LOCUS       sequence-399651-       10390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express of SVA-lncRNA AK057321 with CMV for 
            mcherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson 
            M, Moreno A, Matthew P Anderson
  TITLE     Hominoid SVA-lncRNA AK057321 targets human-specific SVA 
            retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal 
            maturation.
  JOURNAL   Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8.
  PUBMED    36997626
REFERENCE   2  (bases 1 to 10390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-03-30"; volume: "6(1):347. doi"; pages: " 
            10.1038/s42003-023-04683-8"
COMMENT     Sequence Label: pLVX-SVA-lncRNA-AK057321-mcherry
FEATURES             Location/Qualifiers
     source          1..10390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2196..2444
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2196..2216
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2367..2386
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        4287..4590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4591..4794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4745..4765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4833..4842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4839..5549
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4984..5002)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5252..5271
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5582..6170
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5635..5655)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6053..6064)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6377..7010
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7138..7154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7138..7154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7151..7173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7162..7178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7186..7192,7193..7210,7211..7216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7231..7252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7369..7386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7540..8128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7620..7639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8299..9090,9091..9159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8922..8941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     mobile_element  9200..9967
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     polyA_signal    10089..10223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10126..10145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10180..10199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt
     2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccgagcag ccctgaggat
     2461 ggcggcggcg gcggccagca ccggccggtg ggcgggagcc ccggcgggcc gcgcctggtg
     2521 atcggctcct taccagctca cctctcaccg cgcatgtttg gagactatat catcatgtac
     2581 atagaaaaat ccaaaggatt ctgcaaaaaa gctactagaa ccactgactt catcaagatg
     2641 cagtctccct ctgatgccga gcggaggctg gactgtacag ccaccatctc ggctcactgc
     2701 aacatccctg cctgattctc ctgcctcagc ctgccgagtg cctgggattg caggctcgcg
     2761 ccgccacgcc tgactggttt ttgtattttt tggtggagaa ggggtttcgc catgttggct
     2821 gggctggtct ccagctcctg accgcaagtg atctgcccgc ctcggcctcc cgaggtgcca
     2881 ggattgcaga cggagtctcg ctcactcagt gctcaatgtt gcccaggctg gagtgcagtg
     2941 gcgtgatctc agctcgctac aacctccacc tcccagccgc ctgccttggc ctcccaaagt
     3001 gctgagattg cagcctctgc ccggccgcca ccccgtctgg gaagtgagga gcgtctctgc
     3061 ctggccgccc atcgtctggg atgtgaggag cccctctgcc cggccgccca gtctgagatg
     3121 tgaagagcgc ctctgcccgg ccgcgacccc gtctgggaac tgaggagtgt ctctgcctgg
     3181 ccacccatcg tctgggatgt gaggagcccc tctgcccggc cgcccagtct gggaaatgag
     3241 gagcgcctct tcctggccac catcccgtct gggaagtgag gagcgtctct gcccggccgc
     3301 ccatcgtctg agatgtgggg agcgcctctg cccggcccca accccgtctg ggaactgagg
     3361 agtgtctctg cccgaccgcc accccgtctg ggaggtgagg aacgtttctg cccagccgcc
     3421 cgtctgagaa gtgagcccct ccgcctggca gccgccccat ctgggaagtg aggagcccct
     3481 ccgcccggca gccgccccgt ccgggaggtg gggggcagcc cccacccggc cagccacccc
     3541 gcacgggagg gaggtggggc cagcccctgc ctggccagcc gccccattca ggaggtgggg
     3601 ggcagcccct gcccggcagc catcccgtcc gggaggtggg gggcgcctct gcccggctgc
     3661 cccatctggg aagtgaggag cccctctgcc cggcagccac cccgtctggg aggtgtgccc
     3721 aacagctcat tgagaacggg ccatgatgac gatggcggtt ttgtcgaata gaaagggggg
     3781 aaatgtgggg aaaagaaaga gaaatcagat tgttgcggcg tctgtgtaga aagaagtaga
     3841 tataggagac tccattttgt tctgtactaa gaaaaattct tctgccttgg gatgctgtta
     3901 atctataacc ttacccccaa ccccgtgctc tctgaaacat gtgctgtgtc cactcagggt
     3961 taaatggatt aagggcggtg caagatgtgc tttgttaaac agatgcttga aggcagcatg
     4021 ctcgttaaga gtcatcacca ctccctaatc tcaagtaccc agggacacaa acactgcgga
     4081 aggctgcagg gtcctctgcc taggaaaacc agagaccctt gttcacatgt ttatctgctg
     4141 accttccctc cactattgtc ctatgaccct gccaaatccc cctctccaaa aaacacccaa
     4201 gaatgatctt ttttacgcgt gaattctagt tattaatagt aatcaattac ggggtcatta
     4261 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4321 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4381 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4441 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4501 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4561 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4621 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4681 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4741 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     4801 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg
     4861 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     4921 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     4981 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     5041 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     5101 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     5161 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     5221 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     5281 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     5341 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     5401 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     5461 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     5521 ccggcggcat ggacgagctg tacaagtagc tcgagtctag agggcccgac gcgtctggaa
     5581 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5641 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5701 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5761 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5821 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5881 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5941 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6001 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6061 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6121 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     6181 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     6241 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     6301 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6361 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     6421 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     6481 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     6541 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     6601 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     6661 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     6721 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     6781 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     6841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7021 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7081 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7141 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7201 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7321 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7381 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7441 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8881 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9181 ttgaagcatt tatcagggtg gtaatgactc caacttattg atagtgtttt atgttcagat
     9241 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     9301 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     9361 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     9421 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     9481 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     9541 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     9601 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     9661 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     9721 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     9781 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     9841 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     9901 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     9961 catcacctat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10081 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10141 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10201 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10261 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    10321 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    10381 acttagtagt
//
