LOCUS       sequence-399573-        5695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMUT2 based vector for sensing oxygen in E. coli Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Vaaben TH, Vazquez-Uribe R, Sommer MOA
  TITLE     Characterization of Eight Bacterial Biosensors for Microbial 
            Diagnostic and Therapeutic Applications.
  JOURNAL   ACS Synth Biol. 2022 Dec 16;11(12):4184-4192. doi: 
            10.1021/acssynbio.2c00491. Epub 2022 Nov 30.
  PUBMED    36449712
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00491"; journalName: "ACS Synth Biol"; date: 
            "2022-12-16- 16"; volume: "11"; issue: "12"; pages: "4184-4192"
COMMENT     Sequence Label: pMUT2-pFF-41.5-mCherry
FEATURES             Location/Qualifiers
     source          1..5695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     79..98
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     321..338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(960..1754)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1755..1859)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2160..2182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2174..2191
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2175..2191
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2202..2221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2202..2220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2225..2236
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
       61 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      181 cgcctcccgc gcgcccttct tctgggtctt tgtcttttgc tcacatgttc tttccggttt
      241 tatcccccga ttctgtggat aaccgtatta ccgcctttga gtgagctgac accgctcgcc
      301 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccttatgt
      361 gacattttct ccttacgctc ctctatgccg ttctgcatcc tgtccggatg cgttatatcc
      421 cggtaagatt ttccgcttca aagcgtgtct gtatgctgtt ctggagttct tctgcgagtt
      481 cgtgcagttt ctcacacatg gcggcctgtt cgtcggcatt gagtgcgtcc agtttttcga
      541 gcagcgtcag gctctgactt tttatgaatc ccgccatgtt gagtacggct tgctgctgct
      601 tattcatctt ttcgttttct ccgttctgtc tgtcatctgc gttgtgtgat tatatcgcgt
      661 accacttttc gactgttttg ctgccgctat tctgccgctt ggctttttga cgggcatttc
      721 tgtcagacaa cactgtcact gccaaaaaac tgccgtgcct ttgtcggtaa ttcgagcttg
      781 ctgacaggac aggatgtgca attgttatac cgcgcataca tgcacgctat tacaattgcc
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
      961 tagaaaaatt catccagcag acgataaaac gcaatacgct ggctatccgg tgccgcaatg
     1021 ccatacagca ccagaaaacg atccgcccat tcgccgccca gttcttccgc aatatcacgg
     1081 gtggccagcg caatatcctg ataacgatcc gccacgccca gacggccgca atcaataaag
     1141 ccgctaaaac ggccattttc caccataatg ttcggcaggc acgcatcacc atgggtcacc
     1201 accagatctt cgccatccgg catgctcgct ttcagacgcg caaacagctc tgccggtgcc
     1261 aggccctgat gttcttcatc cagatcatcc tgatccacca ggcccgcttc catacgggta
     1321 cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg gacaggtcgc cgggtccagg
     1381 gtatgcagac gacgcatggc atccgccata atgctcactt tttctgccgg cgccagatgg
     1441 ctagacagca gatcctgacc cggcacttcg cccagcagca gccaatcacg gcccgcttcg
     1501 gtcaccacat ccagcaccgc cgcacacgga acaccggtgg tggccagcca gctcagacgc
     1561 gccgcttcat cctgcagctc gttcagcgca ccgctcagat cggttttcac aaacagcacc
     1621 ggacgaccct gcgcgctcag acgaaacacc gccgcatcag agcagccaat ggtctgctgc
     1681 gcccaatcat agccaaacag acgttccacc cacgctgccg ggctacccgc atgcaggcca
     1741 tcctgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     1861 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     1921 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     1981 ataaatcaaa agaatagacc gagatagggt tgagtggccg ctacagggcg ctcccattcg
     2041 ccattcaggc tgcgcaactg ttgggaaggg cgtttcggtg cgggcctctt cgctattacg
     2101 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2161 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgac gtaatacgac tcactatagg
     2221 gcgaattgaa ggaaggccgt caaggccacg tgtcttgtcc gcggtaccta cgaattcgag
     2281 ctcggtaccc ggggatcagg taaatttgat gtacatcaaa tggatccccc ctcactcctg
     2341 ccataattct gatattccag gaaagagagc catcctcagg agaattacaa tggtttccaa
     2401 gggcgaggag gataacatgg ctatcattaa agagttcatg cgcttcaaag ttcacatgga
     2461 gggttctgtt aacggtcacg agttcgagat cgaaggcgaa ggcgagggcc gtccgtatga
     2521 aggcacccag accgccaaac tgaaagtgac taaaggcggc ccgctgcctt ttgcgtggga
     2581 catcctgagc ccgcaattta tgtacggttc taaagcgtat gttaaacacc cagcggatat
     2641 cccggactat ctgaagctgt cttttccgga aggtttcaag tgggaacgcg taatgaattt
     2701 tgaagatggt ggtgtcgtga ccgtcactca ggactcctcc ctgcaggatg gcgagttcat
     2761 ctataaagtt aaactgcgtg gtactaattt tccatctgat ggcccggtga tgcagaaaaa
     2821 gacgatgggt tgggaggcgt ctagcgaacg catgtatccg gaagatggtg cgctgaaagg
     2881 cgaaattaaa cagcgcctga aactgaaaga tggcggccat tatgacgctg aagtgaaaac
     2941 cacgtacaaa gccaagaaac ctgtgcagct gcctggcgcg tacaatgtga atattaaact
     3001 ggacatcacc tctcataatg aagattatac gatcgtagag caatatgagc gcgcggaggg
     3061 tcgtcattct accggtggca tggatgagct gtacaaataa aaaggggcta ccggcgaacc
     3121 agcagcccct ttataaaggc gcttcagtag tcagaccagc atcagtcctg aaaaggcggg
     3181 cctgcgcccg cctccaggtt gctacttacc ggattcgtaa gccatgaaag ccgccacctc
     3241 cctgtgtccg tctctgtaac gaatctcgca cagcgatttt cgtgtcagat aagtgaatat
     3301 caacagtgtg agacacacga tcaacacaca ccagacaagg gaacttcgtg gtagtttcat
     3361 ggccttcttc tccttgcgca aagcgcggta agaggctatc ctgatgtgga ctagacatag
     3421 ggatgcctcg tggtggttaa tgaaaattaa cttactacgg ggctatcttc tttctgccac
     3481 acaacacggc aacaaaccac cttcacgtca tgaggcagaa agcctcaagc gccgggcaca
     3541 tcatagccca tatacctgca cgctgaccac actcactttc cctgaaaata atccgctcat
     3601 tcagaccgtt cacgggaaat ccgtgtgatt gttgccgcat cacgctgcct cccggagttt
     3661 gttcggcata agtcacaaga gacactcaat ggcaaaggaa gaccgtgccc tgttatccag
     3721 gcttatggca gattagcttc ccggagagaa aactgtcgaa aactgacggt atgaacaccg
     3781 taagctccca aagtgatcac cattcgcttt catgcatagc tatgcagcga gctgaaaacg
     3841 atcctgacgc atccttcctg tttttccggg gtaaaacatc tctttttgcg gtgtctgcgt
     3901 cagaatcgcg ttcagcgcgt ttcagtggtg cgtacaatta agggattatg gtaaatatat
     3961 gagctatgcg ataactttaa ctgtgaagcg atgaacccat tacaggcaaa gccaattact
     4021 cctgacagtg gtttagccag aagcagggct accaagacca atgcaataag taatatatcg
     4081 ttttgctatc gtgcatcgct cgcgctcagt tccattgtgc ttttttaagc tgtcgttttt
     4141 cttacggtat ataccggttt tttatggcgt ggtttcttaa cttgttcagc tactgatgga
     4201 cccattgtat ctaggtagtc aactagcttt gttagatcat aaatattgcg accaccatat
     4261 cggcgatcac tcttcgatgt tggtttcttg tccaagagat tagctttttc aagatcattg
     4321 atagctctct gaacagtccg tacagaaacc cccatacgta tggctagact ttccattgac
     4381 ggatgcggcc actcttgcaa actccaccag tgaacgatca ggttaagtag tgtgttaaag
     4441 gccactgaag ttagcttttt ctcgttttgt ataaaaaaca atacggtagg cactgctgtc
     4501 cagccaagag acaaaccgcc agctttccat ttattcttaa cggagtaagt cattgatttt
     4561 cctaagcccc aaaatattta aagtatatat tatatggtat attcatatga atagggtgac
     4621 actggcgcca ttattgtgca accaaaaaag actactctga aaacgaggaa aagatttttt
     4681 cctgcctgaa ttagatacgg agttagcgat atgaaaaccg aacaacgtca tgatcttgtt
     4741 aaagatattg aggtttttgg cgtatccttg tctctgttga tttccagagc gaatgagaag
     4801 tctgttacaa tgccatctgg tctaagtcgg gagcagagaa gagcatgggc agcggagcag
     4861 gcgcgcaaaa tccacaattg aatattgtct cattctctga gaccttcaac ctttattaca
     4921 catccagata ttctgcaaaa acactcgata aaatcgatga tttcattgag cattttgaaa
     4981 aatacaatct ctttggcgat cctttaaaag gatatccagc ttggactggc aaagtatcgc
     5041 catcgtggaa agtgcctgat cattacgaaa acaaagaagc tattgagaag tatgctagag
     5101 ctaacaaatt atggcatgct catttaggcg atccggtttt taaagatacg tttcatggga
     5161 aatacaaggt ttctgactgg gttattcatt tccagcggct gacaccgaac catataaagc
     5221 ttttagagct tggataccat gacccaatga agctacctca aaactttgaa tgatcgagcg
     5281 gcaggctaaa tgaaatcttg agattcattc agtctcgtcg taatctcact attgtaaaaa
     5341 cgaaaaaacc accctggcag gtggtttttc gaaggttagt taatcctggc agattctcta
     5401 accgtggtaa cagtcttgtg cgagacatgt caccaaatac tgtcctttca gtgtagcctc
     5461 agttaggccg ccacttcaag aactctcgtt acatctctcg cacatcctgc ttaccagtgg
     5521 ccgttgccag tggcgttaag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5641 cgacctacac cgaaccgaga taccaacagc gtgagctatg agaaagcgcc acgct
//
