LOCUS       sequence-399642-       11310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin 
            (SEP) and mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: GALT-SEP-mRuby3
FEATURES             Location/Qualifiers
     source          1..11310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4009..4716
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             4729..5445
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4759..4787)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5382..5402
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     LTR             5714..5894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7422..7441)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7655..7674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7774..7796
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7834..7852)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7920..8024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8025..8093,8094..8885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8243..8262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9056..9644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9545..9564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9798..9815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9885..9905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9890..10219
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10070..10205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccgcca ccatgaggct tcgggagccg ctcctgagcg gcagcgccgc gatgccaggc
     3781 gcgtccctac agcgggcctg ccgcctgctc gtggccgtct gcgctctgca ccttggcgtc
     3841 accctcgttt actacctggc tggccgcgac ctgagccgcc tgccccaact ggtcggagtc
     3901 tccacaccgc tgcagggcgg ctcgaacagt gccgccgcca tcgggcagtc ctccggggag
     3961 ctccggaccg gaggggccgt ggatccaggc ggcagcggcg gcagcgctgt gtctaagggc
     4021 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
     4081 caccaattca aatgcacagg tgaaggagaa ggcagaccgt acgagggagt gcaaaccatg
     4141 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
     4201 ttcatgtatg gcagccgtac ctttatcaag tacccggccg acatccctga tttctttaaa
     4261 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc
     4321 gtcaccgtca cgcaggacac cagccttgag gatggcgagc tcgtctacaa cgtcaaggtc
     4381 agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag
     4441 cctaatacag agatgatgta tccagcagat ggtggtctga gaggatacac tgacatcgca
     4501 ctgaaagttg atggtggtgg ccatctgcac tgcaacttcg tgacaactta caggtcaaaa
     4561 aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg ttgatcaccg cctggaaagg
     4621 atcgaggaga gtgacaatga aacctacgta gtgcaaagag aagtggcagt tgccaaatac
     4681 agcaaccttg gtggtggcat ggacgagctg tacaagggat ccgccaccat gagtaaagga
     4741 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     4801 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     4861 aaatttattt gcactactgg aaaactacct gttccttggc caacacttgt cactacttta
     4921 acttatggtg ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc
     4981 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     5041 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     5101 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     5161 tataacgatc accaggtgta catcatggca gacaaacaaa agaatggaat caaagctaac
     5221 ttcaaaatta gacacaacat tgaagatgga ggcgttcaac tagcagacca ttatcaacaa
     5281 aatactccaa ttggcgatgg gcccgtcctt ttaccagaca accattacct gtttacaact
     5341 tctactcttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     5401 acagctgctg ggattacaca tggcatggat gaactataca aataactcga gacctagaaa
     5461 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     5521 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     5581 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5641 ggctaattca ctcccaacga agacaaaatc gtcgagagat gctgcatata agcagctgct
     5701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5941 gaaatgaata tcagagagtg agaggccttg acattataat agatttagca ggaattgaac
     6001 taggagtgga gcacacaggc aaagctgcag aagtacttgg aagaagccac cagagatact
     6061 cacgattctg cacatacctg gctaatccca gatcctaagg attacattaa gtttactaac
     6121 atttatataa tgatttatag tttaaagtat aaacttatct aatttactat tctgacagat
     6181 attaattaat cctcaaatat cataagagat gattactatt atccccattt aacacaagag
     6241 gaaactgaga gggaaagatg ttgaagtaat tttcccacaa ttacagcatc cgttagttac
     6301 gactctatga tcttctgaca caaattccat ttactcctca ccctatgact cagtcgaata
     6361 tatcaaagtt atggacatta tgctaagtaa caaattaccc ttttatatag taaatactga
     6421 gtagattgag agaagaaatt gtttgcaaac ctgaatagct tcaagaagaa gagaagtgag
     6481 gataagaata acagttgtca tttaacaagt tttaacaagt aacttggtta gaaagggatt
     6541 caaatgcata aagcaaggga taaatttttc tggcaacaag actatacaat ataaccttaa
     6601 atatgacttc aaataattgt tggaacttga taaaactaat taaatattat tgaagattat
     6661 caatattata aatgtaattt acttttaaaa agggaacata gaaatgtgta tcattagagt
     6721 agaaaacaat ccttattatc acaatttgtc aaaacaagtt tgttattaac acaagtagaa
     6781 tactgcattc aattaagttg actgcagatt ttgtgttttg ttaaaattag aaagagataa
     6841 caacaatttg aattattgaa agtaacatgt aaatagttct acatacgttc ttttgacatc
     6901 ttgttcaatc attgatcgaa gttctttatc ttggaagaat ttgttccaaa gactctgaaa
     6961 taaggaaaac aatctattat atagtctcac acctttgttt tacttttagt gatttcaatt
     7021 taataatgta aatggttaaa atttattctt ctctgagatc atttcacatt gcagatagaa
     7081 aacctgagac tggggtaatt tttattaaaa tctaatttaa tctcagaaac acatctttat
     7141 tctaacatca atttttccag tttgatatta tcatataaag tcagccttcc tcatctgcag
     7201 gttccacaac aaaaatccaa ccaactgtgg atcaaaaata ttgggaaaaa attaaaaata
     7261 gcaatacaac aataaaaaaa tacaaatcag aaaaacagca cagtataaca actttattta
     7321 gcatttacaa tctattaggt attataagta atctagccag atcctctacg ccggacgcat
     7381 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     7441 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     7501 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     7561 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     7621 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7681 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7741 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7801 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     7861 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     7921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     9901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    10201 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    10261 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    10321 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    10381 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    10441 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    10501 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    10561 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    10621 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    10681 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    10741 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    10801 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    10861 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    10921 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    10981 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    11041 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    11101 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    11161 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    11221 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    11281 tttgtgaagg aaccttactt ctgtggtgtg
//
