LOCUS       sequence-399636-        9696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 
            with CMV for mcherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mch...
FEATURES             Location/Qualifiers
     source          1..9696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2196..2444
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2196..2216
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2367..2386
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4370..4673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4674..4877
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4828..4848
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4916..4925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4922..5632
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5067..5085)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5335..5354
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5665..6253
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5718..5738)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6136..6147)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6460..7093
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7221..7237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7221..7237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7269..7275,7276..7293,7294..7299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7314..7335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7452..7469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7623..8211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7703..7722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8382..9173,9174..9242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9005..9024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9243..9347)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9395..9529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9432..9451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9486..9505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt
     2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccgtccga gtaatttacg
     2461 ttttgatacg gttgcggaac cgaccagaat catgcaagtg cgtaagatag tcgcgggccg
     2521 ggggaagagg aagaagcagc cctgaggatg gcggcggcgg cggccagcac cggccggtgg
     2581 gcgggagccc cggcgggccg cgcctggtga tcggctcctt accagctcac ctctcaccgc
     2641 gcatgtttgg agactatatc atcatgtaca tagaaaaatc caaaggattc tgcaaaaaag
     2701 ctactagaac cactgacttc atcaagatgc agtctccctc tgatgccgag cggaggctgg
     2761 actgtacagc caccatctcg gctcactgca acatccctgc ctgattctcc tgcctcagcc
     2821 tgccgagtgc ctgggattgc aggctcgcgc cgccacgcct gactggtttt tgtatttttt
     2881 ggtggagaag gggtttcgcc atgttggctg ggctggtctc cagctcctga ccgcaagtga
     2941 tctgcccgcc tcggcctccc gaggtgccag gattgcagac ggagtctcgc tcactcagtg
     3001 ctcaatgttg cccaggctgg agtgcagtgg cgtgatctca gctcgctaca acctccacct
     3061 cccagccgcc tgccttggcc tcccaaagtg ctgagattgc agcctctgcc cggccgccac
     3121 cccgtctggg aagtgaggag cgtctctgcc tggccgccca tcgtctggga tgtgaggagc
     3181 ccctctgccc ggccgcccag tctgagatgt gaagagcgcc tctgcccggc cgcgaccccg
     3241 tctgggaact gaggagtgtc tctgcctggc cacccatcgt ctgggatgtg aggagcccct
     3301 ctgcccggcc gcccagtctg ggaaatgagg agcgcctctt cctggccacc atcccgtctg
     3361 ggaagtgagg agcgtctctg cccggccgcc catcgtctga gatgtgggga gcgcctctgc
     3421 ccggccccaa ccccgtctgg gaactgagga gtgtctctgc ccgaccgcca ccccgtctgg
     3481 gaggtgagga acgtttctgc ccagccgccc gtctgagaag tgagcccctc cgcctggcag
     3541 ccgccccatc tgggaagtga ggagcccctc cgcccggcag ccgccccgtc cgggaggtgg
     3601 ggggcagccc ccacccggcc agccaccccg cacgggaggg aggtggggcc agcccctgcc
     3661 tggccagccg ccccattcag gaggtggggg gcagcccctg cccggcagcc atcccgtccg
     3721 ggaggtgggg ggcgcctctg cccggctgcc ccatctggga agtgaggagc ccctctgccc
     3781 ggcagccacc ccgtctggga ggtgtgccca acagctcatt gagaacgggc catgatgacg
     3841 atggcggttt tgtcgaatag aaagggggga aatgtgggga aaagaaagag aaatcagatt
     3901 gttgcggcgt ctgtgtagaa agaagtagat ataggagact ccattttgtt ctgtactaag
     3961 aaaaattctt ctgccttggg atgctgttaa tctataacct tacccccaac cccgtgctct
     4021 ctgaaacatg tgctgtgtcc actcagggtt aaatggatta agggcggtgc aagatgtgct
     4081 ttgttaaaca gatgcttgaa ggcagcatgc tcgttaagag tcatcaccac tccctaatct
     4141 caagtaccca gggacacaaa cactgcggaa ggctgcaggg tcctctgcct aggaaaacca
     4201 gagacccttg ttcacatgtt tatctgctga ccttccctcc actattgtcc tatgaccctg
     4261 ccaaatcccc ctctccaaaa aacacccaag aatgatcaaa aaagaattct agttattaat
     4321 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     4381 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     4441 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     4501 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     4561 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     4621 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     4681 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     4741 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     4801 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     4861 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     4921 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4981 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     5041 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     5101 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     5161 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     5221 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     5281 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     5341 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     5401 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     5461 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     5521 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5581 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt agctcgagtc
     5641 tagagggccc gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     5701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     6061 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     6121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     6181 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6241 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     6301 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     6361 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     6421 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     6481 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6541 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     6601 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     6661 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     6721 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     6781 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     6841 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     6901 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7081 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7141 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9361 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
     9421 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9481 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     9541 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     9601 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     9661 ttgtatttgt taaatatgta ctacaaactt agtagt
//
