcgaa gacatgatcg cggaggagca cttcgggtcg gagaagatcc gtgagaggat
     5761 catttacatc cgggagcagt gggccaacct agagcagctc tcggccattc ggaagaagcg
     5821 cctggaggag gcctccctgc tgcaccagtt ccaggcagat gctgatgaca ttgatgcctg
     5881 gatgctggac atcctcaaga ttgtctccag cagcgacgtg ggccacgatg agtattccac
     5941 acagtctctg gtcaagaaac acaaggacgt ggcggaagag atcgccaatt acaggcccac
     6001 ccttgacacg ctgcacgaac aagccagcgc cctcccccag gagcatgccg agtctccaga
     6061 cgtgaggggc aggctgtcgg gcatcgagga gcggtataag gaggtggcag agctgacgcg
     6121 gctgcggaag caggcactcc aggacactct ggccctgtac aagatgttca gcgaggctga
     6181 tgcctgtgag ctttggatcg acgagaagga gcagtggctc aacaacatgc agatcccaga
     6241 gaagctggag gatctggagg tcatccagca cagatttgag agcctagaac cagaaatgaa
     6301 caaccaggct tcccgggttg cagtggtgaa ccagattgca cgccagctga tgcacagcgg
     6361 ccacccaagt gagaaggaaa tcaaagccca gcaggacaaa ctcaacacaa ggtggagcca
     6421 gttcagagaa ctggttgaca ggaagaagga tgccctcctg tctgccctga gcatccagaa
     6481 ctaccacctc gagtgcaatg aaaccaaatc ctggattcgg gaaaagacca aggtcatcga
     6541 gtccacccag gacctgggca atgacctggc tggcgtcatg gccctgcagc gcaagctgac
     6601 cggcatggag cgggacttgg tggccattga ggcaaagctg agtgacctgc agaaggaggc
     6661 ggagaagctg gagtccgagc accccgacca ggcccaggcc atcctgtctc ggctggccga
     6721 gatcagcgac gtgtgggagg agatgaagac caccctgaaa aaccgagagg cctccctggg
     6781 agaggccagc aagctgcagc agttcctacg ggacttggac gacttccagt cctggctctc
     6841 taggacccag acagcgatcg cctcggagga catgccaaac accctgaccg aggctgagaa
     6901 gctgctcacg cagcacgaga acatcaagaa tgagatcgac aactacgagg aggactacca
     6961 gaagatgagg gacatgggcg agatggtcac ccaggggcag accgatgccc agtacatgtt
     7021 tctgcggcag cggctgcagg ccctggacac tggatggaac gagctccaca agatgtggga
     7081 gaacagacaa aatctcctat cccagtcaca tgcctaccag cagttcctca gagacacgaa
     7141 gcaagccgaa gcctttctta acaaccagga gtatgttctg gctcacactg aaatgcctac
     7201 caccttggaa ggagctgaag cagcaattaa aaagcaagag gacttcatga ccaccatgga
     7261 cgccaatgag gagaagatca atgctgtggt ggagactggc cggaggctgg tgagcgatgg
     7321 gaacatcaac tcagatcgca tccaggagaa ggtggactct attgatgaca gacataggaa
     7381 gaatcgtgag acagccagtg aacttttgat gaggttgaag gacaacaggg atctacagaa
     7441 attcctgcaa gattgtcaag agctgtctct ctggatcaat gagaagatgc tcacagccca
     7501 ggacatgtct tacgatgaag ccagaaatct gcacagtaaa tggttgaagc atcaagcatt
     7561 tatggcagaa cttgcatcca acaaagaatg gcttgacaaa atcgagaagg aaggaatgca
     7621 gctcatttca gaaaagcctg agacggaagc tgtggtgaag gagaaactca ctggtttaca
     7681 taaaatgtgg gaagtccttg aatccactac ccagacaaag gcccagcggc tctttgatgc
     7741 aaacaaggcc gaacttttca cccagagctg tgcagatcta gacaaatggc tgcacggcct
     7801 ggagagtcag attcagtctg atgactatgg caaagacctg accagtgtca atatcctgct
     7861 gaaaaagcaa cagatgctgg agaatcagat ggaagtgcgg aagaaggaga tcgaagagct
     7921 ccaaagccaa gcccaggccc tgagtcagga agggaagagc accgacgagg tagacagcaa
     7981 gcgcctcacc gtgcagacca agttcatgga gttgctggag cccttgaacg agaggaagca
     8041 taacctgctg gcctccaaag agatccatca gttcaacagg gatgtggagg acgagatctt
     8101 gtgggttgga gagaggatgc ctttggcaac ttccacggat catggccaca acctccagac
     8161 tgtgcagctg ttaataaaga aaaatcagac cctccagaaa gaaatccagg ggcaccagcc
     8221 tcgcattgac gacatctttg agaggagcca aaacatcgtc actgacagca gcagcctcag
     8281 cgctgaggcc atcagacaga ggcttgccga cctgaagcag ctgtggggtc tcctcattga
     8341 ggagacagag aaacgccaca ggcggctgga ggaggcgcac agggcccagc agtactactt
     8401 tgacgctgct gaggccgaag cctggatgag cgagcaggag ctgtacatga tgtcagagga
     8461 gaaggccaag gatgagcaga gtgctgtctc catgttgaag aagcaccaga tcttagaaca
     8521 agctgtggag gactatgcag agaccgtgca tcagctctcc aagaccagcc gggccctggt
     8581 ggccgacagc catcctgaaa gtgagcgcat tagcatgcgg cagtccaaag tggataaact
     8641 gtacgctggt ctgaaagacc ttgctgaaga gagaagaggc aagctggatg agagacacag
     8701 gttattccag ctcaaccggg aggtggacga cctggagcag tggatcgctg agagggaggt
     8761 ggtcgcaggg tcccatgaac tgggacagga ctatgagcat gtcacgatgt tacaagaacg
     8821 attccgggag tttgcccgag acaccgggaa cattgggcag gagcgcgtgg acacggtcaa
     8881 tcacctggca gatgagctca tcaactctgg acattcagat gccgccacca tcgctgaatg
     8941 gaaggatggc ctcaatgaag cctgggccga cctcctggag ctcattgaca caagaacaca
     9001 gattcttgcc gcttcctatg aactgcacaa gttttaccac gatgccaagg agatctttgg
     9061 gcgtatacag gacaaacaca agaaactccc tgaggagctt gggagagatc agaacacagt
     9121 ggagacctta cagagaatgc acactacatt tgagcatgac atccaggctc tgggcacaca
     9181 ggtgaggcag ctgcaggagg atgcagcccg cctccaggcg gcctatgcgg gtgacaaggc
     9241 cgacgatatc cagaagcgcg agaacgaggt cctggaagcc tggaagtccc tcctggacgc
     9301 ctgtgagagc cgcagggtgc ggctggtgga cacaggggac aagttccgct tcttcagcat
     9361 ggtgcgcgac ctcatgctct ggatggagga tgtcatccgg cagatcgagg cccaggagaa
     9421 gccaagggat gtatcatctg ttgaactctt aatgaataat catcaaggca tcaaagctga
     9481 aattgatgca cgtaatgaca gtttcacaac ctgcattgaa cttgggaaat ccctgttggc
     9541 gagaaaacac tatgcatctg aggagatcaa ggaaaaatta ctgcagttga cggaaaagac
     9601 gaaagaaatg atcgacaagt gggaagaccg atgggaatgg ttaagactga ttctggaggt
     9661 ccatcagttc tcaagagacg ccagtgtggc cgaggcctgg ctgcttggac aggagccgta
     9721 cctatccagc cgagagatag gccagagcgt ggacgaggtg gagaagctca tcaagcgcca
     9781 cgaggcattt gaaaagtctg cagcaacctg ggatgagagg ttctctgccc tggaaaggct
     9841 gactacattg gagttactgg aagtgcgcag acagcaagag gaagaggaga ggaagaggcg
     9901 gccaccttct cccgagccga gcacgaaggt ttcagaggaa gccgagtccc agcagcagtg
     9961 ggatacttca aaaggagaac aagtttccca aaacggtttg ccagctgaac agggatctcc
    10021 acggatggca gaaacggtgg acacaagcga aatggtcaac ggcgctacag aacaaaggac
    10081 gagctctaaa gagtccagcc ccatcccctc cccgacctct gatcgtaaag ccaagactgc
    10141 cctcccagcc cagagtgccg ccaccttacc agccagaacc caggagacac cttcggccca
    10201 gatggaaggc ttcctcaatc ggaaacacga gtgggaggcc cacaataaga aagcctcaag
    10261 tcgcagttgg cacaatgttt attgtgtcat aaataaccaa gaaatgggtt tctacaaaga
    10321 tgcaaagact gctgcttctg gaattcccta ccacagcgag gtccctgtga gtttgaaaga
    10381 agctgtctgc gaagtggccc ttgattacaa aaagaagaaa cacgtattca agctaagact
    10441 aaatgatggg aacgaatacc tcttccaagc caaagacgat gaggaaatga acacatggat
    10501 ccaggctatc tcttccgcca tctcctctga taaacacgag gtgtctgcca gcacccagag
    10561 cacgccagca tccagccgcg cgcagaccct ccccaccagc gtcgtcacca tcaccagcga
    10621 gtccagtccc ggcaagcggg aaaaggacaa agagaaagac aaagagaagc ggttcagcct
    10681 ttttggcaaa aagaaaccgc ggtacccata cgatgttcca gattacgctt aggcggccgc
    10741 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    10801 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    10861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
    10981 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    11041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    11101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    11161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    11221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    11281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    11341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    11401 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
    11461 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11521 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
    11581 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    11641 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    11701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    11761 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tc
//
