LOCUS       sequence-399668-       11756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RFP fused with LC3B for reporting LC3B lipidation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Liu B, Carlson RJ, Pires IS, Gentili M, Feng E, Hellier Q, Schwartz 
            MA, Blainey PC, Irvine DJ, Hacohen N
  TITLE     Human STING is a proton channel.
  JOURNAL   Science. 2023 Aug 4;381(6657):508-514. doi: 10.1126/science.adf8974.
            Epub 2023 Aug 3.
  PUBMED    37535724
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adf8974"; journalName: "Science"; date: "2023-08-4-
            4"; volume: "381"; issue: "6657"; pages: "508-514"
COMMENT     Sequence Label: RFP-LC3B
FEATURES             Location/Qualifiers
     source          1..11756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(377..397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        382..711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      562..697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1845..1910
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2040..2060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2485..2619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2522..2541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2576..2595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2787..3420
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3467..3592
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4085..4318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4503..4547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4840..4957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5005..5515
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5434..5452
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5534..6556
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5534..6556
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6572..6628
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6635..7309
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6635..7309
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(6765..6783)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7033..7052
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7227..7250
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             7316..7690
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     LTR             7962..8142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9670..9689)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9903..9922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10022..10044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10082..10100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10168..10272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10273..10341,10342..11133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10491..10510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11304..11756,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      421 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      481 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      541 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      661 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
      721 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
      781 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
      841 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
      901 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
      961 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     1021 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     1081 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     1141 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     1201 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     1261 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     1321 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     1381 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     1441 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     1501 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     1561 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     1621 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     1681 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     1741 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     1801 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     1861 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     1921 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     1981 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     2041 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     2101 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     2161 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     2221 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     2281 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     2341 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     2401 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2461 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2581 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     2641 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     2701 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     2761 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     2821 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     2881 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     2941 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     3001 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     3061 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     3121 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     3181 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     3241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3421 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     3481 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     3541 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     3601 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     3661 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     3721 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3781 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3841 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3901 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     3961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4741 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     4801 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     4981 agatccactt tggctgatac gcgtggggtt ggggttgcgc cttttccaag gcagccctgg
     5041 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     5101 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     5161 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     5221 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     5281 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     5341 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     5401 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     5461 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagccgcg
     5521 ggctagcgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     5581 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     5641 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     5701 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     5761 agtgcttgac attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca
     5821 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     5881 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     5941 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6001 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6061 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6121 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6181 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6241 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6301 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6361 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6421 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6481 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6541 tccgagggca aaggaacgta cgggaagcgg agctactaac ttcagcctgc tgaagcaggc
     6601 tggagacgtg gaggagaacc ctggacctgg atccatggcc tcctccgagg acgtcatcaa
     6661 ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat
     6721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6781 caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc
     6841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     7021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     7081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga
     7141 cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct
     7201 gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac
     7261 catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgcct ctagaatgcc
     7321 gtcggagaag accttcaagc agcgccgcac cttcgaacaa agagtagaag atgtccgact
     7381 tattcgagag cagcatccaa ccaaaatccc ggtgataata gaacgataca agggtgagaa
     7441 gcagcttcct gttctggata aaacaaagtt ccttgtacct gaccatgtca acatgagtga
     7501 gctcatcaag ataattagaa ggcgcttaca gctcaatgct aatcaggcct tcttcctgtt
     7561 ggtgaacgga cacagcatgg tcagcgtctc cacaccaatc tcagaggtgt atgagagtga
     7621 gaaagatgaa gatggattcc tgtacatggt ctatgcctcc caggagacgt tcgggatgaa
     7681 attgtcagtg taactcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     7741 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7801 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7861 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaaaatcgt
     7921 cgagagatgc tgcatataag cagctgcttt ttgcttgtac tgggtctctc tggttagacc
     7981 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8041 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8101 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     8221 attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa agctgcagaa
     8281 gtacttggaa gaagccacca gagatactca cgattctgca catacctggc taatcccaga
     8341 tcctaaggat tacattaagt ttactaacat ttatataatg atttatagtt taaagtataa
     8401 acttatctaa tttactattc tgacagatat taattaatcc tcaaatatca taagagatga
     8461 ttactattat ccccatttaa cacaagagga aactgagagg gaaagatgtt gaagtaattt
     8521 tcccacaatt acagcatccg ttagttacga ctctatgatc ttctgacaca aattccattt
     8581 actcctcacc ctatgactca gtcgaatata tcaaagttat ggacattatg ctaagtaaca
     8641 aattaccctt ttatatagta aatactgagt agattgagag aagaaattgt ttgcaaacct
     8701 gaatagcttc aagaagaaga gaagtgagga taagaataac agttgtcatt taacaagttt
     8761 taacaagtaa cttggttaga aagggattca aatgcataaa gcaagggata aatttttctg
     8821 gcaacaagac tatacaatat aaccttaaat atgacttcaa ataattgttg gaacttgata
     8881 aaactaatta aatattattg aagattatca atattataaa tgtaatttac ttttaaaaag
     8941 ggaacataga aatgtgtatc attagagtag aaaacaatcc ttattatcac aatttgtcaa
     9001 aacaagtttg ttattaacac aagtagaata ctgcattcaa ttaagttgac tgcagatttt
     9061 gtgttttgtt aaaattagaa agagataaca acaatttgaa ttattgaaag taacatgtaa
     9121 atagttctac atacgttctt ttgacatctt gttcaatcat tgatcgaagt tctttatctt
     9181 ggaagaattt gttccaaaga ctctgaaata aggaaaacaa tctattatat agtctcacac
     9241 ctttgtttta cttttagtga tttcaattta ataatgtaaa tggttaaaat ttattcttct
     9301 ctgagatcat ttcacattgc agatagaaaa cctgagactg gggtaatttt tattaaaatc
     9361 taatttaatc tcagaaacac atctttattc taacatcaat ttttccagtt tgatattatc
     9421 atataaagtc agccttcctc atctgcaggt tccacaacaa aaatccaacc aactgtggat
     9481 caaaaatatt gggaaaaaat taaaaatagc aatacaacaa taaaaaaata caaatcagaa
     9541 aaacagcaca gtataacaac tttatttagc atttacaatc tattaggtat tataagtaat
     9601 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     9661 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     9721 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     9781 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     9841 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     9901 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     9961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    10081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10501 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10681 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10741 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10801 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10861 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10921 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10981 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11041 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11101 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcag
//
