LOCUS       sequence-399647-       12165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STING fused with miRFP680 for live cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 12165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: STING-miRFP680
FEATURES             Location/Qualifiers
     source          1..12165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3727..3736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3733..4128
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4144..4200
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5350..6300
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE"
     LTR             6569..6749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8277..8296)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8510..8529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8629..8651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8689..8707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8775..8879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8880..8948,8949..9740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9098..9117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9911..10499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10400..10419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10653..10670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10740..10760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10745..11074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10925..11060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccgcca ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     3781 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     3841 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     3901 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     3961 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4021 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     4081 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggccg tacgggaagc
     4141 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct
     4201 ggatccatgc cccactccag cctgcatcca tccatcccgt gtcccagggg tcacggggcc
     4261 cagaaggcag ccttggttct gctgagtgcc tgcctggtga ccctttgggg gctaggagag
     4321 ccaccagagc acactctccg gtacctggtg ctccacctag cctccctgca gctgggactg
     4381 ctgttaaacg gggtctgcag cctggctgag gagctgcgcc acatccactc caggtaccgg
     4441 ggcagctact ggaggactgt gcgggcctgc ctgggctgcc ccctccgccg tggggccctg
     4501 ttgctgctgt ccatctattt ctactactcc ctcccaaatg cggtcggccc gcccttcact
     4561 tggatgcttg ccctcctggg cctctcgcag gcactgaaca tcctcctggg cctcaagggc
     4621 ctggccccag ctgagatctc tgcagtgtgt gaaaaaggga atttcaacgt ggcccatggg
     4681 ctggcatggt catattacat cggatatctg cggctgatcc tgccagagct ccaggcccgg
     4741 attcgaactt acaatcagca ctataataat ctcctgcggg gtgcagtgag ccagcggctg
     4801 tatattctcc tcccattgga ctgtggggtg cctgataacc tgagtatggc tgaccccaac
     4861 attcgcttcc tggataaact gccccagcag accggtgacc gtgctggcat caaggatcgg
     4921 gtttacagca acagcatcta tgagcttctg gagaacgggc agcgggcggg cacctgtgtc
     4981 ctggagtacg ccaccccctt gcagactttg tttgccatgt cacaatacag tcaagctggc
     5041 tttagccggg aggataggct tgagcaggcc aaactcttct gccggacact tgaggacatc
     5101 ctggcagatg cccctgagtc tcagaacaac tgccgcctca ttgcctacca ggaacctgca
     5161 gatgacagca gcttctcgct gtcccaggag gttctccggc acctgcggca ggaggaaaag
     5221 gaagaggtta ctgtgggcag cttgaagacc tcagcggtgc ccagtacctc cacgatgtcc
     5281 caagagcctg agctcctcat cagtggaatg gaaaagcccc tccctctccg cacggatttc
     5341 tcttctagaa tggcggaagg ctccgtcgcc aggcagcctg acctcttgac ctgcgacgat
     5401 gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc
     5461 gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc
     5521 gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg
     5581 atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg
     5641 cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag
     5701 ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc
     5761 gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa
     5821 gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc
     5881 agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac
     5941 tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg
     6001 atcattcccg atatcaatta tcggccggtg ccggtcaccc cagacctcaa tccggtcacc
     6061 gggcggccga ttgatcttag cttcgccatc ctgcgcagcg tctcgccctg ccatctggag
     6121 ttcatgcgca acataggcat gcacggcacg atgtcgatct cgattttgcg cggcgagcga
     6181 ctgtggggat tgatcgtttg ccatcaccga acgccgtact acgtcgatct cgatggccgc
     6241 caagcctgca agagggtcgc cgagaggctg gccactcaga tcggcgtgat ggaagagtaa
     6301 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6361 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6421 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6481 aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga gagatgctgc
     6541 atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6781 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt ataatagatt
     6841 tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta cttggaagaa
     6901 gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc taaggattac
     6961 attaagttta ctaacattta tataatgatt tatagtttaa agtataaact tatctaattt
     7021 actattctga cagatattaa ttaatcctca aatatcataa gagatgatta ctattatccc
     7081 catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc cacaattaca
     7141 gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact cctcacccta
     7201 tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat taccctttta
     7261 tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa tagcttcaag
     7321 aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa caagtaactt
     7381 ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca acaagactat
     7441 acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa ctaattaaat
     7501 attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga acatagaaat
     7561 gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac aagtttgtta
     7621 ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg ttttgttaaa
     7681 attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata gttctacata
     7741 cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga agaatttgtt
     7801 ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt tgttttactt
     7861 ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg agatcatttc
     7921 acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa tttaatctca
     7981 gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata taaagtcagc
     8041 cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa aaatattggg
     8101 aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa cagcacagta
     8161 taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta gccagatcct
     8221 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     8281 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     8341 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     8401 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     8461 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     8521 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     8581 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     8641 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     8701 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8761 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8821 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8881 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8941 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9001 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9061 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
    10741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10801 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10981 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    11041 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    11101 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    11161 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    11221 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    11281 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    11341 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    11401 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    11461 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    11521 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    11581 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    11641 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    11701 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    11761 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    11821 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    11881 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    11941 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    12001 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    12061 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    12121 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtg
//
