LOCUS       sequence-399638-       11235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express SVA-lncRNA AK057321 with ires for 
            mcherry and puro expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
FEATURES             Location/Qualifiers
     source          1..11235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..235
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1810..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2130..3312
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2361..3303
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3260..3280
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5137..5710
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5160..5712
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5304..5321)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5531..5550
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5741..5750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5747..6454
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5892..5910)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6160..6179
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6473..7072
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6473..6492)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6969..6989
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7087..7675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7140..7160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7558..7569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7762..7995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8067..8188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8104..8123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8158..8177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8228..8363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8383..8402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8384..8402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8412..8429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8412..8428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8421..8443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8570..9025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8657..8676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8867..8888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9051..9155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9156..9224,9225..10016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9374..9393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10187..10775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10676..10695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10929..10946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11063..11084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11099..11104,11105..11122,11123..11129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11137..11153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11142..11164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11161..11177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11161..11177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11196..11216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11198..11216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttt tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctctagaag cagccctgag gatggcggcg gcggcggcca
     3361 gcaccggccg gtgggcggga gccccggcgg gccgcgcctg gtgatcggct ccttaccagc
     3421 tcacctctca ccgcgcatgt ttggagacta tatcatcatg tacatagaaa aatccaaagg
     3481 attctgcaaa aaagctacta gaaccactga cttcatcaag atgcagtctc cctctgatgc
     3541 cgagcggagg ctggactgta cagccaccat ctcggctcac tgcaacatcc ctgcctgatt
     3601 ctcctgcctc agcctgccga gtgcctggga ttgcaggctc gcgccgccac gcctgactgg
     3661 tttttgtatt ttttggtgga gaaggggttt cgccatgttg gctgggctgg tctccagctc
     3721 ctgaccgcaa gtgatctgcc cgcctcggcc tcccgaggtg ccaggattgc agacggagtc
     3781 tcgctcactc agtgctcaat gttgcccagg ctggagtgca gtggcgtgat ctcagctcgc
     3841 tacaacctcc acctcccagc cgcctgcctt ggcctcccaa agtgctgaga ttgcagcctc
     3901 tgcccggccg ccaccccgtc tgggaagtga ggagcgtctc tgcctggccg cccatcgtct
     3961 gggatgtgag gagcccctct gcccggccgc ccagtctgag atgtgaagag cgcctctgcc
     4021 cggccgcgac cccgtctggg aactgaggag tgtctctgcc tggccaccca tcgtctggga
     4081 tgtgaggagc ccctctgccc ggccgcccag tctgggaaat gaggagcgcc tcttcctggc
     4141 caccatcccg tctgggaagt gaggagcgtc tctgcccggc cgcccatcgt ctgagatgtg
     4201 gggagcgcct ctgcccggcc ccaaccccgt ctgggaactg aggagtgtct ctgcccgacc
     4261 gccaccccgt ctgggaggtg aggaacgttt ctgcccagcc gcccgtctga gaagtgagcc
     4321 cctccgcctg gcagccgccc catctgggaa gtgaggagcc cctccgcccg gcagccgccc
     4381 cgtccgggag gtggggggca gcccccaccc ggccagccac cccgcacggg agggaggtgg
     4441 ggccagcccc tgcctggcca gccgccccat tcaggaggtg gggggcagcc cctgcccggc
     4501 agccatcccg tccgggaggt ggggggcgcc tctgcccggc tgccccatct gggaagtgag
     4561 gagcccctct gcccggcagc caccccgtct gggaggtgtg cccaacagct cattgagaac
     4621 gggccatgat gacgatggcg gttttgtcga atagaaaggg gggaaatgtg gggaaaagaa
     4681 agagaaatca gattgttgcg gcgtctgtgt agaaagaagt agatatagga gactccattt
     4741 tgttctgtac taagaaaaat tcttctgcct tgggatgctg ttaatctata accttacccc
     4801 caaccccgtg ctctctgaaa catgtgctgt gtccactcag ggttaaatgg attaagggcg
     4861 gtgcaagatg tgctttgtta aacagatgct tgaaggcagc atgctcgtta agagtcatca
     4921 ccactcccta atctcaagta cccagggaca caaacactgc ggaaggctgc agggtcctct
     4981 gcctaggaaa accagagacc cttgttcaca tgtttatctg ctgaccttcc ctccactatt
     5041 gtcctatgac cctgccaaat ccccctctcc aaaaaacacc caagaatgat cggatccacg
     5101 cgtctccggc ctagggataa cagggtaatc cgctagcccc tctccctccc ccccccctaa
     5161 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5221 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5281 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5341 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5401 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5461 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5521 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5581 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc
     5641 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5701 acgatgataa tatggccaca ctagagtcga gctaccggtc gccaccatgg tgagcaaggg
     5761 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     5821 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5881 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     5941 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     6001 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6061 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     6121 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     6181 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     6241 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     6301 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     6361 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6421 ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatgaccga
     6481 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6541 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6601 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6661 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6721 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6781 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6841 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6901 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6961 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7021 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagcggccgc
     7081 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     7681 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7741 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7801 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7981 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8041 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8161 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8341 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8401 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8461 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9001 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10981 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11041 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11101 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11161 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    11221 aaaagctgga gctgc
//
