LOCUS       sequence-399701-       19584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19584)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 19584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:LLFLS2-1
FEATURES             Location/Qualifiers
     source          1..19584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9716..9740)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9769..9858
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9892..10144
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(10573..10825)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11293..12318)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(11293..12318)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12328..12672)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12364..12384)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(12814..12831)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12814..12830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12823..12845)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12970..12989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13180..13201
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    13448..13472
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(15068..16216)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16515..17309)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17501..18268
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(18961..19584,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatgg agtctaaaag ggtcagcttc agcttaatca tagtcttatt
     6061 ttgttctttc tttttcactg ttgtattatc aactgcagaa cgaagcttag aagaagtaga
     6121 aactgaagcc ttgaaggcct ttaagaatgg catcaccagt gacactttag gagcacttgc
     6181 cgattggaat gacacaaatc aaattcatca ctgcaactgg tctggaatca cctgtaactc
     6241 ttcctcaaag catgtcactg ccattaaact cgtcgacaag cagctccaag gccagatatc
     6301 tccattcctt ggcaaccttt cagccctcca ggttcttgat ttatctttga attcattctc
     6361 acggagtatt ccagctcagc tggggcaatg ctcccagctt gctgaactaa ctctgtacta
     6421 caattctctt tcgggttcca ttccaccaga aataggatcc cttcagaatc tgcaagcaat
     6481 agacttagga aataacttct tgaatggaag catacccgaa agcatttgca actgtacttc
     6541 cttgttagcg cttggtctca ttttcaacaa tcttactggt acaatcccaa aagacatagg
     6601 aaatttgaga agccttcaaa tgtttgtagc ataccacaac aagcttgtag gatcaattcc
     6661 agtttccatg ggtagattag aggccctgca agctctagat gtgagtcaga acatgttgtc
     6721 gggaacaata cctccagaaa taggaaacct gtcagactta gaataccttc agttatttca
     6781 aaattctatt ggaggaagaa tcccgtccca actaggcaat tttacaaagc ttctcgccct
     6841 tgaactgtac accaatcagc tcaccggaag cattccctct gagcttggaa atttggctag
     6901 cttacaatca atgcatctat acgaaaatag attgaactcc acaatcccaa tttctttgtt
     6961 ccagctgaaa tctttaactc gtttaggact ctcccagaat gagttgaccg gaacattacc
     7021 ccttgagcta ggatatttaa ggtcattacg agtattgacc ctacattcca ataagttcac
     7081 cggggagatc ccttcttcat tgacaaacct gacaaattta acctatttgt caatgagctt
     7141 caattcctta acagggaagc ttccatcaaa catcgggttg ctttataacc tcgaaaatct
     7201 gaccatgaat aacaatcttc ttgagggaac cattccatct agtatcacaa actgtaccca
     7261 tctcgcatcc ataggtttgg cattcaatag gattactggg agaattccca ctggtttagg
     7321 aaagatgcaa aaccttacat tcctatcttt gtcatcaaat ggtatgtccg gagaaatccc
     7381 cgatgatctt ttcaattgct caaatcttga aattctcgat ctgagtgaaa acaatttcag
     7441 tggactgctg aaaccaggaa tcggaaagct taataatctc aagattatga aaatccgaac
     7501 aaattccttt attgggccaa ttccaccaga gatcggcaat ctgagccaac tcatgacctt
     7561 gtctcttgct gaaaatagtt tcactggtcc tattacatca gagttatcca agctttctca
     7621 ccttcaagga ctctctttgc atcacaaccg tctagaaggt gcaatgcctg acagactatt
     7681 tgaactgaag cagctaacct atcttgattt gcagcaaaac aagttgacag gtccaattcc
     7741 aaatgctgtc tcaaatcttg ggttgctttc atacttgaac ctccaaggca acaatcttaa
     7801 cgggtcaata ccaataagca tggagagtct tcatcgactt ttgaccctgg atctctctca
     7861 caaccattta acaggatcca ttccaggacc tacgattgca ggtatgggga gtatacaaat
     7921 atatttgaac ttctcataca atttcctgga gggaagtatt ccagatgagc tcggtaagct
     7981 ggaaatggca caagccattg acatttcaaa caataacctc tcagggaaaa ttcctaaaat
     8041 gcttcaaggc tgcagaaatc tgttctctct tgatctgtca ggaaataaac tttctggtgc
     8101 aatccctcca gaagtttttg gtcgagtgga tatgcttatg agcttgaacc tttcaaaaaa
     8161 tggtttagac ggtgaaattc ctgaagagtt ggcaaatttg aagcatctta gctcgttgga
     8221 cctttctcac aacaagttga caggcatcat ccctgaaagc ctggccaatc tttcctcctt
     8281 gaaacaactg aacctttctt tcaatcaact tgaaggccgt attcctgaat ccggcatatt
     8341 cagaagcatt aacatgtcta atttggatgg aaacccagct ctgtgcggaa caaaaactct
     8401 cagagcatgc agctcaacga ggaaaaactc ccatcatttg tccaagagga caaagttggt
     8461 acttggctgc tcagttgctg ttgctctggt tctagttctc gtgctactga tgttagcact
     8521 cagtcaatat cgatatggca aaaggaggaa gtcagaaagg gttgaggccc aagaaccaga
     8581 gttcatttcc gcaatgacac ttaaaaggtt tgatcgaaca gaattagaaa atgctaccgg
     8641 ctccttcagt gagaacaata ttattggtaa cagcagtttg agtactgttt acaagggtcg
     8701 actggaagat ggggagatcg tagcagtgaa aaaactgaat ttccatcaat tctctgcaga
     8761 gtctgacaag agcttctacc gcgaggccaa aaccttaaaa gagctgaagc acaggaatct
     8821 ggttaaggtg ctgggatatg cttgggaaag tgggaaactc aaggcattga ttctggagta
     8881 catggaaaat ggaagcttgg aaagcgttat acatggacct ggtgtggatc actcaaggtg
     8941 gacgctgccc aaaagaatcg atgttcttat ttccgttgcc aatgggttgg attatttgca
     9001 ttcaggttat gacatcccca ttgttcactg tgacatgaag ccttccaata ttctcttaga
     9061 cagagacttt gaggctcatg tgagcgattt tggtacgtct cgaatgttgg atgttcatct
     9121 gcaagacctc tcctcgttat caacagcttt tcaaggcact attggctact tggcaccagg
     9181 taaatctttc gagctcatca gcgtttcctt attacaggtc ttgatttcta tgtaagtctt
     9241 gtacttaatc atatatgtgt tcttttctta tcagaatttg cgtatatgag gacagtcacg
     9301 actaaagtag atgtgtttag ctttggtata gtggtcatgg agttcttgac aaaacggagg
     9361 ccaacaggac tggatgaaga gaatgggttg tcgcctatca gcttgcgtca acttgtggag
     9421 aaaggtttgg caaatggaat caacggggtt cgtcaaatca cggaccctaa actggttttg
     9481 agtaattatg aagagcagga gcagcaccaa gtattggaag agctctttaa actagcctta
     9541 gtttgtactt cctcaaatcc tgaggatcgt cccaatatga atgaggtgct gtctatgctt
     9601 cagaagctaa gagaaacaag atttggaaat cgagatgaac actttccttt cgatgaagga
     9661 gcctcaaaca ctacagcgcc tttaaaagaa cagttctata agggtgggcg cgccgaccca
     9721 gctttcttgt acaaagtggt tgataacagc gggttaatta acatctttta cccatacgat
     9781 gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatc ctatccatat
     9841 gacgttccag attacgctgc tcagtgcagc gcttagagct cgaatttccc cgatcgttca
     9901 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     9961 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
    10021 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
    10081 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
    10141 gatcgggaat tagcttcatc aacgcaagac atgcgcacga ccgtctgaca ggagaggaat
    10201 ttccgacgag cacagaaagg acttgctctt ggacgtaggc ctatttctca ggcacatgta
    10261 tcaagtgttc ggacgtgggt tttcgatggt gtatcagccg ccgccaactg ggagatgagg
    10321 aggctttctt ggggggcagt cagcagttca tttcacaaga cagaggaact tgtaaggaga
    10381 tgcactgatt tatcttggcg caaaccagca ggacgaatta gtgggaatag cccgcgaata
    10441 tctaagttat gcctgtcggc atgagcagaa acttccaatt cgaaacagtt tggagaggtt
    10501 gtttttgggc ataccttttg ttagtcagcc tctcgattgc tcatcgtcat tacacagtac
    10561 cgaagtttga tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
    10621 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
    10681 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
    10741 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
    10801 tttattgcca aatgtttgaa cgatctgctt cgacgcactc cttctttact ccaccatctc
    10861 gtccttattg aaaacgtggg tagcaccaaa acgaatcaag tcgctggaac tgaagttacc
    10921 aatcacgctg gatgatttgc cagttggatt aatcttgcct ttccccgcat gaataatatt
    10981 gatgaatgca tgcgtgaggg gtatttcgat tttggcaata gctgcaattg ccgcgacatc
    11041 ctccaacgag cataattctt cagaaaaata gcgatgttcc atgttgtcag ggcatgcatg
    11101 atgcacgtta tgaggtgacg gtgctaggca gtattccctc aaagtttcat agtcagtatc
    11161 atattcatca ttgcattcct gcaagagaga attgagacgc aatccacacg ctgcggcaac
    11221 cttccggcgt tcgtggtcta tttgctcttg gacgttgcaa acgtaagtgt tggatcccgg
    11281 tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
    11341 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
    11401 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
    11461 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
    11521 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
    11581 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
    11641 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
    11701 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
    11761 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
    11821 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
    11881 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
    11941 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
    12001 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
    12061 gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
    12121 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
    12181 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
    12241 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
    12301 gagttcaggc tttttcatat cggggtcgtc ctctccaaat gaaatgaact tccttatata
    12361 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
    12421 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    12481 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    12541 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    12601 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    12661 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    12721 acgagagtgt cgtgctccac catgttgacg gatctctagg acgcgtcgac gcgtcgacgc
    12781 gtcgacgcgt cgacgcgtcc tagaagctaa ttcactggcc gtcgttttac aacgtcgtga
    12841 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    12901 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    12961 tggcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    13021 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    13081 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    13141 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    13201 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
    13261 cggaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
    13321 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
    13381 aagaaaaacc accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
    13441 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
    13501 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
    13561 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
    13621 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
    13681 acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga gatccgaatt
    13741 atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg
    13801 ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc gctatttctt
    13861 tagaagtgaa cgttgacgat atcaactccc ctatccattg ctcaccgaat ggtacaggtc
    13921 ggggacccga agttccgact gtcggcctga tgcatccccg gctgatcgac cccagatctg
    13981 gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac
    14041 caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg
    14101 gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt
    14161 cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg
    14221 cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg
    14281 acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa
    14341 atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac
    14401 acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag
    14461 accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg
    14521 cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc
    14581 ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa
    14641 acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac
    14701 agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca
    14761 gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga
    14821 gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc
    14881 cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg
    14941 tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg
    15001 cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca
    15061 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
    15121 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
    15181 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
    15241 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
    15301 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
    15361 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
    15421 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
    15481 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
    15541 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
    15601 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
    15661 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
    15721 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
    15781 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
    15841 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
    15901 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
    15961 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
    16021 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
    16081 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
    16141 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
    16201 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
    16261 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
    16321 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
    16381 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
    16441 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
    16501 gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    16561 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    16621 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    16681 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    16741 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    16801 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    16861 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    16921 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    16981 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    17041 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    17101 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    17161 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    17221 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    17281 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
    17341 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
    17401 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    17461 acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg ccaacttact
    17521 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
    17581 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
    17641 gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc
    17701 aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa
    17761 cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc
    17821 gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga
    17881 cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga
    17941 cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg
    18001 gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc
    18061 gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg
    18121 aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct
    18181 gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa
    18241 acactatcaa taagttggag tcattaccca attatgatag aatttacaag ctataaggtt
    18301 attgtcctgg gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga
    18361 tatattgata agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc
    18421 ttctatataa aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct
    18481 cctcatcctc ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta
    18541 attctgtaaa ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa
    18601 atggttcgct gggtttatcg cacccccgaa cacgagcacg gcacccgcga ccactatgcc
    18661 aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg ccccgacggc
    18721 cgaagtgaag ggcaggccgc cacccaggcc gccgccctca ctgcccggca cctggtcgct
    18781 gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat
    18841 cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg ctgccatttt
    18901 tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt
    18961 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
    19021 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
    19081 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
    19141 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
    19201 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
    19261 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
    19321 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
    19381 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
    19441 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc
    19501 tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg gccgccagcc
    19561 cagcggcgag ggcaaccagc ccgg
//
