LOCUS       sequence-399640-       13896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NLRP3 fused with mNeonGreen to indicate its localization change.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 13896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: NLRP3-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..13896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3727..3736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3733..4128
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4144..4200
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             8300..8480
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10008..10027)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10241..10260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10360..10382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10420..10438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10506..10610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10611..10679,10680..11471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10829..10848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11642..12230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12131..12150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12384..12401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12471..12491)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12476..12805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12656..12791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccgcca ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     3781 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     3841 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     3901 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     3961 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4021 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     4081 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggccg tacgggaagc
     4141 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
     4201 ggatccatga agatggcaag cacccgctgc aagctggcca ggtacctgga ggacctggag
     4261 gatgtggact tgaagaaatt taagatgcac ttagaggact atcctcccca gaagggctgc
     4321 atccccctcc cgaggggtca gacagagaag gcagaccatg tggatctagc cacgctaatg
     4381 atcgacttca atggggagga gaaggcgtgg gccatggccg tgtggatctt cgctgcgatc
     4441 aacaggagag acctttatga gaaagcaaaa agagatgagc cgaagtgggg ttcagataat
     4501 gcacgtgttt cgaatcccac tgtgatatgc caggaagaca gcattgaaga ggagtggatg
     4561 ggtttactgg agtacctttc gagaatctct atttgtaaaa tgaagaaaga ttaccgtaag
     4621 aagtacagaa agtacgtgag aagcagattc cagtgcattg aagacaggaa tgcccgtctg
     4681 ggtgagagtg tgagcctcaa caaacgctac acacgactgc gtctcatcaa ggagcaccgg
     4741 agccagcagg agagggagca ggagcttctg gccatcggca agaccaagac gtgtgagagc
     4801 cccgtgagtc ccattaagat ggagttgctg tttgaccccg atgatgagca ttctgagcct
     4861 gtgcacaccg tggtgttcca gggggcggca gggattggga aaacaatcct ggccaggaag
     4921 atgatgttgg actgggcatc ggggacactc taccaagaca ggtttgacta tctgttctat
     4981 atccactgtc gggaggtgag ccttgtgaca cagaggagcc tgggggacct gatcatgagc
     5041 tgctgccccg acccaaaccc acccatccac aagatcgtga gaaaaccctc cagaatcctc
     5101 ttcctcatgg acggcttcga tgagctgcaa ggtgcctttg acgagcacat aggaccgctc
     5161 tgcactgact ggcagaaggc cgagcgggga gacattctcc tgagcagcct catcagaaag
     5221 aagctgcttc ccgaggcctc tctgctcatc accacgagac ctgtggccct ggagaaactg
     5281 cagcacttgc tggaccatcc tcggcatgtg gagatcctgg gtttctccga ggccaaaagg
     5341 aaagagtact tcttcaagta cttctctgat gaggcccaag ccagggcagc cttcagtctg
     5401 attcaggaga acgaggtcct cttcaccatg tgcttcatcc ccctggtctg ctggatcgtg
     5461 tgcactggac tgaaacagca gatggagagt ggcaagagcc ttgcccagac atccaagacc
     5521 accaccgcgg tgtacgtctt cttcctttcc agtttgctgc agccccgggg agggagccag
     5581 gagcacggcc tctgcgccca cctctggggg ctctgctctt tggctgcaga tggaatctgg
     5641 aaccagaaaa tcctgtttga ggagtccgac ctcaggaatc atggactgca gaaggcggat
     5701 gtgtctgctt tcctgaggat gaacctgttc caaaaggaag tggactgcga gaagttctac
     5761 agcttcatcc acatgacttt ccaggagttc tttgccgcca tgtactacct gctggaagag
     5821 gaaaaggaag gaaggacgaa cgttccaggg agtcgtttga agcttcccag ccgagacgtg
     5881 acagtccttc tggaaaacta tggcaaattc gaaaaggggt atttgatttt tgttgtacgt
     5941 ttcctctttg gcctggtaaa ccaggagagg acctcctact tggagaagaa attaagttgc
     6001 aagatctctc agcaaatcag gctggagctg ctgaaatgga ttgaagtgaa agccaaagct
     6061 aaaaagctgc agatccagcc cagccagctg gaattgttct actgtttgta cgagatgcag
     6121 gaggaggact tcgtgcaaag ggccatggac tatttcccca agattgagat caatctctcc
     6181 accagaatgg accacatggt ttcttccttt tgcattgaga actgtcatcg ggtggagtca
     6241 ctgtccctgg ggtttctcca taacatgccc aaggaggaag aggaggagga aaaggaaggc
     6301 cgacaccttg atatggtgca gtgtgtcctc ccaagctcct ctcatgctgc ctgttctcat
     6361 ggattggtga acagccacct cacttccagt ttttgccggg gcctcttttc agttctgagc
     6421 accagccaga gtctaactga attggacctc agtgacaatt ctctggggga cccagggatg
     6481 agagtgttgt gtgaaacgct ccagcatcct ggctgtaaca ttcggagatt gtggttgggg
     6541 cgctgtggcc tctcgcatga gtgctgcttc gacatctcct tggtcctcag cagcaaccag
     6601 aagctggtgg agctggacct gagtgacaac gccctcggtg acttcggaat cagacttctg
     6661 tgtgtgggac tgaagcacct gttgtgcaat ctgaagaagc tctggttggt cagctgctgc
     6721 ctcacatcag catgttgtca ggatcttgca tcagtattga gcaccagcca ttccctgacc
     6781 agactctatg tgggggagaa tgccttggga gactcaggag tcgcaatttt atgtgaaaaa
     6841 gccaagaatc cacagtgtaa cctgcagaaa ctggggttgg tgaattctgg ccttacgtca
     6901 gtctgttgtt cagctttgtc ctcggtactc agcactaatc agaatctcac gcacctttac
     6961 ctgcgaggca acactctcgg agacaagggg atcaaactac tctgtgaggg actcttgcac
     7021 cccgactgca agcttcaggt gttggaatta gacaactgca acctcacgtc acactgctgc
     7081 tgggatcttt ccacacttct gacctccagc cagagcctgc gaaagctgag cctgggcaac
     7141 aatgacctgg gcgacctggg ggtcatgatg ttctgtgaag tgctgaaaca gcagagctgc
     7201 ctcctgcaga acctggggtt gtctgaaatg tatttcaatt atgagacaaa aagtgcgtta
     7261 gaaacacttc aagaagaaaa gcctgagctg accgtcgtct ttgagccttc ttggactagt
     7321 atggtcagca aaggcgaaga agacaacatg gcatcactgc cagcgacaca cgaacttcat
     7381 attttcggct ccatcaatgg tgtggacttc gatatggttg gacagggtac tgggaaccct
     7441 aacgatggat atgaggagtt gaacttgaag agtaccaagg gcgatcttca gttttcacct
     7501 tggattctcg tacctcatat aggttatggc tttcatcaat atctgcccta tcctgacggt
     7561 atgtcacctt tccaagcagc tatggttgac gggagcggtt atcaggttca ccggacaatg
     7621 cagtttgaag atggagcctc tttgaccgta aattatcggt atacttatga gggcagccat
     7681 attaaaggcg aggctcaagt taaggggact gggttccccg cggatggtcc cgtaatgaca
     7741 aacagtctga cggctgcaga ctggtgccgc tctaagaaga catatccaaa tgacaagacg
     7801 attattagca ctttcaagtg gtcatacact acaggaaatg gtaaacgcta taggagcact
     7861 gcccggacca cgtatacttt cgcgaagccg atggcggcaa attatttgaa aaaccaaccg
     7921 atgtatgtgt tcagaaagac agaactcaag cattctaaaa ctgaactgaa cttcaaagag
     7981 tggcagaaag cctttactga tgtaatgggc atggacgaac tctataagta actcgagacc
     8041 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     8101 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     8161 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8221 tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca
     8281 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8341 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8401 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8461 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8521 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa
     8581 ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga
     8641 gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt
     8701 actaacattt atataatgat ttatagttta aagtataaac ttatctaatt tactattctg
     8761 acagatatta attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca
     8821 caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt
     8881 agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt
     8941 cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa
     9001 tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga
     9061 agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa
     9121 gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa
     9181 ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa
     9241 gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat
     9301 tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa
     9361 gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag
     9421 agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt
     9481 gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact
     9541 ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt
     9601 tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag
     9661 atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat
     9721 ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat
     9781 ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta
     9841 aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt
     9901 tatttagcat ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg
     9961 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
    10021 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
    10081 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
    10141 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
    10201 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
    10261 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    10321 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    10381 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
    10441 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    10501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    12481 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    12541 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    12601 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    12661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    12721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    12781 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
    12841 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    12901 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    12961 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    13021 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    13081 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    13141 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    13201 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    13261 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    13321 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    13381 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    13441 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    13501 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    13561 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    13621 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    13681 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    13741 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    13801 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    13861 cggatctttg tgaaggaacc ttacttctgt ggtgtg
//
