LOCUS       sequence-399705-       17908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express citrus 'Tango' mandarin LYK5 gene under the UBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Trinh J, Li T, Franco JY, Toruno TY, Stevens DM, Thapa SP, Wong J, 
            Pineda R, Avila de Dios E, Kahn TL, Seymour DK, Ramadugu C, Coaker 
            GL
  TITLE     Variation in microbial feature perception in the Rutaceae family 
            with immune receptor conservation in citrus.
  JOURNAL   Plant Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263.
  PUBMED    37144828
REFERENCE   2  (bases 1 to 17908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 May 5:kiad263. doi: 10.1093/plphys/kiad263."
COMMENT     Sequence Label: pUBQ10:LYK5_TM-3xHA
FEATURES             Location/Qualifiers
     source          1..17908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5972..5996
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8040..8064)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8093..8182
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      8216..8468
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8897..9149)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9617..10642)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9617..10642)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10652..10996)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10688..10708)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(11138..11155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11138..11154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11147..11169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11294..11313)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11504..11525
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11772..11796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13392..14540)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14839..15633)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15825..16592
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(17285..17908,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgatca ggatattctt gtttaagatg
     4981 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     5041 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     5101 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     5161 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     5221 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     5281 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
     5341 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
     5401 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
     5461 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
     5521 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
     5581 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
     5641 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     5701 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     5761 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     5821 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     5881 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     5941 ctctctaccg tgatcaaggc tagagttatc aacaagtttg tacaaaaaag caggctccgc
     6001 ggccgccccc ttcaccatgt ctgctaaatt ctgccggtgc tggttggttc aagttgaagt
     6061 ggtgttggtg ttggtctggt caatacaagg gcaacaaaca tacgtagaca accatcagct
     6121 ggcctgctac gacccgaggt acaacaacat aaccagagga ttcgattgca acggattgta
     6181 tccatcgtgt caagcttacc tgactttccg atcaaatcct tcttacaaca caccagtcac
     6241 catcgactat ctcttcaaga cctcccaccc aaatctcatc gcctccatca acagcataac
     6301 caacgtcact gccaccttac caaccgacac cccggtgctg atccctgtca actgctcctg
     6361 ttccggcggc ggtgacggtg actactacca gttcaacacc acttacacga ttcaaaacca
     6421 cgtggagact tacttaagtg tggctaacaa cacctaccaa ggactaacca cttgccaggc
     6481 catgatgtct cagaacccag tggattctag gaatctcact gtgggtttgg atttgtttgt
     6541 tcccttgagg tgtgcttgtc cttctcgaga ccaggcagct tcaggtttca accatctttt
     6601 aacttacatg gtcacctggg gtgatagcat ctctgcaatt gcacaacttt tcaacgtcga
     6661 cgagcggagc gttcttgacg ctaataagct gtcacaagat gacctcatat ttcccttcac
     6721 tccaattcta gttcctctta aaactgcccc cagcaagatc cagctacctg taccatctcc
     6781 tccaccatct tctccacaca caacgctcac tcctccatct cactcctcca cctcttctaa
     6841 gaaatgggtc ttcataggtg ctgggatcgg agctttcttg cttctgcttg ttgccacctt
     6901 attcgcattt ctattttgcc tctaccgtcg tcgtcgcaac tctaagaaga accccacgcc
     6961 agtgcttact ccaggacgag tcccaccgcc taagactcca ttagattgtg ctgattactc
     7021 gctatttcct caagccagca actccctctc ccacccgcaa gggtttcgaa gtgctgtcga
     7081 gtctttaact ctctacaaat ttcaagattt gaaaattgcc actgggagtt ttagtgaaga
     7141 gaatagaatc cagggctctg tgtaccgggg gtctttcaaa ggtgatgatg ctgccgttaa
     7201 ggtgatgaag ggggatgtct caagcgagat caacattttg aagaagatca atcacagtaa
     7261 cataatacgg ctttctggtt tttgcgttca tgaaggaaac acctaccttg tttatgagtt
     7321 tgcagacaat ggtgctctca gtgactggct ccactctaac aggtaccaga cttctgataa
     7381 tctaacatgg aagcagcgtg ttcagattgc ttacgatgtg gctaatgctc tcaattacct
     7441 ccacaagtac accaatcccc catatgtcca caagaacttg aagaccagta atattctctt
     7501 ggataccaac ttgagagcca agattactaa tttcgggttg gcaagaagtg cagagagcga
     7561 tgagcatgag caggggggtt acggactgca attgacaagg catgtggttg gtacttatgg
     7621 ttacatggcc ccagagtata ttgaaaatgg agtgatcaca cctaaactag atgtttttgc
     7681 cttcggggtt gtggtattgg agctcttatc cggaagggaa gctgtaactg gtgatcagaa
     7741 ttgtggagcg gaattgctat atgcatcaat tagtagggtg cttgaagaaa gcaatgtgag
     7801 agaaaaactc agaggcttca ttgatccttc attgaggaat gaataccccc tagacttggc
     7861 tttctccatg gctcagcttg ctaaaaactg cacagctcat gatctaaatg ctcgcccatc
     7921 catttccgaa gtcttcgtca ctctctctaa gatttggtct tcctcgtcag attgggatcc
     7981 atctgatgag ctaaataact ccaggtcact cagcagaggc agcaagggtg ggcgcgccga
     8041 cccagctttc ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata
     8101 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
     8161 atatgacgtt ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg
     8221 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     8281 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     8341 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     8401 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     8461 actagatcgg gaattagctt catcaacgca agacatgcgc acgaccgtct gacaggagag
     8521 gaatttccga cgagcacaga aaggacttgc tcttggacgt aggcctattt ctcaggcaca
     8581 tgtatcaagt gttcggacgt gggttttcga tggtgtatca gccgccgcca actgggagat
     8641 gaggaggctt tcttgggggg cagtcagcag ttcatttcac aagacagagg aacttgtaag
     8701 gagatgcact gatttatctt ggcgcaaacc agcaggacga attagtggga atagcccgcg
     8761 aatatctaag ttatgcctgt cggcatgagc agaaacttcc aattcgaaac agtttggaga
     8821 ggttgttttt gggcatacct tttgttagtc agcctctcga ttgctcatcg tcattacaca
     8881 gtaccgaagt ttgatcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     8941 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     9001 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     9061 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     9121 aaactttatt gccaaatgtt tgaacgatct gcttcgacgc actccttctt tactccacca
     9181 tctcgtcctt attgaaaacg tgggtagcac caaaacgaat caagtcgctg gaactgaagt
     9241 taccaatcac gctggatgat ttgccagttg gattaatctt gcctttcccc gcatgaataa
     9301 tattgatgaa tgcatgcgtg aggggtattt cgattttggc aatagctgca attgccgcga
     9361 catcctccaa cgagcataat tcttcagaaa aatagcgatg ttccatgttg tcagggcatg
     9421 catgatgcac gttatgaggt gacggtgcta ggcagtattc cctcaaagtt tcatagtcag
     9481 tatcatattc atcattgcat tcctgcaaga gagaattgag acgcaatcca cacgctgcgg
     9541 caaccttccg gcgttcgtgg tctatttgct cttggacgtt gcaaacgtaa gtgttggatc
     9601 ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     9661 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     9721 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     9781 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     9841 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     9901 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     9961 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
    10021 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
    10081 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
    10141 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
    10201 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
    10261 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
    10321 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
    10381 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
    10441 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
    10501 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
    10561 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
    10621 cggtgagttc aggctttttc atatcggggt cgtcctctcc aaatgaaatg aacttcctta
    10681 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    10741 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
    10801 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
    10861 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
    10921 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
    10981 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
    11041 gtagacgaga gtgtcgtgct ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg
    11101 acgcgtcgac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
    11161 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    11221 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    11281 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11341 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11401 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
    11461 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    11521 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
    11581 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
    11641 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
    11701 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
    11761 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
    11821 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
    11881 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
    11941 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
    12001 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
    12061 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
    12121 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
    12181 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
    12241 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
    12301 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
    12361 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    12421 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    12481 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    12541 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    12601 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    12661 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    12721 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    12781 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    12841 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    12901 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    12961 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    13021 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    13081 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    13141 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    13201 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    13261 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    13321 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    13381 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    13441 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    13501 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    13561 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    13621 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    13681 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    13741 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    13801 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    13861 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    13921 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    13981 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    14041 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    14101 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    14161 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    14221 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    14281 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    14341 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    14401 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    14461 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    14521 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    14581 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    14641 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    14701 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    14761 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    14821 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    14881 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    14941 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    15001 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    15061 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    15121 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    15181 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    15241 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    15301 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    15361 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    15421 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    15481 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    15541 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    15601 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    15661 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    15721 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    15781 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    15841 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    15901 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    15961 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    16021 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    16081 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    16141 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    16201 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    16261 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    16321 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    16381 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    16441 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    16501 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    16561 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    16621 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    16681 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    16741 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    16801 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    16861 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    16921 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    16981 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    17041 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    17101 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    17161 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    17221 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    17281 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    17341 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    17401 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    17461 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    17521 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    17581 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    17641 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    17701 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    17761 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    17821 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    17881 agcccagcgg cgagggcaac cagcccgg
//
