LOCUS       sequence-399704-       19577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 'Washington navel' Orange FLS2-2 gene under the UBQ10 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 19577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:WNFLS2-2
FEATURES             Location/Qualifiers
     source          1..19577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9709..9733)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9762..9851
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      9885..10137
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(10566..10818)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11286..12311)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(11286..12311)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(12321..12665)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12357..12377)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(12807..12824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12807..12823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12816..12838)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12963..12982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13173..13194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    13441..13465
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(15061..16209)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16508..17302)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17494..18261
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(18954..19577,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatgg agtctcaaac agtcagctta agcttaatcg tagtcatatt
     6061 tttttctctc ttttccactg ttgcattatc acctgcagaa cgaagcttag aagaagtaga
     6121 aactgaagcc ttgaaggcct ttaagaatgg catcaccagc gaccctttag gagcacttgc
     6181 tgattggaat gccacaaatc aaattcatca ctgcaactgg tctggaatca cctgtgacca
     6241 ttcgtcaaac catgtcattg ccattaaact cgtcgacaag cagctccaag gccagatatc
     6301 tccattcctt ggcaaccttt cagccctcca ggttctcgat ttatctttga attcattctc
     6361 tgggagtatt ccggctcagc tggggcaatg ctcccagctt gctgaactaa ctctatacta
     6421 taattctctt tcgggttcca ttccaccaga aataggatcc cttcagaatc tgcaagcaat
     6481 ggacttagga aagaacttct tgaagggaag catacccgaa agcatttgca actgtacttc
     6541 cttgttagcg cttggtctca ttttcaacaa tcttactggt acaatcccaa aagacatcgg
     6601 aaatttgata agccttcaaa tgtttgtagc ataccacaac aggcttgtag gttcaattcc
     6661 agtttccatg ggtagattag aagccttgca agctttagat gtgagtcaga acatgttgtc
     6721 gggaacaata cctctagaaa taggaaacct gtcaaactta gaatatcttc agttatttga
     6781 aaattctatt ggaggaagaa tcccgtccca attaggcaat tttagaaagc ttctcgcgct
     6841 tgaactgtac accaatcagc tcacgggaag cattccctct gagcttggaa atttggctag
     6901 cttacaatca atgcatctac acgaaaatag attgaattcc acaatcccaa tttctttgtt
     6961 ccagctgaaa tctttaactc gtttaggact ctcccagaat gagttgaccg gaacattacc
     7021 ccttgagcta ggatatttaa ggtcattacg agtattgacc ctacattcca ataagttcac
     7081 cggggagatc ccttcttcac tgacaaacct gacaaattta acatatttgt caatgagctt
     7141 caattcctta acagggaagc ttccatcaaa catcgggttg cttcataacc tcgaaaatct
     7201 gaccatgaat aacaatcttc ttaagggaac cattccatct agtatcacca actgtaccca
     7261 tctcgcatcc ataggtttgg cattcaatag gattactggg agaattccca ctggtttagg
     7321 aaagatgcaa aaccttacat tcctatcttt gtcatcaaat ggtatgtccg gagaaatccc
     7381 cgatgatctt ttcaattgct caaatcttga aattctcgat ctgagtgaaa acaatttcag
     7441 tggactgctg aaaccaggaa tcggaaagct taataatctc aagattatga aaatccgaac
     7501 aaattccttt attgggccaa taccaccaga gatcggcaat ctgagccaac tcatgacctt
     7561 gtctcttgct gaaaatagtt tcacaggtcc tattacatca gagttatcca agctttctca
     7621 ccttcaagga ctctctttgc atcacaaccg tctagaaggt gcaatgcctg acagactatt
     7681 tgaactgaag cagctaacct atcttgattt gcagcaaaac aagttgacag gtccaattcc
     7741 aaatgttgtc tcaaatcttg ggtttctttc atacttgaac ctccaaggca acaatcttaa
     7801 cgggtcaata ccaataagca tggagcgtct tcatcgactt ttgaccctgg atctctctca
     7861 taaccattta acaggatccg ctccaggatc tatgattgca ggtatgggaa gtatacaaat
     7921 atatttgaac ttctcataca atttattgga gggaagtatt ccagatgaga ttggtaagct
     7981 ggaaatggca caagccattg acatttcaaa caataacctc tcagggaaaa ttcctaaaat
     8041 gcttcaaggc tgcagaaatc tgttctctct tgatctgtca ggaaataaac tttctggtgc
     8101 aatccctcct gaagtttttg gtcgaatgga tatgcttatg agcttgaacc tttcaaaaaa
     8161 tggtttagac ggtgaaattc ctgaagagtt ggtaaatttg aagcatctta gctcgttgga
     8221 cctttctcac aacaagttga caggcatcat ccctgaaagc ctggccaatc tttcctcctt
     8281 gaaacaactg aacctttctt tcaatcaact tgaaggccgt attcctgaat ccggcatatt
     8341 cagaagcatt aacatgtcta atttggatgg aaacccagct ctgtgcggaa caaaaactct
     8401 cagagcatgc agctcaacga ggaaaaactc ccatcatttg tccaagagga caaagttggt
     8461 acttggctgc tcagttgctg ttgttctgat tctagttctc gtgctactga cgttagcact
     8521 cagtcgatat cgatatggca aaaggaggaa gtcagaaagg gttgaggccc aagaaccaga
     8581 gttcatttcc gcaacgacac ttagaaggtt tgatcgaaca gaattagaaa atgctaccgg
     8641 cttcttcagt gaaaacaaca ttattggtaa cagcagtttg agtactgttt acagaggtcg
     8701 actggaagat gggcagattg tagcagtgaa aaaactgaat ttccatcaat tctctgcaga
     8761 gtctgacaag aacttctacc gcgaggccaa aactttatcg aagctgaagc acaggaatct
     8821 ggttaaggtg ctggggtatg cttgggaaag tgggaaactc aaggcattgg ttcttgaata
     8881 catggaaaat ggaagcttgg aaagcgttat acatggacct ggggtggatc actcaaggtg
     8941 gacgctgccc aaaagaatag atgttcttat ttccgttgcc agtgggttgg attatttgca
     9001 ttctggttat gacttcccca ttgttcactg tgacttgaag ccttccaata ttctcttaga
     9061 cagagacttt gaggctcatg tgagcgattt tggtacgtct cgaatgttgg atgttcatct
     9121 acaagatgtc tcctcattat catcagcttt tcaaggcact attggctact tggcaccagg
     9181 taaatctttc aagctcatca gtgtttcctt attacaggtc atgatttcta tagaagtctt
     9241 gtactaaatc atatatgtgt tctttctcat cagagtttgc gtacatgagg acagtcgcga
     9301 ctaaagtaga tgtgtttagc tttggtatag tggtcatgga gttcttgaca aaacggaggc
     9361 caacaggact ggatgaagag aatgggttgt cgcctatcag cttgcgtcaa cttgtggaga
     9421 aagctgtggc aaatggaatc aacgggattc gtcaaatcac agaccctagg ctggttttga
     9481 gtatttatga agagcaggag cagtaccaag tattggaaga gctctttaaa ctggccttgg
     9541 cttgtacttc ctcaaatcct gaggatcgtc ccaatatgaa tgaggtgctg tctacgcttc
     9601 cgaagctacg agaaacaaga tttggaaatc gtcatgaaca ctttcctttc ggtggagaat
     9661 caaacactac agcgccttta aaaggacagt tcaagggtgg gcgcgccgac ccagctttct
     9721 tgtacaaagt ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg
     9781 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     9841 cagattacgc tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt
     9901 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     9961 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
    10021 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
    10081 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
    10141 aattagcttc atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac
    10201 gagcacagaa aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg
    10261 ttcggacgtg ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt
    10321 cttggggggc agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg
    10381 atttatcttg gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt
    10441 tatgcctgtc ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg
    10501 ggcatacctt ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt
    10561 tgatcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
    10621 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
    10681 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
    10741 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
    10801 ccaaatgttt gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta
    10861 ttgaaaacgt gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg
    10921 ctggatgatt tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat
    10981 gcatgcgtga ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac
    11041 gagcataatt cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg
    11101 ttatgaggtg acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca
    11161 tcattgcatt cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg
    11221 cgttcgtggt ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat
    11281 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
    11341 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
    11401 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
    11461 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
    11521 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
    11581 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
    11641 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
    11701 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
    11761 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
    11821 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
    11881 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
    11941 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
    12001 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
    12061 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
    12121 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
    12181 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
    12241 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
    12301 ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag
    12361 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    12421 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    12481 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    12541 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    12601 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    12661 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    12721 tgtcgtgctc caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg
    12781 cgtcgacgcg tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
    12841 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    12901 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc
    12961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    13021 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    13081 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    13141 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    13201 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggaacc
    13261 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
    13321 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
    13381 accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    13441 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    13501 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    13561 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    13621 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    13681 cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc
    13741 ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat
    13801 aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt
    13861 gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc
    13921 cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga
    13981 gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga
    14041 agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg
    14101 taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg
    14161 ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca
    14221 gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat
    14281 ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc
    14341 ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca
    14401 tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa
    14461 taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc
    14521 accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg
    14581 cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg
    14641 acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa
    14701 tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg
    14761 cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct
    14821 tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa
    14881 tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg
    14941 cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc
    15001 ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
    15061 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
    15121 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
    15181 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
    15241 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
    15301 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
    15361 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
    15421 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
    15481 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
    15541 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
    15601 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
    15661 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
    15721 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
    15781 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
    15841 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
    15901 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
    15961 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
    16021 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
    16081 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
    16141 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
    16201 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
    16261 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
    16321 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
    16381 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
    16441 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
    16501 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
    16561 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
    16621 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
    16681 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
    16741 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
    16801 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
    16861 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
    16921 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
    16981 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
    17041 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
    17101 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    17161 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    17221 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    17281 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    17341 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    17401 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    17461 atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag
    17521 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
    17581 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct
    17641 ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg
    17701 tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg
    17761 cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg
    17821 gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag
    17881 tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc
    17941 cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt
    18001 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
    18061 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
    18121 cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc
    18181 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
    18241 caataagttg gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc
    18301 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
    18361 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
    18421 taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc
    18481 ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt
    18541 aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc
    18601 gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg
    18661 cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg
    18721 aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc
    18781 gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat
    18841 cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg
    18901 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc
    18961 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
    19021 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
    19081 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
    19141 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
    19201 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
    19261 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
    19321 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
    19381 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
    19441 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca
    19501 cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc
    19561 gagggcaacc agcccgg
//
