LOCUS       sequence-399581-       19665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty pAGE2.0 plasmid with pBAD inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Cochrane RR, Shrestha A, Severo de Almeida MM, Agyare-Tabbi M, 
            Brumwell SL, Hamadache S, Meaney JS, Nucifora DP, Say HH, Sharma J, 
            Soltysiak MPM, Tong C, Van Belois K, Walker EJL, Lachance MA, Gloor 
            GB, Edgell DR, Shapiro RS, Karas BJ
  TITLE     Superior Conjugative Plasmids Delivered by Bacteria to Diverse 
            Fungi.
  JOURNAL   Biodes Res. 2022 Aug 19;2022:9802168. doi: 10.34133/2022/9802168. 
            eCollection 2022.
  PUBMED    37850145
REFERENCE   2  (bases 1 to 19665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2022-08-19"; volume: "2022:9802168. doi"; pages: " 
            10.34133/2022/9802168. eCollection 2022"
COMMENT     Sequence Label: pAGE2.0-i
FEATURES             Location/Qualifiers
     source          1..19665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        514..798
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     721..740
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             825..847
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             867..1544
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1589..1660
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1676..1703
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            1845..1954
                     /note="incP origin of transfer"
     CDS             1987..2358
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             3080..3649
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     rep_origin      8384..8998
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      8475..9006
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      9074..9293
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             9384..10139
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    10142..10392
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    10554..11057
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        11058..11244
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             11245..11907
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             12097..13272
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             13272..14243
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    14316..14789
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    15049..15447
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(15465..15498)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     15566..15585
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(15665..15940)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     15778..15800
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15792..15809
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15793..15809
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15816..15835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15816..15834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    15837..15893
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(15936..15952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15936..15952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15949..15971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15960..15976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15984..15990,15991..16008,16009..16014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16029..16050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(16270..16929)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     16844..16863
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(16930..17032)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(18072..18722)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             join(18828..19665,1..362)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc gcgacacggg
       61 gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga atgccggcgc
      121 tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg caaggctcac
      181 tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg gcgatctatg
      241 cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc ctctacttgc
      301 tctgcctgcc ggcgctgcgt cgcgggcttt ggagcggcgc agggcaacga gccgatcgct
      361 gatcgtggaa acgataggcc tatgccatgc gggtcaaggc gacttccggc aagctatacg
      421 cgccctagga gtgcggttgg aacgttggcc cagccagata ctcccgatca cgagcaggac
      481 gccgatgatt tgaagcgcac tcagcgtctg atcaagaaac caattgtcca tattgcatca
      541 gacattgccg tcactgcgtc ttttactggc acttctcgct aaccaaaccg gtaaccccgc
      601 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
      661 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
      721 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
      781 tctctactgt ttctccatac ccgttttttt gggctagaaa taattttgtt taactttaag
      841 aaggagatat acatatgcgg ggttctatgg cttcctccga agacgttatc aaagagttca
      901 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg
      961 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg
     1021 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt
     1081 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca
     1141 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct
     1201 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg
     1261 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc
     1321 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc
     1381 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg
     1441 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg
     1501 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct
     1561 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1621 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1681 accttcgggt gggcctttct gcgtttataa tcttccgctg cataaccctg cttcggggtc
     1741 attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa
     1801 agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa
     1861 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
     1921 ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc
     1981 aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat caaggtgtac
     2041 tgccttccag acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg
     2101 tcggcctacc tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag
     2161 cacgtccgcg agctggcccg catcaatggc gacctgggcc gcctgggcgg cctgctgaaa
     2221 ctctggctca ccgacgaccc gcgcacggcg cggttcggtg atgccacgat cctcgccctg
     2281 ctggcgaaga tcgaagagaa gcaggacgag cttggcaagg tcatgatggg cgtggtccgc
     2341 ccgagggcag agccatgact tttttagccg ctaaaacggc cggggggtgc gcgtgattgc
     2401 caagcacgtc cccatgcgct ccatcaagaa gaggcacttc gagctgtaag tacatcaccg
     2461 acgagcaagg caagacgatc atcgatacta gcttgattgg gatatctcgc tcgtgcttgt
     2521 cgcgtgctat gtcttttagg gtactttgaa cctacgttcg tacttgtata atatgatcat
     2581 cgtattatcg tttttcatcc gtccagcgca aaatgcatta gcagctagtc ctagcgtgcg
     2641 gagctacctg gacaggtgca tgacggatgc gtgtccttca gtgactttct aattaacagt
     2701 aacttcttta cttatgtttc agtttgtaag aagcgggatt cgctcgtcgg ttgacatctg
     2761 attggactgc gtcggcacat gaaaactaca ttgtgaaatc tgctaaaact ccgggtatct
     2821 ctgacacaaa acgattcggc ttcgcaattt caacattacg gtcaaggcta acgtatcttt
     2881 ctcggtcaac ttcagattat gccgattaaa ttgtcgtagc tttcaaggcg ttttgagtac
     2941 tgcggcagtt gttgaacctg caaggagaag atctcgacaa cagaataaag cgaaaaatgg
     3001 gtctcatgca ctaacactca ggcctccctc ataatctctg tttgagttta ccaacaacac
     3061 atatatacat ttcgacaaaa tgaccactct tgacgacacg gcttaccggt accgcaccag
     3121 tgtcccgggg gacgccgagg ccatcgaggc actggatggg tccttcacca ccgacaccgt
     3181 cttccgcgtc accgccaccg gggacggctt caccctgcgg gaggtgccgg tggacccgcc
     3241 cctgaccaag gtgttccccg acgacgaatc ggacgacgaa tcggacgacg gggaggacgg
     3301 cgacccggac tcccggacgt tcgtcgcgta cggggacgac ggcgacctgg cgggcttcgt
     3361 ggtcgtctcg tactccggct ggaaccgccg gctgaccgtc gaggacatcg aggtcgcccc
     3421 ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg ctcgcgacgg agttcgcccg
     3481 cgagcggggc gccgggcacc tctggctgga ggtcaccaac gtcaacgcac cggcgatcca
     3541 cgcgtaccgg cggatggggt tcaccctctg cggcctggac accgccctgt acgacggcac
     3601 cgcctcggac ggcgagcagg cgctctacat gagcatgccc tgcccctgat tttgttacat
     3661 ttactgactt caaggagtcg aggaatcgat actgccgtcg tttccaggat ccgaggtttc
     3721 ataaactctg ttaacgttat agaaacagac ttacctctcc tacgccattc acgtaatatt
     3781 cgcaatatgc tattcttcct ctgaagacca ggtttatgtg ctgcctgaaa ctatttcaat
     3841 aagtcagctg cacttgcaca gggtttcaca aggaaagcgt gtcttttttt ccaacgtagg
     3901 cgtcgctttc gtctgactct tactcttaca ttcacagcca atacttacaa ttagtaaaaa
     3961 acctgtgctc gagagtgaaa actccgtaac tataacggtc ctaaggtagc gaacactccg
     4021 ttcgtggcgt cgcacgatcg cgacatttga cgacgtgtgt tttgcctggg ctacgtatcg
     4081 ggaatgtttg cgaaaccccc gaaattgtac cgggatgtag gcacatcgct ggaaggtagc
     4141 caggggggtc cggcgctgat tacgaagtgc ggcgaccgac gaatttggag cacgcccgac
     4201 gctcaggggt ggatgccgct ggcgattccg cttgagtggg atcgctcggc acccgcgggt
     4261 cggcatatgc ccgtgccgat tgtgtcccca cggtgttggt caggcggccg gtatcacgac
     4321 accccccgcc catcgaagcc attcgctctc agcaacgcca tgagcatcgg gccaagcgag
     4381 aatttccctt gccgcgcctt ggcggtcaga tcgcgcagat agccgccagc agagttgata
     4441 tgccctgccc tctctagaat gcaggccacg gcgactccgg cgttttccgg tcccatgacc
     4501 tcgcaggcct cctgataggc cgaggggtaa acgccaagca tagatcgtac cgtgactgca
     4561 gcgatcatga ggtcgcgcca acttctcact gcaccgtcag gaccgtagtt catgatttcc
     4621 gggcaggctt tcagtaccat tcccagcgga aaggcccttt ttgccttgtt ggccggttca
     4681 atggcttggc cgtctaaccg ctcattccga gagcaacgtt caagttcaat aatagattcg
     4741 ggttttgatt cctgtttgtg ccgctcagtt tgatcgggat tgccgctcat ctcgtgtggt
     4801 tttatgtgct tttgcaactg gttagccacc tgttcgcgca gcgtttcgaa tgccactacc
     4861 aaaggggtga gatcagcgat atcgggcgtc ctcggtatcg cctggacgag gcttcggaaa
     4921 tcttcctgca tagacgtcca gtggcccggc acttctacga gcaccacgtc tatcaacttg
     4981 gcgatgtctc tgcggcagag cgtcagtcgc tcgcgtagcc gctgcagctc catccgatca
     5041 gcctcggctt cggcagcgag tcgctctatt tcgccggagc gggccagcag tggtgcgagc
     5101 gaaaagccga acgctgtgcc gatcgctcca tcgcgagtct tgcgtgcata gcgcttgccg
     5161 ttcggactgt ccttgcgagc gaccagccct gcatcgacga gggcggccag atgccttctg
     5221 agcgtagcct cggccatgcc atgagctctc agtgacagct gaacgttcga gggaaagacg
     5281 accagactgg tctccccgct tagcgtgttc tccggataga agctcaacag cgcgttcaga
     5341 acggctagtg tccgatcgct gatgccaaga cggcgtttgg cagcacaaag cgagcgaaac
     5401 aatttccact tgtcgaccga cttgccaggt tcgatgtcgc gggagatgaa ctggcctgcc
     5461 aacagggcaa gcgtcatcga ccgccgccca aagggcgtcg tcacacttcc aatctccatt
     5521 tccttcacct tcgataaggc aaaagaaagc agctcaccaa aaacggtgcc aaggactctt
     5581 gactgtgatt cgtggaaatg cgattctcga tcttgctaca aatctgagga aggcttccac
     5641 ggcttgttcg ttgggggcct ttttcttttg cgggttagtc tccttgtttc gaactctgct
     5701 tttgcatgaa ctggtcgtgc aggctttcga tctgacctgc aatgaaatcg acaaagcctg
     5761 acggcacaga cttgttgaat gtcagcttcg cagccgactt gccgcgttgg atctggccaa
     5821 gcacgaggcc atgagagatc agatcgctcg tggaagacgc tgcagccctc acatcaacct
     5881 tggtaagggc ttcaaaaacc gcagtgaagc gtagatcgct ggaagaggct tgaatctgct
     5941 cttgctccag aaccaactgc gccaccttcg cgggctcccg gtgagccgcc cagcgcactt
     6001 cgaactcgat ccatctacgc cgtccgattt caggagccgg accaatctgc atcaggatct
     6061 cgcgcggcaa tgctgccgct atccggatca tttcggagat atgcgacctg ccggtgctca
     6121 gggacttgca gatcacgtcg cggcgatagc cggcagcctc ctgcgattgc gcaaaaaggc
     6181 atcgctcgat gaaggacagg tccttgcggt tgctgttctc ttccccttgg agaacgatcg
     6241 catcttcgtc gctaagttac cgggcgattg ccttgaactt gatgccgagg agtttggcag
     6301 cggcaagccg gcgccggcca aatacgatct gaaacggcct tgcggcccct cgaaccgggc
     6361 ggaccaagcc aggagtactc tgtccatgct cgcgcatcga ctcgagcaaa tccgcaatac
     6421 cggcttcgct gtagccggaa tcaaagcgat cccggatggc cgattccatg atctcgtctg
     6481 gatcgatctc gacgatctgg ccaccatcct tgagaaggcg ttcggcaagt tcgctgcgtt
     6541 cctgcatctg acggacgccg gctgcaactt tgagaagatg tggcgatgtc gaccgctgca
     6601 tcccgccctt ctctgcagaa ttttccagct gtccgaggac attggcaaaa aggcccttgg
     6661 aatccttgcg gctcattctc tcccccatgc cttccagaag aggccctcga gttcagcatt
     6721 ggcagcattg atcgactcga tggcgcggtc gtaggttttc ggcgccgaaa atcgggacct
     6781 ctgaacctcg taaaggctct gcttccacgt cagagcatcg gccaccgcgg tcgacttgat
     6841 gacggagttg aggagcagat cctcgccgaa cacctgtctc atcagcgcgg acatattcgc
     6901 ttgcggatga tcgttcggct cgaactttgt gatcaggaac ttggcgaagt cccattgagc
     6961 ggcggtgccc acctcattca ggatctgcat gtacgacccg gcaagctcca aaaactttgc
     7021 ggtcgagtcc acatccagca tatgtgccgg aaccgggatg atgactccag tcgcggcggt
     7081 cagcgacgaa agtgtcagga agttcagcga cggagccgag tccataagga cgaagtcgta
     7141 gtcatcggcg acctgctcca gagcttgaga aacgcgaagc aagaaaccgg ttcctgctga
     7201 ccgccgcatc tcgagggcga cggccgtctc gaactcggtc agttcgaggc cggcacagat
     7261 gacgtcgaaa ccaacgatgt gggtcttgtg gatgatggat ttcgtcggga cgggatcgtc
     7321 gaaacgaatg gctgagaaaa gtgtatcgcc gccgcggtaa tcgaattcgg gcaggctacc
     7381 atggagcgcg gtgagagatg cttgcgggtc gagatcgacc gccagcacgc gatagccctt
     7441 gagtgccaga tagtgaccga gatggattgt ggtagaggtc ttgctggagc cgcccttgaa
     7501 gttggtgacc gcaacgatct ggcattgctc attgcctgtc cggcgggggt tcatccattt
     7561 cttccggccg tttgccgcaa gagccatacg tagctcaaga acctgctcga gcgtgtagaa
     7621 gcgccggccg ttggcgattt cgggctcagg gccctgttcc ttggcggcaa cctggcggat
     7681 ataggcctcg gttacgccca gcagggtggc aacttcggta gaggtgaact tgcgcattgt
     7741 gcgacgcgcg ttcgggggga actgcgccat gctcaagttg ttcagtgcca tttcgagctt
     7801 actggaaaaa ttctccatga aagcgaggct gcttaggtgt cgcgtcatcg aaagaatccc
     7861 tgtgatcgtt tccgtcactt aagcagtgaa gtggttaacc gcttgctaat cgccacgtaa
     7921 ctactcgaaa acacgcaaat tcgaaaaatt tccgcaacta agacgtactc cgattcggct
     7981 atggccatca atgcgcgctg tggatgacgc acagagccgg ggggaaggcg ccagtgcatg
     8041 ccggttctcg accctccccg cctctttttt ggacgaagat gtgcgcagac gtcgctgggc
     8101 ggagacgcaa tagtggttca ctgcggaaat ggttggccgt tgctttcaat ggagatctgc
     8161 cgaactcttc gcagagttcc ccgcggggaa ctctcgaagc gacgacgctg gcgcgcagta
     8221 ccgactgctc gccaaggcga gacatatgtg ccgtattggg aacagctcgg catctcggtg
     8281 ctcgcctgcg ctccggcctg cctcaatctc gttggccatg accgagcaac gactgcaccg
     8341 gatggttcag ttacgctagg gataacaggg taatatagtc gagctggttg ccctcgccgc
     8401 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
     8461 gagacaccgc ggccggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg
     8521 acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag
     8581 atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg
     8641 aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg
     8701 ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga
     8761 cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt
     8821 ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac
     8881 ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg
     8941 ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg
     9001 tcgagtgagc gaggaagcac cagggaacag cacttatata ttctgcttac acacgatgcc
     9061 tgaaaaaact tcccttgggg ttatccactt atccacgggg atatttttat aattattttt
     9121 tttatagttt ttagatcttc ttttttagag cgccttgtag gcctttatcc atgctggttc
     9181 tagagaaggt gttgtgacaa attgcccttt cagtgtgaca aatcaccctc aaatgacagt
     9241 cctgtctgtg acaaattgcc cttaaccctg tgacaaattg ccctcagaag aagctgtttt
     9301 ttcacaaagt tatccctgct tattgactct tttttattta gtgtgacaat ctaaaaactt
     9361 gtcacacttc acatggatct gtcatggcgg aaacagcggt tatcaatcac aagaaacgta
     9421 aaaatagccc gcgaatcgtc cagtcaaacg acctcactga ggcggcatat agtctctccc
     9481 gggatcaaaa acgtatgctg tatctgttcg ttgaccagat cagaaaatct gatggcaccc
     9541 tacaggaaca tgacggtatc tgcgagatcc atgttgctaa atatgctgaa atattcggat
     9601 tgacctctgc ggaagccagt aaggatatac ggcaggcatt gaagagtttc gcggggaagg
     9661 aagtggtttt ttatcgccct gaagaggatg ccggcgatga aaaaggctat gaatcttttc
     9721 cttggtttat caaacgtgcg cacagtccat ccagagggct ttacagtgta catatcaacc
     9781 catatctcat tcccttcttt atcgggttac agaaccggtt tacgcagttt cggcttagtg
     9841 aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc
     9901 gtaagccgga tggctcaggc atcgtctctc tgaaaatcga ctggatcata gagcgttacc
     9961 agctgcctca aagttaccag cgtatgcctg acttccgccg ccgcttcctg caggtctgtg
    10021 ttaatgagat caacagcaga actccaatgc gcctctcata cattgagaaa aagaaaggcc
    10081 gccagacgac tcatatcgta ttttccttcc gcgatatcac ttccatgacg acaggatagt
    10141 ctgagggtta tctgtcacag atttgagggt ggttcgtcac atttgttctg acctactgag
    10201 ggtaatttgt cacagttttg ctgtttcctt cagcctgcat ggattttctc atactttttg
    10261 aactgtaatt tttaaggaag ccaaatttga gggcagtttg tcacagttga tttccttctc
    10321 tttcccttcg tcatgtgacc tgatatcggg ggttagttcg tcatcattga tgagggttga
    10381 ttatcacagt ttattactct gaattggcta tccgcgtgtg tacctctacc tggagttttt
    10441 cccacggtgg atatttcttc ttgcgctgag cgtaagagct atctgacaga acagttcttc
    10501 tttgcttcct cgccagttcg ctcgctatgc tcggttacac ggctgcggcg agcatcacgt
    10561 gctataaaaa taattataat ttaaattttt taatataaat atataaatta aaaatagaaa
    10621 gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag
    10681 ggggatcgcc aacaaatact accttttacc ttgctcttcc tgctctcagg tattaatgcc
    10741 gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt
    10801 tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat
    10861 atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt
    10921 ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata
    10981 aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa
    11041 gttacaggca agcgatccta gtacactcta tattttttta tgcctcggta atgattttca
    11101 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
    11161 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
    11221 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
    11281 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
    11341 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
    11401 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
    11461 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
    11521 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
    11581 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
    11641 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
    11701 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
    11761 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
    11821 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
    11881 ccctccacca aaggtgttct tatgtagttt tacacaggag tctggacttg acgctagtga
    11941 taataagtga ctgaggtatg tgctcttctt atctcctttt gtagtgttgc tcttatttta
    12001 aacaactttg cggttttttg atgactttgc gattttgttg ttgctttgca gtaaattgca
    12061 agatttaata aaaaaacgca aagcaatgat taaaggatgt tcagaatgaa actcatggaa
    12121 acacttaacc agtgcataaa cgctggtcat gaaatgacga aggctatcgc cattgcacag
    12181 tttaatgatg acagcccgga agcgaggaaa ataacccggc gctggagaat aggtgaagca
    12241 gcggatttag ttggggtttc ttctcaggct atcagagatg ccgagaaagc agggcgacta
    12301 ccgcacccgg atatggaaat tcgaggacgg gttgagcaac gtgttggtta tacaattgaa
    12361 caaattaatc atatgcgtga tgtgtttggt acgcgattgc gacgtgctga agacgtattt
    12421 ccaccggtga tcggggttgc tgcccataaa ggtggcgttt acaaaacctc agtttctgtt
    12481 catcttgctc aggatctggc tctgaagggg ctacgtgttt tgctcgtgga aggtaacgac
    12541 ccccagggaa cagcctcaat gtatcacgga tgggtaccag atcttcatat tcatgcagaa
    12601 gacactctcc tgcctttcta tcttggggaa aaggacgatg tcacttatgc aataaagccc
    12661 acttgctggc cggggcttga cattattcct tcctgtctgg ctctgcaccg tattgaaact
    12721 gagttaatgg gcaaatttga tgaaggtaaa ctgcccaccg atccacacct gatgctccga
    12781 ctggccattg aaactgttgc tcatgactat gatgtcatag ttattgacag cgcgcctaac
    12841 ctgggtatcg gcacgattaa tgtcgtatgt gctgctgatg tgctgattgt tcccacgcct
    12901 gctgagttgt ttgactacac ctccgcactg cagtttttcg atatgcttcg tgatctgctc
    12961 aagaacgttg atcttaaagg gttcgagcct gatgtacgta ttttgcttac caaatacagc
    13021 aatagcaatg gctctcagtc cccgtggatg gaggagcaaa ttcgggatgc ctggggaagc
    13081 atggttctaa aaaatgttgt acgtgaaacg gatgaagttg gtaaaggtca gatccggatg
    13141 agaactgttt ttgaacaggc cattgatcaa cgctcttcaa ctggtgcctg gagaaatgct
    13201 ctttctattt gggaacctgt ctgcaatgaa attttcgatc gtctgattaa accacgctgg
    13261 gagattagat aatgaagcgt gcgcctgtta ttccaaaaca tacgctcaat actcaaccgg
    13321 ttgaagatac ttcgttatcg acaccagctg ccccgatggt ggattcgtta attgcgcgcg
    13381 taggagtaat ggctcgcggt aatgccatta ctttgcctgt atgtggtcgg gatgtgaagt
    13441 ttactcttga agtgctccgg ggtgatagtg ttgagaagac ctctcgggta tggtcaggta
    13501 atgaacgtga ccaggagctg cttactgagg acgcactgga tgatctcatc ccttcttttc
    13561 tactgactgg tcaacagaca ccggcgttcg gtcgaagagt atctggtgtc atagaaattg
    13621 ccgatgggag tcgccgtcgt aaagctgctg cacttaccga aagtgattat cgtgttctgg
    13681 ttggcgagct ggatgatgag cagatggctg cattatccag attgggtaac gattatcgcc
    13741 caacaagtgc ttatgaacgt ggtcagcgtt atgcaagccg attgcagaat gaatttgctg
    13801 gaaatatttc tgcgctggct gatgcggaaa atatttcacg taagattatt acccgctgta
    13861 tcaacaccgc caaattgcct aaatcagttg ttgctctttt ttctcacccc ggtgaactat
    13921 ctgcccggtc aggtgatgca cttcaaaaag cctttacaga taaagaggaa ttacttaagc
    13981 agcaggcatc taaccttcat gagcagaaaa aagctggggt gatatttgaa gctgaagaag
    14041 ttatcactct tttaacttct gtgcttaaaa cgtcatctgc atcaagaact agtttaagct
    14101 cacgacatca gtttgctcct ggagcgacag tattgtataa gggcgataaa atggtgctta
    14161 acctggacag gtctcgtgtt ccaactgagt gtatagagaa aattgaggcc attcttaagg
    14221 aacttgaaaa gccagcaccc tgatgcgacc tcgttttagt ctacgtttat ctgtctttac
    14281 ttaatgtcct ttgttacagg ccagaaagca taactggcct gaatattctc tctgggccca
    14341 ctgttccact tgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg
    14401 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
    14461 ctgggaccac ggtcccactc gtatcgtcgg tctgataatc agactgggac cacggtccca
    14521 ctcgtatcgt cggtctgatt attagtctgg gaccatggtc ccactcgtat cgtcggtctg
    14581 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctggaacc
    14641 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc
    14701 gtcggtctga ttattagtct gggaccacga tcccactcgt gttgtcggtc tgattatcgg
    14761 tctgggacca cggtcccact tgtattgtcg atcagactat cagcgtgaga ctacgattcc
    14821 atcaatgcct gtcaagggca agtattgaca tgtcgtcgta acctgtagaa cggagtaacc
    14881 tcggtgtgcg gttgtatgcc tgctgtggat tgctgctgtg tcctgcttat ccacaacatt
    14941 ttgcgcacgg ttatgtggac aaaatacctg gttacccagg ccgtgccggc acgttaaccg
    15001 ggctgcatcc gatgcaagtg tgtcgctgtc gacgagctcg cgagctcgga catgaggttg
    15061 ccccgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt aagttgatgc
    15121 agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt gatggcctcc
    15181 acgcacgttg tgatatgtag atgataatca ttatcacttt acgggtcctt tccggtgatc
    15241 cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat tttccggttt
    15301 aaggcgtttc cgttcttctt cgtcataact taatgttttt atttaaaata ccctctgaaa
    15361 agaaaggaaa cgacaggtgc tgaaagcgag ctttttggcc tctgtcgttt cctttctctg
    15421 tttttgtccg tggaatgaac aatggaagtc cgagctcatc gctaataact tcgtatagca
    15481 tacattatac gaagttatat tcgatgcggc cgcaaggggt tcgcgtcagc gggtgttggc
    15541 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    15601 atgcggtgtg aaataccaca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    15661 ccattcagct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    15721 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    15781 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
    15841 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttgagtatt
    15901 ctatagtctc acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    15961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    16021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    16081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgaacc ccttgcggcc
    16141 gcccgggccg tcgaccaatt ctcatgtttg acagcttatc atcgaatttc tgccattcat
    16201 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
    16261 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
    16321 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
    16381 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
    16441 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
    16501 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
    16561 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
    16621 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
    16681 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
    16741 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
    16801 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
    16861 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
    16921 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
    16981 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
    17041 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
    17101 ttctgcgaag tgatcttccg tcacaggtat ttattcgcga taagctcatg gagcggcgta
    17161 accgtcgcac aggaaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
    17221 ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg ttctctgttc
    17281 cggtcacacc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
    17341 cgctgaaagt tctgcaaagc ctgatgggac ataagtccat cagttcaacg gaagtctaca
    17401 cgaaggtttt tgcgctggat gtggctgccc ggcaccgggt gcagtttgcg atgccggagt
    17461 ctgatgcggt tgcgatgctg aaacaattat cctgagaata aatgccttgg cctttatatg
    17521 gaaatgtgga actgagtgga tatgctgttt ttgtctgtta aacagagaag ctggctgtta
    17581 tccactgaga agcgaacgaa acagtcggga aaatctccca ttatcgtaga gatccgcatt
    17641 attaatctca ggagcctgtg tagcgtttat aggaagtagt gttctgtcat gatgcctgca
    17701 agcggtaacg aaaacgattt gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt
    17761 tacgttgaag tggagcggat tatgtcagca atggacagaa caacctaatg aacacagaac
    17821 catgatgtgg tctgtccttt tacagccagt agtgctcgcc gcagtcgagc gacagggcga
    17881 agcccgttta aacgagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg
    17941 gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg
    18001 gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc
    18061 agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc
    18121 cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg
    18181 gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg
    18241 gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca
    18301 gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg
    18361 cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg
    18421 gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc
    18481 ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc
    18541 gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa
    18601 agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg
    18661 ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc
    18721 atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga
    18781 atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca
    18841 tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta
    18901 tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact
    18961 atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg
    19021 cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg
    19081 tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg
    19141 tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg
    19201 atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg
    19261 tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg
    19321 ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc
    19381 acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt
    19441 gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg
    19501 tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg
    19561 cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa
    19621 tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcac
//
