LOCUS       sequence-399655-       11330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cis/medial golgi localized pH sensor using sensorsuperecliptic 
            pHluorin (SEP) and mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 11330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: MGAT-SEP-mRuby3
FEATURES             Location/Qualifiers
     source          1..11330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4030..4737
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             4750..5463
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4780..4808)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5403..5423
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     LTR             5734..5914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7442..7461)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7675..7694)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7794..7816
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7854..7872)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7940..8044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8045..8113,8114..8905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8263..8282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9076..9664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9565..9584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9818..9835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9905..9925)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9910..10239
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10090..10225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccgcca ccatgcgctt ccgcatctac aagcgcaagg tgctgatcct gaccctggtg
     3781 gtggccgcct gcggcttcgt gctgtggagc agcaacggcc gccagcgcaa gaacgaggcc
     3841 ctggcccccc ccctgctgga cgccgagccc gcccgcggcg ccggcggccg cggcggcgac
     3901 caccccagcg tggccgtggg catccgccgc gtgagcaacg tgagcgccgc cagcctggtg
     3961 cccgccgtgc cccagcccga ggccgacaac ctgaccctgt tggatccagg cggcagcggc
     4021 ggcagcgctg tgtctaaggg cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc
     4081 atggaaggtt cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcagaccg
     4141 tacgagggag tgcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc
     4201 tttgacattc ttgccacgtc gttcatgtat ggcagccgta cctttatcaa gtacccggcc
     4261 gacatccctg atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg
     4321 agatacgaag atggtggagt cgtcaccgtc acgcaggaca ccagccttga ggatggcgag
     4381 ctcgtctaca acgtcaaggt cagaggggta aactttccct ccaatggtcc cgtgatgcag
     4441 aagaagacca agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg
     4501 agaggataca ctgacatcgc actgaaagtt gatggtggtg gccatctgca ctgcaacttc
     4561 gtgacaactt acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tgtccatgcc
     4621 gttgatcacc gcctggaaag gatcgaggag agtgacaatg aaacctacgt agtgcaaaga
     4681 gaagtggcag ttgccaaata cagcaacctt ggtggtggca tggacgagct gtacaaggga
     4741 tccgccacca tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     4801 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     4861 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccttgg
     4921 ccaacacttg tcactacttt aacttatggt gttcaatgct tttcaagata cccagatcat
     4981 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     5041 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     5101 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     5161 ggacacaaat tggaatacaa ctataacgat caccaggtgt acatcatggc agacaaacaa
     5221 aagaatggaa tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg aggcgttcaa
     5281 ctagcagacc attatcaaca aaatactcca attggcgatg ggcccgtcct tttaccagac
     5341 aaccattacc tgtttacaac ttctactctt tcgaaagatc ccaacgaaaa gagagaccac
     5401 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     5461 aaaaactcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5521 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5581 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5641 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaaaatc gtcgagagat
     5701 gctgcatata agcagctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5761 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5821 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5881 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     5941 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat
     6001 agatttagca ggaattgaac taggagtgga gcacacaggc aaagctgcag aagtacttgg
     6061 aagaagccac cagagatact cacgattctg cacatacctg gctaatccca gatcctaagg
     6121 attacattaa gtttactaac atttatataa tgatttatag tttaaagtat aaacttatct
     6181 aatttactat tctgacagat attaattaat cctcaaatat cataagagat gattactatt
     6241 atccccattt aacacaagag gaaactgaga gggaaagatg ttgaagtaat tttcccacaa
     6301 ttacagcatc cgttagttac gactctatga tcttctgaca caaattccat ttactcctca
     6361 ccctatgact cagtcgaata tatcaaagtt atggacatta tgctaagtaa caaattaccc
     6421 ttttatatag taaatactga gtagattgag agaagaaatt gtttgcaaac ctgaatagct
     6481 tcaagaagaa gagaagtgag gataagaata acagttgtca tttaacaagt tttaacaagt
     6541 aacttggtta gaaagggatt caaatgcata aagcaaggga taaatttttc tggcaacaag
     6601 actatacaat ataaccttaa atatgacttc aaataattgt tggaacttga taaaactaat
     6661 taaatattat tgaagattat caatattata aatgtaattt acttttaaaa agggaacata
     6721 gaaatgtgta tcattagagt agaaaacaat ccttattatc acaatttgtc aaaacaagtt
     6781 tgttattaac acaagtagaa tactgcattc aattaagttg actgcagatt ttgtgttttg
     6841 ttaaaattag aaagagataa caacaatttg aattattgaa agtaacatgt aaatagttct
     6901 acatacgttc ttttgacatc ttgttcaatc attgatcgaa gttctttatc ttggaagaat
     6961 ttgttccaaa gactctgaaa taaggaaaac aatctattat atagtctcac acctttgttt
     7021 tacttttagt gatttcaatt taataatgta aatggttaaa atttattctt ctctgagatc
     7081 atttcacatt gcagatagaa aacctgagac tggggtaatt tttattaaaa tctaatttaa
     7141 tctcagaaac acatctttat tctaacatca atttttccag tttgatatta tcatataaag
     7201 tcagccttcc tcatctgcag gttccacaac aaaaatccaa ccaactgtgg atcaaaaata
     7261 ttgggaaaaa attaaaaata gcaatacaac aataaaaaaa tacaaatcag aaaaacagca
     7321 cagtataaca actttattta gcatttacaa tctattaggt attataagta atctagccag
     7381 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     7441 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     7501 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     7561 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     7621 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     7681 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     7741 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7801 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     7861 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9721 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9781 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9841 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9901 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9961 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10081 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10201 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    10261 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
    10321 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
    10381 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
    10441 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
    10501 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
    10561 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
    10621 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
    10681 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
    10741 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
    10801 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
    10861 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
    10921 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
    10981 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
    11041 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
    11101 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
    11161 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
    11221 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    11281 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
//
