LOCUS       sequence-399626-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the 
            N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Cheng Y, Zhang Y, Li G, Fang H, Sretenovic S, Fan A, Li J, Xu J, Que
            Q, Qi Y
  TITLE     CRISPR-Cas12a base editors confer efficient multiplexed genome 
            editing in rice.
  JOURNAL   Plant Commun. 2023 Apr 13:100601. doi: 10.1016/j.xplc.2023.100601.
  PUBMED    37060177
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2023 Apr 13:100601. doi: 10.1016/j.xplc.2023.100601."
COMMENT     Sequence Label: dpYPQ230-D156R-CBE4
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             804..824
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctccgaattc gcccttcatg gaggcctctc
      121 cggcttcagg tcctcgccat ctcatggacc cccatatctt tacctccaat ttcaataacg
      181 gcattggcag gcacaagaca tacctctgtt atgaggtcga gcggctcgat aacggaacct
      241 cagtgaagat ggaccaacac aggggtttcc ttcataacca agcgaaaaat ctcctttgcg
      301 gtttctatgg gcggcacgcg gagctgagat tcttggattt ggtcccgagc ctccagctgg
      361 atcccgcgca aatataccgc gtcacttggt tcatatcatg gagcccgtgc tttagctggg
      421 ggtgtgccgg tgaggttcgc gcgttcctcc aggaaaacac ccatgttcgc ctccggattt
      481 tcgcagcgag gattttcgac tatgatccac tttacaaaga ggccctccaa atgcttagag
      541 atgctggggc tcaagtttca attatgacat acgacgagtt taagcattgt tgggacactt
      601 ttgtcgatca tcagggttgt cccttccagc cgtgggatgg tcttgatgag cattcgcaag
      661 ctttgagtgg caggcttcgc gcgatacttc agaaccaggg taacgggggc gggggctcag
      721 gcgggggcgg gagcggcggc gggggctctg ggggcggcgg cagcggcggg ggcggcagcg
      781 ggggcggcgg gtcgaccatg gctcctaaga agaagcggaa ggttggtatt cacggggtgc
      841 ctgcggcttc aaagctcgag aaattcacca actgttattc g
//
