LOCUS       sequence-399652-        8923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent mitophagy reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pCLBW cox8 EGFP mCherry
FEATURES             Location/Qualifiers
     source          1..8923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          175..1191
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1115..1137
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1199..1218
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1279..1303
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1310..1384
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(1406..1408,1409..2119)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1406..1408,1409..2119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1448..1469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1709..1728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2056..2077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2135..2845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2280..2298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2548..2567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2846..2870)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2915..3503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2968..2988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3386..3397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3506..3522)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3507..3523)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3840..4309
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4439..4456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4610..5198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4690..4709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5358..6149,6150..6218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5981..6000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     enhancer        6370..6749
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6750..6953
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6904..6924
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    7192..7549
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7614..8030
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7960..7982
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7998..8014
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    8040..8414
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     8352..8368
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     enhancer        8515..8818
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcgggggggg ggggggggsg cgcgccgggg gggggggggg gggggggggg gggggggggg
       61 gsgrggsggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      121 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      181 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      241 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      301 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
      361 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
      421 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
      481 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
      541 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
      601 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
      661 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
      721 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
      781 ccycgggccg gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt
      841 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
      901 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
      961 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1021 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1081 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1141 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1201 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgatttg ataccgcggg
     1261 cccgggatcc ctcgaatcac aagtttgtac aaaaaagcag gctgacacca tgtccgtcct
     1321 gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc
     1381 caagatccat tcgttggggg atccggtgag caagggcgag gagctgttca ccggggtggt
     1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1981 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2101 catggacgag ctgtacaagg gctcaggctc gggcatggtg agcaagggcg aggaggataa
     2161 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     2221 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2281 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     2341 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     2401 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     2461 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2521 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2581 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     2641 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     2701 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     2761 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     2821 cggcatggac gagctgtata agtaaaccca gctttcttgt acaaagtggt gattcgaggc
     2881 cgcgactcta gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2941 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3001 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3061 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3121 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3181 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3241 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3301 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3361 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3421 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3481 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagga attccagctg
     3541 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     3601 ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actattttcg agcggccgcc
     3661 agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     3721 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     3781 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     3841 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     3901 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     3961 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4021 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4081 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     4141 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     4201 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     4261 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     4321 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     4381 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4441 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4501 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4561 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4621 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4681 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4741 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4801 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4861 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4921 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4981 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5041 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5101 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5161 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5221 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5281 caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag
     5341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatgatcc
     6241 ggccattagc catattattc attggttata tagcataaat caatattggc tattggccat
     6301 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
     6361 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     6421 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6481 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     6541 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     6601 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6661 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     6721 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     6781 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     6841 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     6901 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcaataaa
     6961 agagcccaca acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac
     7021 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     7081 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     7141 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
     7201 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
     7261 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
     7321 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
     7381 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
     7441 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
     7501 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
     7561 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
     7621 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     7681 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     7741 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     7801 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     7861 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     7921 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     7981 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
     8041 catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
     8101 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
     8161 acgaagtctg gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac
     8221 ctcaccctta ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag
     8281 aacctcgctg gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag
     8341 acggcatcgc agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac
     8401 catcctctag actgccggat cccagtgtgg tggtacggga attctgcagg aatctagtta
     8461 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8521 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8581 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8641 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     8701 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     8761 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     8821 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     8881 tttgtattta tttatttttt aattattttg tgcagcgatg ggg
//
