LOCUS       sequence-399665-        9019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0969
FEATURES             Location/Qualifiers
     source          1..9019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1957..2545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2010..2030)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2428..2439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2617..2850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2928..3049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2965..2984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3019..3038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3089..3224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3244..3263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3245..3263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3273..3290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3273..3289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3282..3304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3431..3886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3518..3537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3728..3749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3912..4016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4017..4085,4086..4877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4235..4254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5048..5636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5537..5556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5790..5807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5924..5945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5960..5965,5966..5983,5984..5990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5998..6014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6003..6025
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6022..6038
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6022..6038
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6057..6077
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6059..6077
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6105..6331
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6332..6512
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6559..6684
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7177..7410
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7595..7639
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7870..8080
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7892..8088
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7939..8074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8120..8722
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8120..8139)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8616..8636
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8765..8882
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 tctggtaggt ggttttcccc accacccggt ggtgcaccac gagggctacc cgtttgccgc
     1261 cgccgccgcc gcagctgccg ccgccgccgc cagccgctgc agccatgagg agaaccccta
     1321 cttccatggc tggctcatcg gccaccccga gatgtcgccc cccgactaca gcatggccct
     1381 gtcctacagc cccgagtatg ccagcggcgc cgccggcctg gaccactccc attacggggg
     1441 ggtgccgccg ggcgccgggc ccccgggcct gggggggccg cgcccggtga agcgccgagg
     1501 caccgccaac cgcaaggagc ggcgcaggac tcagagcatc aacagcgcct tcgccgaact
     1561 gcgcgagtgc atccccaacg tacccgccga caccaaactc tccaaaatca agaccctgcg
     1621 cctggccacc agctacatcg cctacctcat ggacctgctg gccaaggacg accagaatgg
     1681 cgaggcggag gccttcaagg cagagatcaa gaagaccgac gtgaaagagg agaagaggaa
     1741 gaaggagctg aacgaaatct tgaaaagcac agtgagcagc aacgacaaga aaaccaaagg
     1801 ccggacgggc tggccgcagc acgtctgggc cctggagctc aagcagtagt aatgaactag
     1861 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaaa
     1921 gtattgctgt ctgtagtacc tacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     1981 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2041 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2101 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2161 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2221 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2281 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2341 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2401 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2461 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2521 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     2581 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2641 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2821 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     2881 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     2941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3061 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3121 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3181 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3241 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3301 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3421 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3481 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3541 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3601 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3661 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3721 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3781 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3841 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4561 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5161 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5821 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5881 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5941 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6001 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6061 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6121 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6181 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6241 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6301 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6361 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6421 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6481 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6541 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6601 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6661 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6721 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6781 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6841 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6901 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6961 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7021 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7081 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7141 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7201 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7261 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7321 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7381 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7441 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7501 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7561 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     7621 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7681 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7741 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7801 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7861 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     7921 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7981 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8041 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8101 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8161 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8221 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8281 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     8341 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8401 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8461 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8521 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8581 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8641 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8701 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     8761 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8821 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     8881 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     8941 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     9001 cttgaaagga gtgggaatt
//
